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Synergistic effect of ergosterol peroxide and paclitaxel on killing Hela cancer cells and its application

A technology of ergosterol peroxide and cancer cells, which is applied in the fields of natural medicinal chemistry and food chemistry, can solve problems such as infection, fatigue, and neuropathy, and achieve the effects of reducing toxicity, increasing selective inhibition, and reducing costs

Active Publication Date: 2016-06-08
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Paclitaxel-bevacizumab combination can significantly prolong the progression-free survival of metastatic breast cancer compared with paclitaxel alone, but these combined anticancer regimens often have some side effects, such as: neuropathy, infection and fatigue ( MillerK., WangM.M., GralowJ., DicklerM., CobleighM., PerezE.A., ShenkierT., CellaD., DavidsonN.E. (2007).

Method used

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  • Synergistic effect of ergosterol peroxide and paclitaxel on killing Hela cancer cells and its application
  • Synergistic effect of ergosterol peroxide and paclitaxel on killing Hela cancer cells and its application
  • Synergistic effect of ergosterol peroxide and paclitaxel on killing Hela cancer cells and its application

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Embodiment Construction

[0017] cell culture

[0018] Hela (human cervical cancer cells), A431 (human skin cancer cells), GES-1 (human MG53 gastric mucosal epithelial cells), HBE (human bronchial epithelial cells) were cultured in DMEM medium; MGC-803 (human gastric cancer cells) , HUEVC (human umbilical MG53 vein epithelial cells) were cultured in PRMI1640 medium. All media contain 10% calf serum and 100 U / ml penicillin and streptomycin. Cells at 37 °C 5% CO 2 cultured in an incubator.

[0019] cytotoxic activity

[0020] Cells were seeded on a 96-well plate with 5000 cells per well. After the cells adhered to the wall, compounds were added to continue culturing for 72 hours. 20 μl MTT (5 mg / ml) was added to each well and cultured for 4 hours. After the culture medium was aspirated, 150 μl DMSO was added and the absorbance was measured at 570 nm. .

[0021] figure 2 Shown are the inhibitory rates of paclitaxel and ergosterol peroxide on the proliferation of Hela cells when paclitaxel and ergos...

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Abstract

The invention relates to a composition for killing Hela cancer cells. The composition is composed of ergosterol peroxide and paclitaxel, and has the effect of synergistically inhibiting the proliferation of Hela cancer cells, especially in the concentration range of 6.25-12.5 μg / ml ergosterol peroxide: 0.025-0.1 μg / ml paclitaxel. significantly. The composition can greatly reduce the dosage of paclitaxel, reduce cost and side effects, and may be effective for cancer cell lines resistant to paclitaxel.

Description

technical field [0001] The invention belongs to the technical fields of natural medicinal chemistry and food chemistry, and relates to two natural products: ergosterol peroxide and paclitaxel. Ergosterol peroxide can enhance the inhibitory effect of paclitaxel on cancer cell proliferation. Background technique [0002] Ergosterol peroxide (EP) ( figure 1 )是药用真菌及食用蘑菇中广泛存在的天然成分(ChenY.K.,KuoY.H.,ChiangB.H.,LoJ.M.,SheenL.Y.(2009).Cytotoxicactivitiesof9,11-dehydroergosterolperoxideandergosterolperoxidefromthefermentationmyceliaofganodermalucidumcultivatedinthemediumcontainingleguminousplantsonHep3Bcells.Journalofagricultural&foodchemistry ,57(13),5713-5719.;KoboriM.,YoshidaM.,Kameyama,M.O.,Shinmoto.H.(2007).ErgosterolperoxidefromanediblemushroomsuppressesinflammatoryresponsesinRAW264.7macrophagesandgrowthofHT29colonadenocarcinomacells.BritishJournalofPharmacology,150(2),209-219)。 Ganoderma lucidum and other medicinal fungi have been used as food supplements for cancer patients ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/58A61P35/00A61K31/337
Inventor 马超美高杰郑华川阿拉坦高勒
Owner INNER MONGOLIA UNIVERSITY
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