Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel metalloprotein and process for producing same, and prophylactic or therapeutic agent for corneal and conjunctival diseases comprising said metalloprotein

A manufacturing method and protein technology, which is applied to the preparation method of peptides, peptide/protein components, animal/human proteins, etc., can solve the problems such as selenium-lactoferrin has no discovery effect, achieve high therapeutic effect and safety, and alleviate Effect of side effects, high therapeutic effect

Inactive Publication Date: 2014-08-20
KEIO UNIV +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, in general, the iron-binding property of the iron-binding pocket present in lactoferrin is about 2000 times higher than that of other cations, and it is considered to be specific for iron (Non-Patent Document 4). No known metalloproteins that replace iron in lactoferrin with other metals
Moreover, it is difficult to infer the effect of the selenium-lactoferrin of the present invention on eye diseases, and no report of its effect has actually been found.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel metalloprotein and process for producing same, and prophylactic or therapeutic agent for corneal and conjunctival diseases comprising said metalloprotein
  • Novel metalloprotein and process for producing same, and prophylactic or therapeutic agent for corneal and conjunctival diseases comprising said metalloprotein
  • Novel metalloprotein and process for producing same, and prophylactic or therapeutic agent for corneal and conjunctival diseases comprising said metalloprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Preparation of selenium-lactoferrin

[0077] To an aqueous solution containing 5.4 mg of selenium (I) chloride (manufactured by Wako Pure Chemical Industries, Ltd.), add 1 g of apolactoferrin (manufactured by Appwell Co., Ltd.), adjust the liquid volume to 10 mL, and confirm that the pH of the aqueous solution is 3 or more, and stirred at 25° C. using a rotor at 240 beats / min for 30 minutes to bind selenium to apolactoferrin. Next, transfer the mixed solution of selenium (I) chloride and apolactoferrin to a dialysis cellulose tube (molecular weight cut-off 12,000-14,000; manufactured by Asswan Co., Ltd.), and use 6 L of pure water (17 MΩ or more ), exchanged pure water every 12 hours, and at the same time performed dialysis at 4°C for 48 hours to remove excess selenium that was not bound to apolactoferrin. After dialysis, freeze-drying was performed using a freeze dryer FDU-12AS (manufactured by Asswan Co., Ltd.).

Embodiment 2

[0079] The procedure of Example 1 was repeated except that lactoferrin (manufactured by Tatsua Japan Co., Ltd.) was used as a raw material.

Embodiment 3

[0081] The procedure of Example 1 was repeated, and in this example, excess selenium was removed in the following order instead of dialysis. That is, in this example, a UF membrane (Microza AP-0013 (UF) with a molecular weight cut-off of 6,000; manufactured by Asahi Kasei Co., Ltd.) was installed in a pencil-shaped modular bench-top filter device (Microza UF·M FPS-24001; Asahi Kasei Co., Ltd. system) on. Furthermore, 10 mL of a mixed solution of selenium (I) chloride and apolactoferrin was ultrafiltered three times at room temperature (above 20° C.) using the device (operating pressure: module outlet pressure of 40 kPa).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides: a novel metalloprotein, i.e., selenium-lactoferrin; and a process for producing the metalloprotein. Selenium-lactoferrin according to the present invention can be produced suitably by adding a selenium salt to a solution containing lactoferrin and / or apolactoferrin and then subjecting the resultant mixed solution to dialysis or ultrafiltration. Selenium-lactoferrin according to the present invention has an excellent therapeutic effect on corneal and conjunctival diseases, and is suitable for mass production on an industrial scale.

Description

technical field [0001] The present invention relates to a novel metalloprotein, a method for producing the same, and a prophylactic or therapeutic agent for keratoconjunctival diseases containing the metalloprotein. More specifically, the present invention relates to a protein comprising selenium and apolactoferrin, a protein in which selenium is bound to apolactoferrin, ie, selenium-lactoferrin, and a method for producing the same, and corneal and conjunctival diseases containing said metalloprotein preventive or therapeutic agents. The selenium-lactoferrin of the present invention is useful for the prevention or treatment of corneal and conjunctival diseases such as dry eye, keratoconjunctivitis sicca, superficial punctate keratopathy, corneal erosion, or corneal ulcer. Background technique [0002] The cornea is a thin, transparent tissue with a thickness of about 1 mm and has no blood vessels. It has a very regular and fine structure consisting of an epithelial layer, B...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/79C07K1/02C07K1/34A61K38/16A61P27/02
CPCA61K9/0048A61K38/40C07K14/79A61P27/02C07K1/02C07K1/34A61K38/16
Inventor 樋口明弘井上浩义坪田一男
Owner KEIO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com