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Application of α-melanocyte-stimulating hormone in the preparation of drugs for preventing radiation damage by increasing white blood cells

A technology to increase white blood cells and melanocytes, applied in drug combinations, extracellular fluid diseases, pharmaceutical formulations, etc., can solve problems such as the protective effect of ionizing radiation, and achieve the effect of improving the survival rate

Inactive Publication Date: 2016-01-13
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it has a protective effect on ionizing radiation has not yet been reported.

Method used

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  • Application of α-melanocyte-stimulating hormone in the preparation of drugs for preventing radiation damage by increasing white blood cells
  • Application of α-melanocyte-stimulating hormone in the preparation of drugs for preventing radiation damage by increasing white blood cells
  • Application of α-melanocyte-stimulating hormone in the preparation of drugs for preventing radiation damage by increasing white blood cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In vivo experiments in mice

[0020] 1. The purpose of the experiment To clarify the preventive effect of mouse melanocyte stimulating hormone (α-MSH) on radiation injury in mice.

[0021] 2. Experimental animal experiments BALB / C mice, C57BL / 6 mice and ICR mice were selected for experiments, adult male mice with a body weight of 22±1g.

[0022] 3. Irradiation Treatment The mice were irradiated for one time, the irradiation dose was 7.0Gy for BALB / C mice, 7.2Gy for C57BL / 6 mice, and 7.5Gy for ICR mice. The three kinds of mice were tested for immune indicators such as peripheral blood and organ indexes on the 9th day after irradiation, and the ICR mice were tested for 30-day survival rate.

[0023] 4. Experimental grouping Experiments revolve around three types of in vivo experiments on mice and some in vitro experiments. The experiments include normal group and radiation control group; mouse melanocyte stimulating hormone (α-MSH) administration group; mouse melanocyte ...

Embodiment 2

[0059] In vitro experiments

[0060] 1. In vitro cytology experiments to explore the protective mechanism of α-MSH radiation damage.

[0061] 2. Experimental materials: Spleen and bone tibia of normal C57 mice were taken, splenocytes and bone marrow nucleated cells were separated by ficoll, and 2×10 6 The cell suspension was plated in a 96-well plate.

[0062] 3. Experimental method: Add α-MSH drug for incubation 30 minutes before irradiation. After irradiation, 37°C, 5% CO 2 After culturing in the incubator for 16 hours, the cell viability was detected by bioluminescent method.

[0063] 4. Statistical analysis: All data processing was analyzed with SPSS version 16.0 software package, and the test results were based on ±s means that after the Levene test, the LSD method was used to compare the differences between the groups, and P<0.05 was considered statistically significant.

[0064] 5. Experimental results: The results of this experiment show that melanocyte-stimulatin...

Embodiment 3

[0066] Preparation of Injections

[0067] Take 10g of melanocyte-stimulating hormone, add it to 440ml of water for injection in which 5g of sodium lauryl sulfate has been dissolved, add 2g of sodium thiosulfate and stir to dissolve, add sodium bicarbonate to adjust the pH to 5.5-8.5, add 5g of activated carbon, and let it cool at room temperature. Stir and absorb for 30 minutes, remove charcoal, add water to 500ml, fine filter, fill with 5ml each, and sterilize to obtain melanocyte stimulating hormone injection.

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PUM

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Abstract

The invention discloses α - Application of melanocyte-stimulating hormone in preparing medicine for treating elevated white blood cells and preventing radiation damage. The prevention of radiation damage refers to: melanocyte-stimulating hormone promotes the proliferation of bone marrow and spleen cells. The elevated leukocytes refers to leukopenia caused after chemotherapy. The prophylactic administration of the present invention can effectively improve the 30-day survival rate of mice irradiated with a lethal dose. Simultaneous prophylactic administration has obvious protective effect on chemotherapy-induced leukopenia and thrombocytopenia without obvious toxic and side effects.

Description

technical field [0001] The invention belongs to the expansion of the application field of medicine, and relates to the application of α-melanocyte stimulating hormone (α-MSH) in the fields of increasing white blood cells and protecting against radiation damage. Background technique [0002] α-Melanocyte-stimulating hormone (α-MSH) is an endogenous neuroimmune regulatory peptide, and its precursor, proopiomelanocortin (POMC), was first detected in the pituitary gland. α-MSH consists of 13 amino acids, its amino acid sequence is: Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Vol, and the 3 amino acids at the C-terminal are Lys- Pro-Vol is the signal sequence for α-MSH to exert biological effects. α-MSH binds to melanocortin receptor (MCR) to play a biological role, has strong antipyretic, anti-inflammatory and immune regulation effects, and has obvious inhibitory effect on some early inflammatory factors. Many evidences show that the concentration of α-MSH is increased in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/10A61P7/00A61P39/00
Inventor 吴红英龙伟樊赛军周则卫沈秀李德冠靳瑾徐畅张俊伶孟爱民闫玉军王小春刘鉴峰褚丽萍杨翠红杜利清王彦
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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