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Novel method for preparing Qinghai Qaidam lycium barbarum polysaccharide in extraction and separation manner

A technology of Qaidam wolfberry and Lycium barbarum polysaccharide, which is applied in the direction of anti-toxic agent and drug combination, can solve the problems of deep color, low yield and low purity of the product, and achieve the effect of low cost, high extraction efficiency and high purity

Active Publication Date: 2014-09-24
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional extraction and separation of Lycium barbarum polysaccharides mainly adopts the method of water extraction and alcohol precipitation, which usually has a long-term high-heat extraction process, which has disadvantages such as time-consuming, low yield, low purity, and dark product color.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 A new method for extracting, separating and preparing Qinghai Qaidam Lycium barbarum polysaccharides, comprising the following steps:

[0023] ⑴Using 10 Kg of Qinghai Qaidam Lycium barbarum fresh fruit as raw material, washed several times with pure water to remove impurities, and obtained processed Lycium barbarum.

[0024] (2) Soak the treated wolfberry in pure water at room temperature at a ratio of 1g: 3mL, inject the soaked wolfberry and the soaking liquid into a crusher for crushing after 8 hours, and obtain crushed wolfberry.

[0025] (3) Add pure water to the crushed wolfberry at a ratio of 1g:10mL, and carry out circular ultrasonic extraction at a temperature of 20°C, an ultrasonic power of 600 W, and a stirring speed of 600 rpm. The number of extractions is 2 times, and each extraction The time is 40 min, combined to obtain the extract.

[0026] (4) The extract is filtered with a 200-mesh filter cloth to obtain the filtrate and filter residue resp...

Embodiment 2

[0032] Example 2 A new method for extracting, separating and preparing Qinghai Qaidam Lycium barbarum polysaccharides, comprising the following steps:

[0033] ⑴Using 10 Kg of Qinghai Qaidam Lycium barbarum fresh fruit as raw material, washed several times with pure water to remove impurities, and obtained processed Lycium barbarum.

[0034] (2) Soak the treated wolfberry in pure water at room temperature at a ratio of 1g: 4mL, inject the soaked wolfberry and the soaking solution into a crusher for crushing after 10 hours, and obtain crushed wolfberry.

[0035] (3) Add pure water to the crushed wolfberry at a ratio of 1g:10mL, and carry out circular ultrasonic extraction at a temperature of 30°C, an ultrasonic power of 800 W, and a stirring speed of 700 rpm. The number of extractions is 2 times, and each extraction The time is 50 min, combined to obtain the extract.

[0036] (4) The extract is filtered with a 200-mesh filter cloth to obtain the filtrate and filter residue ...

Embodiment 3

[0042] Example 3 A new method for extracting, separating and preparing Qinghai Qaidam Lycium barbarum polysaccharides, comprising the following steps:

[0043] ⑴Using 10 Kg of Qinghai Qaidam Lycium barbarum fresh fruit as raw material, washed several times with pure water to remove impurities, and obtained processed Lycium barbarum.

[0044] (2) Soak the treated wolfberry in pure water at room temperature at a ratio of 1g: 4mL, inject the soaked wolfberry and the soaking solution into a crusher for crushing after 10 hours, and obtain crushed wolfberry.

[0045](3) Add pure water to the crushed wolfberry at a ratio of 1g:10mL, and carry out circular ultrasonic extraction at a temperature of 30°C, an ultrasonic power of 800 W, and a stirring speed of 800 rpm. The number of extractions is 2 times, and each extraction The time is 50 min, combined to obtain the extract.

[0046] (4) The extract is filtered with a 200-mesh filter cloth to obtain the filtrate and filter residue r...

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PUM

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Abstract

The invention relates to a novel method for preparing a Qinghai Qaidam lycium barbarum polysaccharide in an extraction and separation manner. The method comprises the following steps: (1) washing fresh Qinghai Qaidam lycium barbarum fruits taken as raw materials with pure water for multiple times and then removing impurities so as to obtain treated lycium barbarum; (2) soaking the treated lycium barbarum in the pure water at a normal temperature and carrying out smashing so as to obtain the smashed lycium barbarum; (3) carrying out ultrasonic extraction by adding the pure water into the smashed lycium barbarum so as to obtain an extraction liquid; (4) filtering the extraction liquid with a filter cloth so as to respectively obtain a filtrate and filter residues; (5) centrifuging the filtrate so as to respectively obtain a supernate and residues; (6) feeding the supernate to pass through a nonpolar macroporous resin and then cleaning a resin column with the pure water so as to obtain a polysaccharide eluent; (7) removing granular impurities from the polysaccharide eluent via a ceramic membrane so as to obtain a transparent liquid; (8) intercepting the transparent liquid with an organic ultrafiltration membrane so as to obtain an intercepting liquid, carrying out freeze drying on the intercepting liquid to reach a constant weight so as to obtain a brown yellow Qaidam lycium barbarum polysaccharide powder product. The method is simple, convenient, rapid and low in cost.

Description

technical field [0001] The invention relates to a preparation method of Lycium barbarum polysaccharide, in particular to a new method for extraction, separation and preparation of Qinghai Qaidam Lycium barbarum polysaccharide. Background technique [0002] Qaidam wolfberry is mainly produced in the Qaidam Basin on the Qinghai-Tibet Plateau. The unique geographical climate of the Qinghai-Tibet Plateau has created the excellent quality of Qaidam wolfberry, which has the characteristics of large granules, red color, thick flesh, high sugar content, and sweet taste. It is a pure natural and pollution-free high-grade tonic. Modern scientific research proves that wolfberry has the effects of regulating immune function, lowering blood sugar, lowering blood fat, anti-aging, anti-tumor, etc., and its pharmacological effects are closely related to its biologically active component Lycium barbarum polysaccharide LBP. Therefore, using it as a functional factor to develop medical and he...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
Inventor 陈涛李玉林陈晨
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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