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Hericium erinaceus glycoprotein with anti-tumor and agglutination activity and preparation method thereof

A technology of glycoprotein and Hericium erinaceus, which is applied in the field of Hericium erinaceus glycoprotein and its preparation, achieves the effects of high extraction rate, broad application prospects, and simple preparation process

Inactive Publication Date: 2014-10-01
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the glycoprotein components of Hericium erinaceus, and only the patent (CN 1772287) discloses the application of glycoproteins from Hericium erinaceus fermentation extract in pharmaceuticals

Method used

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  • Hericium erinaceus glycoprotein with anti-tumor and agglutination activity and preparation method thereof
  • Hericium erinaceus glycoprotein with anti-tumor and agglutination activity and preparation method thereof
  • Hericium erinaceus glycoprotein with anti-tumor and agglutination activity and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0036] Cultivate the strain Hericium erinaceus ACCC 50011 preserved in the China Agricultural Microorganism Culture Collection and Management Center on the substrates such as soybean straw powder, corn stalk powder, wheat bran, broad-leaved tree bran, sugar beet bagasse, sugarcane bagasse or cottonseed hulls, etc., for 20-30 days. oC After culturing for 15-30 days, the fruiting bodies of Hericium erinaceus were collected.

Embodiment 2

[0038] Activate the strain Hericium erinaceus ACCC 50011 preserved in the China Agricultural Microbiological Culture Collection Management Center, boil it in PDA slant medium (200 g potato in boiling water for 20 min, filter the filtrate, add 20 g glucose and 20 g agar, and add water to 1 L, pH natural). The test tubes and tampons used for culture need to be kept at 121 oC Sterilize for 30 min, then fill with medium 121 oC Sterilize for 30 minutes, and cool down on an inclined plane to make it solidify into an inclined plane. Then the ultra-clean bench was inoculated. The slant was cultured at a constant temperature of 25 oC Left and right, for a period of 7 days. Cut the slant bacteria into small pieces and inoculate them into the seed culture medium. After the medium is divided, put a cotton plug on the Erlenmeyer flask to prevent external microorganisms from entering the medium and cause pollution, and ensure good ventilation performance. . The composition of the s...

Embodiment 3

[0040] After 500 g Hericium erinaceus fruiting bodies were added to 0.8 times the volume of water, the homogenate was broken, 4 o C leaching, centrifugation, discarding the insoluble matter, collecting the supernatant, adding ammonium sulfate to 80% saturation after the supernatant was concentrated, standing for 24 hours, high-speed centrifugation to take the precipitate, adding a small amount of water to redissolve the above precipitate, and reconstituted The solution was placed in a dialysis bag of 8000-14000 Da, dialyzed with deionized water, the dialysate was collected, concentrated, subjected to DEAE-Sepharose Fast Flow column chromatography, unbound components were eluted with distilled water, and 0.1 mol / L, 0.3 mol / L and 0.5 mol / L NaCl were used as the eluent for gradient elution, and the glycoprotein fractions eluted at each stage of the NaCl solution were collected, and each collected solution was freeze-dried; among them, the HE-1 fraction was subjected to Superdex T...

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Abstract

The invention discloses hericium erinaceus glycoprotein with anti-tumor and agglutination activity and a preparation method thereof, and belongs to the technical field of active protein preparation. The preparation method comprises the following steps: with mycelium prepared by submerged fermentation of hericium erinaceus or sporocarp obtained by a solid cultivation technology as the raw material, separating and purifying to obtain the hericium erinaceus glycoprotein by virtue of steps such as homogenizing and crushing, leaching in cold water, centrifuging, taking liquid supernatant, precipitating ammonium sulfate, carrying out dialysis, and carrying out DEAE (diethyl-aminoethanol)-SepharoseFastFlow column chromatography and the like. Content of polysaccharides in the glycoprotein is 2-20%, and content of protein with a molecular weight range of 10-80KDa is 80-90%. The glycoprotein disclosed by the invention has agglutinating activity on red blood cells, and has a remarkable inhibiting effect on growth of tumor cells such as liver cancer cells, stomach cancer cells and breast cancer cells, and can be used for preparing a possible anti-cancer drug or can be developed into a health product.

Description

[0001] Technical field The present invention belongs to the technical field of active protein preparation, and relates to a Hericium erinaceus glycoprotein with anti-tumor activity and agglutination activity and a preparation method thereof. Background technique [0002] Edible and medicinal fungi have various medicinal values ​​such as anti-tumor, lowering blood fat, regulating body immunity, and protecting cardiovascular and cerebrovascular. The separation, purification, and structure determination of their active components are one of the current research hotspots. Active ingredients that have been isolated from it include: polysaccharides, glycoproteins, terpenoids and phenolic compounds, etc. In recent years, with the in-depth study on the isolation of polysaccharide complexes of edible fungi, it has been found that they have significant anti-tumor effects. For example: PCP-3A, a glycoprotein isolated from the dry fruiting body of Pleurotus aureus, at a concentration of 5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/375C07K1/36C07K1/30C07K1/16A61K38/16A61P35/00A61P7/00
CPCC07K14/375
Inventor 崔凤杰李云虹昝新艺孙文敬钱静亚张志才许宁
Owner JIANGSU UNIV
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