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Method for extracting peony polysaccharide from peony seed meals

A technology of peony seed meal and polysaccharide, applied in the field of plant extraction, to achieve the effect of easy industrial production and high purity

Active Publication Date: 2014-09-10
HEZE YAO & SHUN PEONY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, reports on polysaccharides from peony are rare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting peony polysaccharides from peony seed dregs, the method comprising the steps of:

[0032] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0033] (2) Enzymatic hydrolysis: Add the peony seed meal powder in step (1) to water at a ratio of 1:15 to the solid-liquid ratio, adjust the pH to 5 at 45°C, and add cellulase and pectinase:

[0034] The added cellulase accounts for 0.15% of the weight of the material, and its enzyme activity is ≥80,000 U / g;

[0035] Pectinase 0.15%, its enzyme activity ≥ 100,000 U / g, hydrolyze for 2 hours, inactivate the enzyme at 95°C for 8 minutes to obtain the hydrolyzate;

[0036] (3) Ethanol extraction: Take the enzymolysis slurry in step (2), add 95% ethanol solution, make the ethanol volume fraction in the mixed solution of enzymolysis solution and ethanol solution...

Embodiment 2

[0042] A method for extracting polysaccharides from peony seed meal, the method comprises the following steps:

[0043] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0044] (2) Enzymatic hydrolysis: add peony seed meal powder in step (1) to water at a ratio of 1:10 to liquid, adjust the pH to 4 at 35°C, add cellulase and pectinase, the added The cellulase accounted for 0.1% of the weight of the material, its enzyme activity ≥ 80,000 U / g, the pectinase 0.1%, its enzyme activity ≥ 100,000 U / g, enzymatic hydrolysis for 1 h, inactivated at 95 ° C to obtain enzymatic solution;

[0045] (3) Ethanol extraction: take the enzymatic hydrolysis slurry in step (2), add 95% ethanol solution, make the ethanol volume fraction in the mixed solution of enzymolysis solution and ethanol solution be 45%, and perform ultrasonic extraction, the...

Embodiment 3

[0051] A method for extracting peony polysaccharides from peony seed dregs, the method comprising the steps of:

[0052] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0053] (2) Enzymolysis: Add the peony seed meal powder in step (1) to water at a ratio of solid to liquid 1:20, adjust the pH to 6 at 45°C, add cellulase and pectinase, the added The cellulase accounted for 0.2% of the weight of the material, its enzyme activity ≥ 80,000 U / g, the pectinase 0.2%, its enzyme activity ≥ 100,000 U / g, enzymatic hydrolysis for 3 hours, and inactivated at 95°C to obtain enzymatic solution;

[0054] (3) Ethanol extraction: Take the enzymolysis slurry in step (2), add ethanol solution, make the ethanol volume fraction in the mixed solution of enzymolysis solution and ethanol solution be 50%, and perform ultrasonic extraction, the ex...

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PUM

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Abstract

The invention relates to the field of plant extraction and in particular relates to a novel method for extracting peony polysaccharide from peony seed meals. The method for extracting peony polysaccharide from peony seed meals comprises the following steps of crushing, carrying out enzymatic hydrolysis, carrying out ultrasonic extraction with ethanol, carrying out microwave extraction with ethanol, adsorbing with macroporous resin, carrying out enzymatic hydrolysis and removing protein by membrane ultrafiltration, decolorizing, and concentrating and drying to obtain the peony polysaccharide. The method disclosed by the invention has the beneficial effects that the waste peony seed meals are taken as raw materials, peony seed meals is firstly subjected to enzymatic hydrolysis by an enzymatic method, and then an organic solvent such as ethanol is added to extract paeoniflorin, then adsorption is carried out by macroporous resin, and enzymatic hydrolysis is carried out, protein is removed by ultrafiltration membrane and concentrated and dried to obtain the peony polysaccharide of which the yield reaches 12.8%.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a method for extracting polysaccharides from peony seed dregs. Background technique [0002] Nowadays, the research on plant polysaccharides is attracting more and more attention, and the international scientific community even proposes that the 21st century is the century of polysaccharides; scientific experimental studies have shown that many plant polysaccharides have biological activities, including immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-radiation, Antibacterial, antiviral, liver protection and other health care functions. Therefore, plant polysaccharides have long been widely used in the fields of public life such as medicine and catering. [0003] Common plant polysaccharides include tea polysaccharides, wolfberry polysaccharides, konjac mannan, ginkgo biloba polysaccharides, seaweed polysaccharides, lentinan, tremella polysaccharides, ganoderma p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 余洪智毛文岳吴震生刘俊民
Owner HEZE YAO & SHUN PEONY BIOTECH
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