Skin-protecting composition and cosmetics with oil-controlling and pore-shrinking effects
A skin care composition and pore shrinking technology, applied in cosmetics, cosmetic preparations, hair care, etc., can solve the problems of less obvious pores, difficulty in having efficacy, shrinking pores, etc., achieve long-term stability of internal quality, improve appearance, inhibit The effect of oil secretion
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Embodiment 1
[0028] Example 1, preparation of Viola didin extract, Anemarrhena anemarrhena extract, rhubarb extract and composition
[0029] In the present example, Viola chinensis, Anemarrhena anemarrhena, and rhubarb were purchased from pharmacies. According to the following methods, respectively prepare the extracts of viola radix, anemarrhena anemarrhena, and rhubarb extracts:
[0030] The extract of Viola radix is extracted according to the following method: crush the dried Chinese medicinal material of Viola radix through an 80-mesh sieve, weigh 100 g of Viola radix powder, and use the ratio of 20 mL of extraction solvent per gram of Viola radix to 60% concentration of ethanol as the extraction solvent, reflux at 80 ℃ for three times, each time for 90min, filter the filtrate after each extraction, combine the three filtrates, concentrate to remove ethanol, and then adjust the pH value to 3, add 0.5 per 50ml of the extract g adsorbent, stirred and adsorbed to separate chlorophyll, ...
Embodiment 2
[0034] Embodiment 2, experiment of inhibiting 5α reductase
[0035] 5α-reductase catalyzes the conversion of testosterone into dihydrotestosterone with the participation of reduced coenzyme II. If there is a 5α-reductase inhibitor, this reaction process can be inhibited to a certain extent. Since reduced coenzyme II has a characteristic absorption at a wavelength of 340nm, the characteristic absorption of oxidized coenzyme II produced after participating in the reaction disappears at a wavelength of 340nm. Determination with an ultraviolet spectrophotometer can reflect whether a certain substance has an inhibitory effect on 5α reductase and the size of the inhibitory effect. After repeated trials, a 5α-reductase-catalyzed reaction system with a total volume of 100 μL was established. The reaction volume of each component is shown in Table 1.
[0036] Table 1 The amount of each reagent in the reaction system (μL)
[0037]
[0038] Determination of the blank control: add t...
Embodiment 3
[0044] Embodiment 3, anti-inflammatory experiment
[0045] Get 50 mice, half male and half male, randomly divided into 5 groups, 10 in every group, i.e. blank control (distilled water) group. Ibuprofen control group (concentration of ibuprofen is 0.1mg / g), get a certain amount of implementation The viola extract, anemarrhena extract, rhubarb extract, and skin care composition prepared in Example 1 were diluted to 10 mg / g, and the test group was administered by intragastric administration for 7 consecutive days. 30 minutes after the last administration, 50 μL of xylene was evenly applied to the right ear of the mouse, and the left ear was not applied as a control. Ear pieces were laid at the same position on both ears, and their quality was weighed. Calculate swelling degree and swelling inhibition rate.
[0046] Swelling degree=weight of right ear piece−mass of left ear piece; swelling inhibition rate=(average swelling degree of blank control group−average swelling degree of...
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