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Preparation method and usage of Asiatic toddalia root total alkaloid effective part extract

A technology of total alkaloids and effective parts is applied in the preparation of extracts from effective parts of total alkaloids in the blood of Fenugreek, and in the field of preparing anti-tumor drugs, which can solve the problems of cytotoxicity and tumor cell apoptosis in Fenugreek blood, and achieve inhibition of The economic effect of tumor cell proliferation and purification process

Inactive Publication Date: 2014-10-01
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Existing documents 1 and 2 involve the in vitro anti-tumor experiments on dihydrophotoxanthin and its derivatives extracted from the blood of Pythias chinensis produced in Okinawa, Japan, showing that the above components can induce apoptosis of lung cancer (A549), and confirmed that The blood of Feilongzhang in this region is cytotoxic and can induce apoptosis of tumor cells

Method used

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  • Preparation method and usage of Asiatic toddalia root total alkaloid effective part extract
  • Preparation method and usage of Asiatic toddalia root total alkaloid effective part extract

Examples

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Effect test

Embodiment 1

[0038] Weigh 50g of the pulverized Pterodactylus japonicus root, immerse in 70% ethanol for 12h, and then heat extract twice, each time the amount of solvent is 10 times, each extraction time is 2 hours, combine the two extracts, concentrate to a thick extract, Vacuum-dried to obtain the effective part extract of Feilongzhang Blood Alcohol Extraction of the present invention, weighing 5.091g, and the content of limpetine chloride measured by high performance liquid phase is 0.183mg.

Embodiment 2

[0040] Weigh 5 g of the pulverized blood root of Flying Dragon palm, impregnate it with 70% ethanol for 12 h, then heat extract 3 times, the amount of solvent is 10 times each time, and the extraction time is 60 min each time, combine the extracts for 3 times, concentrate until thick paste, use 1 mol / L hydrochloric acid to adjust the pH of the sample solution to 2, the concentration of the sample solution is 0.1 mg crude drug / ml, pass through 30 ml D001 type cation-exchange macroporous resin at a flow rate of 1.5 BV / h, and successively wash with 500 ml water Impurities were removed by elution, and then eluted with about 300 ml of 95% ethanol-ammonia water (9:1), the eluate was collected, concentrated to a thick extract, and dried in vacuum to obtain the effective position extract, the content of total alkaloids in the extract from the three batches of samples is shown in Table 1,

[0041] Table 1 Validation of extraction and purification process

[0042] serial numb...

Embodiment 3

[0044] In vitro inhibition of the proliferation of human tumor cells A549, AGS, SGC-7901, SMMC-7721, the tumor cells fused into a monolayer were digested with 0.25% trypsin, counted and adjusted to a cell concentration of 5×10 4 cells / mL, inoculated in 96-well plate, 90 μL / well, set negative control group, positive control group and blank control group (only culture medium, no cells and drugs) at the same time, placed in 37 ℃, 5% CO 2 After culturing in the incubator for 24 hours, 10 μL of test sample solution of different concentrations were added to the experimental group, 10 μL of 5-fluorouracil (5-Fu) solution of different concentrations were added to the positive control group, and 10 μL of 5-fluorouracil (5-Fu) solution of different concentrations were added to the experimental group. Add 10 μL of PBS and continue the culture; after 48 h, add 10 μL of MTT to each well, continue to culture in the incubator, stop the culture after 4 h, suck off the culture supernatant in t...

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Abstract

The invention belongs to the field of Chinese pharmaceutical manufacturing, and relates to a preparation method and usage of an Asiatic toddalia root total alkaloid effective part extract. The Asiatic toddalia root total alkaloid extract with high total alkaloid content, controllable and stable quality and remarkable pharmacological function is prepared by taking the content of the Asiatic toddalia root total alkaloids as the index. Among the Asiatic toddalia root total alkaloids effective part extract, the content of the total alkaloids is above 50%. The preparation method is economical, simple and convenient in extraction process and suitable for industrial production. The prepared extract has a remarkable effect for inhibiting tumor cell growth, can induce tumor cell apoptosis and can be used for preparing anti-tumor medicines.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a preparation method and medicinal use of an extract of the effective part of total alkaloids in the blood of Feilongzhang, and in particular to a method for preparing the extract of effective parts of the total alkaloids in Feilongzhang blood and its use in the preparation of antitumor drugs . Background technique [0002] It is disclosed in the prior art that Feilongzhangxue is a commonly used ethnic medicine blindly recorded in the annals of medicines of ethnic minorities such as Miao and Tujia, and is a Rutaceae plant Feilongzhangxue. Toddalia asiatica Dried root of Lam.; its taste is pungent, slightly bitter, warm in nature, slightly poisonous, and has the functions of dispelling wind and relieving pain, dispelling blood stasis to stop bleeding, detoxifying and reducing swelling. Miao medicine often uses the blood of Feilongzhang together with Imperata Rh...

Claims

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Application Information

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IPC IPC(8): A61K36/75A61P35/00
Inventor 朱国福范圣洁高玉琼
Owner SHANGHAI UNIV OF T C M
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