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Bacillus licheniformis synbiotic composition and its preparation

A technology of bacillus licheniformis and its composition, applied in the field of microbiology, can solve the problems of not being able to represent the highest number of viable bacteria, and achieve the effects of quick onset, avoiding side effects, and enhancing the inhibitory effect

Active Publication Date: 2017-05-24
SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because Bacillus licheniformis is a facultative anaerobic bacterium, the results were obtained under the condition of sufficient oxygen, while the intestinal tract is under anaerobic conditions, and the metabolic pathways of bacteria are different under different conditions, so the conclusions disclosed in the patent are not accurate. Must be suitable for Bacillus licheniformis in the intestinal environment
Moreover, the disclosed invention only measures the number of viable bacteria in culture for 24 hours. A large number of tests have proved that the addition of prebiotics will change the growth cycle of the bacteria, and some bacteria have autolyzed in 24 hours. Taking 24 hours as a single sampling point cannot represent bacteria. The highest number of live bacteria

Method used

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  • Bacillus licheniformis synbiotic composition and its preparation
  • Bacillus licheniformis synbiotic composition and its preparation
  • Bacillus licheniformis synbiotic composition and its preparation

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Experimental program
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Effect test

Embodiment 1

[0013] It is proved that effective additive doses of lactulose, stachyose, raffinose, mannose oligosaccharides and resistant dextrin can significantly promote the proliferation of Bacillus licheniformis CMCC63516 under aerobic conditions, prolong the logarithmic growth phase of Bacillus licheniformis, shorten The doubling time of Bacillus licheniformis bacteria in the logarithmic growth phase reduces the autolysis rate of the bacteria. Some of the oligosaccharides have a better effect on the proliferation of Bacillus licheniformis than soybean oligosaccharides.

[0014] Bacterial species: Bacillus licheniformis CMCC63516 (the strain number of China Medical Bacteria Collection Center)

[0015] Seed medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2.

[0016] Basal medium: same as seed medium.

[0017] Oligosaccharide medium (g / 100ml): beef powder 0.5, peptone 1.0, NaCl 0.5, pH7.0-7.2, add different concentrations of oligosaccharides (0.01, 0.05, 0.10, 0.50, 1...

Embodiment 2

[0026] It is proved that effective doses of prebiotics have different effects on promoting the proliferation of Bacillus licheniformis CMCC63516 under aerobic and anaerobic conditions.

[0027] The test results of prebiotics on CMCC63516 proliferation-promoting ability under the condition of table 2 oxygen consumption (viable bacteria number unit: 10 8 CFU / ml)

[0028]

[0029] It can be seen from Table 2 that there are significant differences in the proliferation-promoting effects of various prebiotics on Bacillus licheniformis CMCC63516 under aerobic conditions and anaerobic conditions. Even prebiotics, which have a strong proliferation-promoting effect under aerobic conditions, inhibited CMCC63516 under anaerobic conditions. Under anaerobic conditions, add 0.01%-0.05% lactulose to the medium, the optimal concentration is 0.05%; 0.01%-0.50% stachyose, the optimal concentration is 0.05%; 0.05%-0.10% raffinose, the optimal concentration The optimal concentration is 0.05%; 0...

Embodiment 3

[0031] Shaking tube test was used to confirm the effect of different prebiotics on promoting the ability of Bacillus licheniformis CMCC63516 to inhibit conditional pathogenic Staphylococcus aureus under anaerobic conditions.

[0032] Indicator bacteria: Staphylococcus aureus CMCC (B) 26003 was purchased from China Medical Bacteria Collection Center.

[0033] Staphylococcus aureus culture medium and culture conditions: 0.2% glucose, 1.0% peptone, 0.5% NaCl, 0.5% beef extract, pH 7.0. 37°C, 180r / min shaker culture for 18h, the logarithmic growth phase bacteria solution to make the number of viable bacteria about 10 5 -10 6 CFU / ml, spare.

[0034] Preparation of Bacillus licheniformis CMCC63516 seed solution: same as in Example 1.

[0035] experiment method:

[0036] 1. Seed solution preparation: Aseptically inoculate a ring of slant strains into the liquid medium, culture at 37°C, 180rpm shaker for 16hrs, take the logarithmic growth phase culture solution and dilute it 5 tim...

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Abstract

A Bacillus licheniformis synbiotic composition and its preparation applied in the field of microbial technology, a Bacillus licheniformis synbiotic composition, including Bacillus licheniformis CMCC63516 live bacteria powder, oligosaccharide prebiotics, oligosaccharide prebiotics It is a kind of lactulose, raffinose, stachyose, mannose oligosaccharides, and resistant dextrin. The mass ratio of Bacillus licheniformis CMCC63516 live bacteria powder to oligosaccharide prebiotics is 1:1.25-56, preferably 1: 3‑45; under aerobic and anaerobic conditions, each prebiotic has a proliferative effect on Bacillus licheniformis, and the proliferative effect of each prebiotic on Bacillus licheniformis is related to the amount added in the seed medium. The composition is prepared into oral preparations, common tablets. The invention can significantly promote the proliferation of Bacillus licheniformis CMCC63516 under aerobic and anaerobic conditions, such as lactulose, stachyose, raffinose, oligomannose and resistant dextrin. Yuan can significantly enhance the inhibitory effect of CMCC63516 on Staphylococcus aureus, and has a faster onset and shorter course of treatment in the treatment of intestinal diseases such as diarrhea.

Description

technical field [0001] The invention relates to a bacillus licheniformis synbiotic composition and a preparation thereof in the technical field of microorganisms. Background technique [0002] Microecological preparations are divided into three categories according to the properties of ingredients, probiotics, prebiotics and synbiotics preparations. Among them, synbiotics are a new generation of microecological regulators, and its definition was first proposed by Gibon in 1995. It refers to a kind of microecological product that is used in combination with probiotics and prebiotics. In the combination of synbiotics, prebiotics can not only exert their own beneficial effects by interacting with intestinal bacterial flora, but also prolong the survival time of probiotics, selectively increase the number of probiotics ingested into the host, and make the effect of probiotics more effective. Remarkably durable. The combination of prebiotics and probiotics is not a simple addit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/00A61P1/12
Inventor 雷健吴楠彭勃邢丹何新李雪龙刘心
Owner SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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