In-vitro culture medium of mycoplasma ovipneumoniae and preparation method thereof

Abstract

The invention discloses an in-vitro culture medium of mycoplasma ovipneumoniae and a preparation method thereof. The preparation method comprises the steps of mixing tryptone, soy peptone, sodium chloride, lactose, a yeast leaching agent and phenol red, then sterilizing under high pressure, cooling the mixture to below 37 DEG C, then adding 100-200mL / L of deactivated healthy porcine serum and 200 thousand U / L of penicillin dissolved by sterilizing water into the mixture, and regulating the pH value to be 7.4-7.8 with NaOH, so as to obtain the culture medium. Being cultivated by the culture medium, the mycoplasma ovipneumoniae can grow only in 24 hours, the growing titer can reach 10<-10>ccu, and compared with the prior art, the culture medium has simpler composition and lower cost.

Description

technical field [0001] The invention relates to a bacterial culture medium and a preparation method thereof. Specifically, the invention relates to a culture medium for Mycoplasma pneumoniae in vitro and a preparation method for the culture medium. Background technique [0002] Mycoplasma ovis pneumoniae ( Mycoplasma ovipneumoniae , Mo) is a prokaryotic microorganism without a cell wall structure between bacteria and viruses, mainly causing proliferative interstitial pneumonia in sheep, goats, especially lambs. The pathogen was first isolated from the lungs of sheep with lung adenocarcinoma by Mackay et al. (1963), and subsequently Carmichael (1972) named it Mycoplasma ovis pneumoniae. Since then, the pathogen has been reported in many countries and regions, such as New Zealand, Hungary, Iceland, the United Kingdom, Australia, Spain, Turkey, many countries in Africa and West Asia (Jonas et al, 1991; Linvingston et al, 1979), The development of the sheep industry has cause...

AI Technical Summary

Problems solved by technology

In addition to basic nutrition, the artificial medium used at this stage also needs ingredients such as beef heart infusion, yeast liquid, coenzyme I, amino acids, etc. In addition, 10% to 20% of animal serum and other ingredients need to be added. Its configuration is complicated, the process is cumbersome, and the cost is high (reference: Wu Yimou, Ye Yuankang, 2nd edition of Mycoplasmaology). Although it has been improved many times, an improved KM2 medium has been formed, which reduces the many processes