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Cultivation method of bee pupa cordyceps militaris

A technology of Cordyceps militaris and cultivation methods, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve environmental problems such as pollution, waste of resources, etc., and achieve the effect of increasing economic benefits, high quality and high content

Active Publication Date: 2014-09-24
陆九妹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a big beekeeping country with abundant resources of bee products. The honey, powder, paste, glue, wax and pupae of most bees have been well developed and utilized. Only the honeycomb is mainly used to extract 25% of it. % beeswax, while other substances containing a large amount of nutrition and medicinal health care are not well developed and utilized, resulting in waste of resources and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Cultivation method of Cordyceps militaris:

[0030] (1) Screening of the Cordyceps militaris bacterial strain, the spores of the Cordyceps sinensis strain are obtained by the method of tissue separation to obtain the pure bacterial strain of Cordyceps sinensis;

[0031] (2) Preparation of honeybee pupae organic medium: use 100g of live protein of live honeybee pupae or peeled honeybee pupae, 200g of rice soup, 10mg of camellia powder, 2g of potassium dihydrogen phosphate, put them into a 1000mL beaker, add 25 g of water and heat Stir for miscibility, stop heating after the completion of miscibility, pack into test tubes, stopper and cool, and then make semi-liquid organic culture medium for bee pupae;

[0032] (3) Production of spore suspension: inoculate the Cordyceps militaris strain cultivated in step (1) on the organic medium of honeybee pupa produced in step (2), and cultivate at 20°C until conidia appear The picked part is placed in sterile water to make a spore ...

Embodiment 2

[0039] Cultivation method of Cordyceps militaris:

[0040] (1) Screening of the Cordyceps militaris bacterial strain, the spores of the Cordyceps sinensis strain are obtained by the method of tissue separation to obtain the pure bacterial strain of Cordyceps sinensis;

[0041] (2) Production of honeybee pupae organic medium: use 100g of live protein of live honeybee pupae or peeled honeybee pupae, 200g of rice soup, 10mg of camellia pollen, 2g of potassium dihydrogen phosphate, put them into a 1000mL beaker, add 35mL of water and heat Stir for miscibility, stop heating after the completion of miscibility, pack into test tubes, stopper and cool, and then make semi-liquid organic culture medium for bee pupae;

[0042](3) Production of spore suspension: inoculate the Cordyceps militaris strain cultivated in step (1) on the organic medium of honeybee pupa produced in step (2), and cultivate at 20°C until conidia appear The picked part is placed in sterile water to make a spore su...

Embodiment 3

[0049] Cultivation method of Cordyceps militaris:

[0050] (1) Screening of the Cordyceps militaris bacterial strain, the spores of the Cordyceps sinensis strain are obtained by the method of tissue separation to obtain the pure bacterial strain of Cordyceps sinensis;

[0051] (2) Preparation of honeybee pupae organic medium: use 100g of live protein of live honeybee pupae or peeled honeybee pupae, 200g of rice soup, 10mg of camellia powder, 2g of potassium dihydrogen phosphate, put them into a 1000mL beaker, add 45mL of water and heat Stir for miscibility, stop heating after the completion of miscibility, pack into test tubes, stopper and cool, and then make semi-liquid organic culture medium for bee pupae;

[0052] (3) Production of spore suspension: inoculate the Cordyceps militaris strain cultivated in step (1) on the organic medium of honeybee pupa produced in step (2), and cultivate at 20°C until conidia appear The picked part is placed in sterile water to make a spore ...

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PUM

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Abstract

The invention discloses a cultivation method of bee pupa cordyceps militaris. According to the method, bee worms are taken as a material, a honeycomb spleen material is taken as a culture medium to ensure high quality and high content of functional components in a bee pupa cordyceps militaris product, a sterilized honeycomb is used to simulate a natural ecological environment of the bee pupa cordyceps militaris, and the bee pupa cordyceps militaris is artificially cultivated. The inoculation success rate and the muscardine rate after inoculation are high, and the bee pupa cordyceps militaris contains a large quantity of active ingredients of cordycepin, cordycepic acids, adenosine, polysaccharides, protein, SOD (superoxide dismutase) and the like, can boost immunity, prevent cancer and tumor, prevent aging, tonify the lung and the kidney and the like, and is a valuable and rare good product for dietary therapy and medicated food for supplementing nutrition and prolonging life.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris. Background technique [0002] Natural Cordyceps is scarce and expensive. Using the method of artificial cultivation, the Cordyceps militaris cultivated by submerged fermentation using mycelium has basically the same medicinal effect as Cordyceps sinensis, so it is beneficial to the cultivation of silkworm chrysalis. Flocks of households flock to cultivate Cordyceps militaris, and the cultivation technology conditions are uneven. , the phenomenon of focusing on high yield and ignoring the efficacy and health care functions appeared in the production, and the price competition in the industry was fierce. [0003] In order to break through the bottleneck that inhibits the development of the pupae industry, we use honeybee pupae with high-quality functional components as materials to lay the foundation for improving the functional components of Cordyceps militaris; High quality and hig...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 陆九妹冷云谷冷雁
Owner 陆九妹
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