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Streptococcus zooepidemicus and production process for preparing hyaluronic acid by using same

A technology of Streptococcus zooepidemicus and hyaluronic acid, which is applied to bacteria, microorganisms, microorganism-based methods, etc., can solve problems such as insoluble insoluble, and achieve the effects of reducing production costs, easy production control, and low impurity content.

Active Publication Date: 2014-09-24
SHANDONG AWA BIOPHARM
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  • Description
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AI Technical Summary

Problems solved by technology

Some hyaluronic acid is fibrous in form and difficult to dissolve

Method used

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Embodiment １

[0037] A kind of Streptococcus zooepidemicus of the present invention and the technical scheme of the production technology of using it to prepare hyaluronic acid are, a kind of Streptococcus zooepidemicus ( Streptococcus zooepidemicus ) AWA008, which was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, its preservation number is: CGMCCNo.9111, and the preservation date is April 29, 2014.

[0038] At present, the Streptococcus zooepidemicus is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, postcode: 100101, and its preservation number is: CGMCCNo.9111, the Latin name of the strain text name is Streptococcus zooepidemicus , The reference microorganism (strain): AWA008, the date of deposit is April 29, 2014.

[0039] The characteristics of the Streptococcus zooepidemicus strain of...

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Abstract

The invention discloses streptococcus zooepidemicus and a production process for preparing hyaluronic acid by using the same. The compound mutation technology is adopted to screen out strains with high hyaluronic acid yields and is combined with the fermentation technology. The yield of the hyaluronic acid product is as high as 7-9g / L. The hyaluronic acid obtained through fermentation is easy to separate and purify and is short in production period. Production is easy to control. The production process is suitable for large-scale industrial production, is high in yield and has the effect of greatly reducing the production cost.

Description

technical field [0001] The invention relates to the technical field of biological polysaccharide extraction, in particular to a Streptococcus zooepidemicus and a production process for preparing hyaluronic acid using it. Background technique [0002] There are currently more than a dozen manufacturers of hyaluronic acid in China, and most of these companies use animal tissue extraction. The commonly used raw materials for extracting HA from animal tissues are cockscomb, umbilical cord and pigskin, etc. The main process includes dehydration, grinding, soaking, extraction, impurity removal, precipitation and separation. This method is characterized by a simple process flow and is suitable for small-scale production where raw material sources are dispersed. However, due to the limited raw materials and the low content of HA in the raw materials, HA also coexists with mucopolysaccharides such as chondroitin sulfate in biological tissues, resulting in low yield, difficult purifi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/26C12R1/46
Inventor 韩秀云李松田
Owner SHANDONG AWA BIOPHARM
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