Method for detecting platelet-derived growth factor with concentration of 0.5-10[mu]g/mL by using carbon nanotube micro-cantilever biosensor

A platelet-derived, biosensor technology, applied in the field of biomedical engineering, can solve the problems of complex detection methods, difficult to achieve high-sensitivity detection, and inability to meet rapid detection and other problems

Active Publication Date: 2014-10-08
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

Its detection method is complicated to operate and the sensitivity is not high, it is difficult to achieve highly sensitive detection of PDGF in the early stage of tumors, and it cannot meet the needs of rapid detection

Method used

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  • Method for detecting platelet-derived growth factor with concentration of 0.5-10[mu]g/mL by using carbon nanotube micro-cantilever biosensor

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Embodiment 1

[0026] The step that the present invention detects PDGF is as follows:

[0027] (1) Place the carbon nanotube micro-cantilever in a solution containing a nucleic acid aptamer that specifically recognizes PDGF, and modify the nucleic acid aptamer on the carbon nanotubes by π-π superposition by ultrasonic treatment. On the tube, a detection probe comprising a PDGF nucleic acid aptamer is formed;

[0028] (2) When the concentration of the sample to be tested is 0.5-10 μg / mL, drop the sample to be tested onto the carbon nanotube micro-cantilever modified with nucleic acid aptamers, and incubate at room temperature for 15 minutes to make the biosensing interface The nucleic acid aptamer on the aptamer has a specific recognition reaction with the PDGF in the sample to be tested to form a complex;

[0029] (3) The mass size of the formed complex is positively correlated with the concentration of PDGF in the sample to be tested.

[0030] (4) The formed complex produces a mass effect...

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Abstract

A method for detecting a platelet-derived growth factor with the concentration of 0.5-10[mu]g / mL is realized by constructing a carbon nanotube micro-cantilever biosensor. The biosensor comprises a support, a substrate material, a carbon nanotube and a pick-up circuit, and the carbon nanotube is modified with a layer of nucleic acid aptamers through a phi-phi superposing effect. The method comprises the following steps: a detection probe containing a PDGF nucleic acid aptamer is made on a carbon nanotube micro-cantilever, the detection probe is put in a sample to be detected during detection, and PDGF in the sample to be detected and the nucleic acid aptamer on the detection probe form a compound and are attached to the micro-cantilever through a specific reaction; and a micro-cantilever deflection displacement or resonant frequency change caused by the mass change of the compound on the micro-cantilever is positively related to the concentration of PDGF in the sample to be detected in order to realize PDGF detection.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for detecting PDGF with a micro-cantilever beam biosensor. technical background [0002] Platelet-derived growth factor (PDGF), as one of the angiogenesis factors, is closely related to the occurrence and development of tumors, and is considered to be a potential clinically significant tumor marker. At present, there are many methods for detecting PDGF, mainly enzyme-linked immunoassay, immunohistochemistry and so on. Its detection method is complex in operation and low in sensitivity, and it is difficult to achieve highly sensitive detection of PDGF in the early stage of tumors, and it cannot meet the needs of rapid detection. "Nanomechanical microcantilever oprerated in vibratin modes with use of RNA aptamer as a receptor molecules for label-free detection of HCV helicase", Kyo Seon Hwang et al., Biosensors and Bioelectronics, Vol. 23, pp. 459-465, 20070602, publ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/00G01N33/53
Inventor 李桂银周治德梁晋涛黄勇马龙飞管明源黄国银
Owner GUILIN UNIV OF ELECTRONIC TECH
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