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Preparation method and detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization

A detection method and algae toxin technology, applied in Raman scattering, measuring devices, instruments, etc., to achieve high-sensitivity detection

Inactive Publication Date: 2014-08-27
JIANGNAN UNIV
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Problems solved by technology

[0008] At present, the combination of ICA technology and other methods or materials has become a new development trend and research hotspot, but it is still in the preliminary stage to use the characteristics of beacon molecules to enhance Raman on the surface of nanoparticles combined with the principle of ICA reaction for highly sensitive detection.

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  • Preparation method and detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization
  • Preparation method and detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization
  • Preparation method and detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization

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Embodiment Construction

[0028] (1) Preparation of algal toxin monoclonal antibody: After mixing the immunogen of synthetic algal toxin and Freund's adjuvant, mice are stimulated to produce antibodies against algal toxin. After five times of immunization, blood is collected from the tail to detect whether the serum contains algae Antibodies to the toxin. The mice capable of producing algae toxin antibodies were immunized three days later, sacrificed, and the spleen was aseptically removed, and the mouse spleen cells and SP20 cells were fused under the action of PEG, and 7 days later, they were screened by indirect competitive enzyme-linked immunosorbent assay to obtain The cell line capable of secreting algal toxin monoclonal antibody, and then after subcloning three single cells, prepared ascites by in vitro induction method, and purified to obtain algal toxin monoclonal antibody. Such as figure 1 .

[0029] (2) Preparation of spherical and star-shaped nanoparticles:

[0030] Spherical nanoparticl...

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Abstract

The invention discloses a preparation method and a detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization and belongs to the technical field of nanometer materials and biochemical detection. The preparation method comprises the following steps: preparing an algal toxin antibody, preparing spherical and star-like nano particles, preparing a gold-labeled antibody with a Raman signal, assembling a paper-based Raman test strip and application in algal toxin detection. The invention provides a high-sensitivity paper-based Raman visualization method for rapidly detecting the algal toxin. The nano particles with the Raman signal are synthesized, the algal toxin antibody is labeled, and the method is used for performing high-sensitivity detection on the algal toxin by combining the immunogold labeling technique (ICA) with a Raman detection instrument.

Description

technical field [0001] The invention relates to a preparation method and a detection method of high-sensitivity paper for Raman visualization and rapid detection of algal toxins, belonging to the technical field of nanomaterials and biochemical detection. Background technique [0002] Microcystin (MC), or microcystin. Polypeptide toxins isolated from cyanobacteria blooms are water-soluble, heat-resistant and fairly stable, and cannot be destroyed by heating and boiling; coagulation precipitation, filtration, chlorine addition, oxidation, and activated carbon adsorption in tap water treatment processes are also It cannot be completely removed. Microcystin is easily soluble in water, methanol or acetone, non-volatile, and resistant to pH changes. The chemical properties are quite stable, and the natural degradation process is very slow. MC can maintain a stable state for up to 27 days in deionized water, 12 days in sterilized river water, and degrade within 7 days in ordina...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/65
CPCG01N33/558G01N21/658G01N33/54346G01N33/54373
Inventor 徐丽广胥传来邢常瑞匡华马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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