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A method for the detection of microcystin-lr with palladium-gold alloy nano-cage immunochromatographic test strips

A technology of immunochromatographic test paper and microcystin, which is applied in the field of immunoassay chemistry, can solve the problem of unsuitable preservation of gold nanoparticle sol, and achieve the effect of stabilizing the conjugate

Active Publication Date: 2015-08-26
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to overcome the shortcoming that gold nanoparticle sol, a commonly used colored marker, is not suitable for preservation, and provide a method with better technical performance than the Chinese patent application with application number 201310610348.8, that is, a palladium-gold alloy nanocage immunochromatography Method for detecting microcystin-LR with test strip

Method used

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  • A method for the detection of microcystin-lr with palladium-gold alloy nano-cage immunochromatographic test strips
  • A method for the detection of microcystin-lr with palladium-gold alloy nano-cage immunochromatographic test strips
  • A method for the detection of microcystin-lr with palladium-gold alloy nano-cage immunochromatographic test strips

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Embodiment

[0024] Embodiment, its steps are:

[0025] 1 Preparation of palladium-gold alloy nanocage Pd-Au NCs

[0026] Palladium-gold alloy nanocages were prepared as follows: Gold nanocages were prepared with reference to the modified method of Xia et al [Lim B.K., Li Z.Y., Xia Y., et.al.Synthesis of Pd-Au bimetallic nanocrystals via controlled overgrowth( J).J.Am.Chem.Soc.2010,132(8):2506-2507.]. Mix 55mL PVP (MW=38000), 300mgL-ascorbic acid, 1500mg KBr and 285mg chloropalladium acid and heat to 80°C (to boiling), react under constant stirring for 3 hours, after cooling to room temperature, centrifuge the product, and use Hydro-ethanol and deionized water were rinsed three to five times to remove dissolved bromide. The resulting product is sealed and preserved as a seed for the next step of synthesis.

[0027] The Pd nanocages synthesized in the above step are used as crystal seeds to synthesize Pd-Au nanocage particles. Take 20ml of deionized water, add 0.5ml of concentrated Pd n...

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Abstract

The invention relates to a method for detecting microcystin-LR through a palladium-gold alloy nanoscale cage immune chromatography test strip, and belongs to the technical field of immunity chromatography chemistry. The method comprises the following steps: preparing a Pd-Au bimetal nanoscale cage with a hollow structure by taking Pd nano-particles as seed crystals; spraying a monoclonal antibody for labeling the microcystin-LR to a combined pad; spraying BSA (Bovine Serum Albumin) coupling microcystin-LR on a detection line; spraying goat anti-rat IgG on a control line to prepare the immune chromatography test strip for fast detecting the microcystin-LR in a water body; and carrying out quantitative analysis on the microcystin-LR contained in a test sample by a competition immune chromatography and by reading the grey value of gold nanoscale cage-microcystin antibody mixture remained on the detection line. According to the method, because the adhesion of sulfhydryl and amido in a protein molecule of a Pd-Au alloy is higher than the binding force of a single-metal gold nanoscale cage by virtue of the synergistic action of two metal elements in the alloy, a microcystin antibody is easier to label by that Pd-Au alloy nanoscale cage than by the gold nanoscale cage, and the formed Pd-Au alloy nanoscale cage-microcystin antibody mixture is more stable.

Description

technical field [0001] The invention belongs to the technical field of immunoanalysis chemistry. Background technique [0002] For freshwater lakes with serious eutrophication, the temperature rise every summer will cause blue-green algae to bloom and cause serious pollution. In the large family of cyanobacteria, some species can secrete strong toxins [see Shi Hongxing, Qu Jiuhui, Wang Aimin, etc., Preliminary Research on Blue-green Algae and Algal Toxins in Guanting Reservoir, Chemical Journal of Chinese Universities, 2005, 26(9): 1650-1652], and microcystins (microcystins, MCs) are typical representatives. Among them, MCs-LR containing leucine and arginine at positions 2 and 4 is the most likely and most toxic cyclic heptapeptide hepatotoxin known, and is a cancer-promoting factor in freshwater algae [see Yu S.Z. , Chen G., Zhi X.L., et al.Primary liver cancer: natural toxins and prevention in China(J).Toxicol Sci,1998,23(Suppl2):143-148; and see Yu Shunzhang, Zhao Ning, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
CPCG01N33/54393G01N33/587
Inventor 杨云慧王勤牛司朋唐赛赛尹寒蕊崔光鑫黄贵春郑丽
Owner YUNNAN NORMAL UNIV
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