Method for preparing Se-enriched rice protein by tissue culture method
A technology of tissue culture and selenium-enriched rice, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low enrichment concentration, low nutritional value, limited enrichment concentration, etc., and achieve high enrichment concentration , high enrichment efficiency and high efficiency
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Embodiment 1
[0029] This embodiment includes the following steps:
[0030] (1) Cleaning: Harvest rice in the same year, after shelling, collect fresh brown rice and wash it with tap water;
[0031] (2) Disinfection: Soak the brown rice cleaned in step (1) with ozone solution (concentration: 2mg / L) for 30 minutes, then wash with sterile water for 3 times, and cycle 3 times for soaking in ozone solution and cleaning with sterile water ;
[0032] (3) Embryo tissue culture: put the sterilized brown rice in step (2) into a sterilized fermentation container, add sterile soaking solution equivalent to 3 times the weight of sterilized brown rice and soak (25°C) for 8 hours, and the aseptic The soaking solution contains plant growth regulators (2,4-D 4.0mg / L, KT1.0 mg / L, 6-BA2.0 mg / L), sodium selenite (the concentration of selenium in the soaking solution is 30mg / L L); then cultured in a shallow fermentation plate at a temperature of 25°C for 24 hours;
[0033] (4) Collect the culture: centrifug...
Embodiment 2
[0038] This embodiment includes the following steps:
[0039] (1) Cleaning: Collect the by-products of the rice germ when the rice is harvested and milled in the same year, and rinse with tap water;
[0040] (2) Disinfection: soak and disinfect the rice germ cleaned in step (1) with chlorine dioxide solution (500mg / L concentration) for 30 minutes, and then wash it with sterile water for 3 times. L) Soaking and washing with sterile water cycle twice;
[0041] (3) Embryo tissue culture: put the sterilized rice germ in step (2) into a sterilized fermentation container, add a sterile soaking solution equivalent to 3 times the weight of the sterilized rice germ, and the sterile soaking solution contains plant growth regulators agent (2,4-D 3.0mg / L, kinetin KT1.0 mg / L, NAA0.2 mg / L, sodium selenite (concentration of selenium in soaking solution is 20mg / L); temperature 20℃, Ferment for 72 hours, 500mL Erlenmeyer bottle liquid 150mL, shaker speed 90r / min;
[0042] (4) Collect the cu...
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Abstract
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