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Method for detecting microcystin-LR through palladium-gold alloy nanoscale cage immune chromatography test strip

A technique of immunochromatographic test paper and microcystin, which is applied in the field of immunoassay chemistry, can solve the problem that gold nanoparticle sol is not suitable for storage, and achieves the effect of stabilizing the conjugate

Active Publication Date: 2014-08-20
YUNNAN NORMAL UNIV
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Problems solved by technology

[0008] The purpose of the present invention is to overcome the shortcoming that gold nanoparticle sol, a commonly used colored marker, is not suitable for preservation, and provide a method with better technical performance than the Chinese patent application with application number 201310610348.8, that is, a palladium-gold alloy nanocage immunochromatography Method for detecting microcystin-LR with test strip

Method used

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  • Method for detecting microcystin-LR through palladium-gold alloy nanoscale cage immune chromatography test strip
  • Method for detecting microcystin-LR through palladium-gold alloy nanoscale cage immune chromatography test strip
  • Method for detecting microcystin-LR through palladium-gold alloy nanoscale cage immune chromatography test strip

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Embodiment

[0024] In an embodiment, the steps are:

[0025] 1 Preparation of palladium-gold alloy nanocage Pd-Au NCs

[0026] The palladium-gold alloy nanocages are prepared according to the following method: the gold nanocages are prepared according to the modified method of Xia et al. [Lim BK, Li ZY, Xia Y., et.al. Synthesis of Pd-Au bimetallic nanocrystals via controlled overgrowth( J). J. Am. Chem. Soc. 2010, 132(8): 2506-2507.]. Mix 55 mL of PVP (MW=38000), 300 mg of L-ascorbic acid, 1500 mg of KBr, and 285 mg of chloropalladium acid and heat to 80°C (to boiling), and react for 3 hours under constant stirring. After cooling to room temperature, the product is separated by centrifugation. Water ethanol and deionized water are repeatedly washed three to five times to remove dissolved bromide. The resulting product is sealed and stored as a seed for the next step of synthesis.

[0027] The Pd nanocage synthesized in the above step is used as a crystal seed to synthesize Pd-Au nanocage part...

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Abstract

The invention relates to a method for detecting microcystin-LR through a palladium-gold alloy nanoscale cage immune chromatography test strip, and belongs to the technical field of immunity chromatography chemistry. The method comprises the following steps: preparing a Pd-Au bimetal nanoscale cage with a hollow structure by taking Pd nano-particles as seed crystals; spraying a monoclonal antibody for labeling the microcystin-LR to a combined pad; spraying BSA (Bovine Serum Albumin) coupling microcystin-LR on a detection line; spraying goat anti-rat IgG on a control line to prepare the immune chromatography test strip for fast detecting the microcystin-LR in a water body; and carrying out quantitative analysis on the microcystin-LR contained in a test sample by a competition immune chromatography and by reading the grey value of gold nanoscale cage-microcystin antibody mixture remained on the detection line. According to the method, because the adhesion of sulfhydryl and amido in a protein molecule of a Pd-Au alloy is higher than the binding force of a single-metal gold nanoscale cage by virtue of the synergistic action of two metal elements in the alloy, a microcystin antibody is easier to label by that Pd-Au alloy nanoscale cage than by the gold nanoscale cage, and the formed Pd-Au alloy nanoscale cage-microcystin antibody mixture is more stable.

Description

Technical field [0001] The invention belongs to the technical field of immunoanalytical chemistry. Background technique [0002] Freshwater lakes with serious eutrophication will cause blue-green algae outbreaks and serious pollution every summer when the temperature rises. In the large family of cyanobacteria, there are some species that secrete strong toxins [see Shi Hongxing, Qu Jiuhui, Wang Aimin, etc., Guanting Reservoir Blue-green Algae and Preliminary Study on Phytotoxins, Chemical Journal of Chinese Universities, 2005, 26(9): 1650-1652], and microcystins (MCs) is a typical representative. Among them, MCs-LR containing leucine and arginine at positions 2, 4 is the most prone and most toxic cyclic heptapeptide hepatoxin known, and is a cancer-promoting factor in freshwater algae [see Yu SZ ,Chen G.,Zhi XL,et al.Primary liver cancer:natural toxins and prevention in China(J).Toxicol Sci,1998,23(Suppl2):143-148; and see Yu Shunzhang, Zhao Ning, Zi Xiaolin, etc. , Study on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/54393G01N33/587
Inventor 杨云慧王勤牛司朋唐赛赛尹寒蕊崔光鑫黄贵春郑丽
Owner YUNNAN NORMAL UNIV
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