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Preparation method for glucan bioseparation and purification medium

A technology for biological separation and glucan, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve the problems of difficulty in obtaining high porosity data, complex reaction process and high preparation cost, To increase the static adsorption capacity, the preparation process is simple and stable, and the uniformity is improved.

Active Publication Date: 2014-08-20
WUHAN HUIYAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method of dextran bio-separation and purification medium, which overcomes the complexity of the reaction process in the prior art, too many influencing factors, and the difficulty in obtaining high-porosity data and high particle size uniformity. Sugar separation and purification medium, and the preparation cost is relatively high

Method used

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  • Preparation method for glucan bioseparation and purification medium
  • Preparation method for glucan bioseparation and purification medium
  • Preparation method for glucan bioseparation and purification medium

Examples

Experimental program
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Embodiment 1

[0037] Weigh 3000g of dextran into 4000g of deionized water, heat to 50°C, stir and dissolve at 400rpm for 20h, add 250g of 50% NaOH solution, stir at 500rpm for 1h; weigh 70g of cellulose acetate into 6000g of dichloroethylene in alkanes, heated to 50°C, stirred and dissolved at 500rpm for 20h, quickly added the dextran solution into dichloroethane, weighed 600g of epichlorohydrin solution, slowly added dropwise to dichloroethane for 3h, and heated to 50 ℃, stirred vigorously at 900rpm for 3 hours, took samples and put them into ice water at 4℃ to solidify and form, and tested the particle size, appearance roundness and pore size of the dextran medium under a particle size analyzer and a digital microscope, respectively. Stop heating, reduce the rotation speed to 200rpm, use 4°C ice water on the outer wall of the oil phase reactor to quickly cool down to below 23°C, transfer the dextran medium to a microporous glass funnel, filter with a vacuum pump, and clean 10-20 liters res...

Embodiment 2

[0040] Weigh 1500g of dextran and add it to 3000g of deionized water, heat to 50°C, stir and dissolve at 400rpm for 20h, add 250g of 50% NaOH solution, and stir at 500rpm for 1h; weigh 70g of cellulose acetate and add it to 3500g of dichloroethylene In alkanes, heated to 50°C, stirred and dissolved at 500rpm for 20h, quickly added the dextran solution into dichloroethane, weighed 350g of epichlorohydrin solution, slowly added dropwise to dichloroethane for 3h, and heated to 50 ℃, stirred vigorously at 900rpm for 3 hours, took samples and put them into ice water at 4℃ to solidify and form, and tested the particle size, appearance roundness and pore size of the dextran medium under a particle size analyzer and a digital microscope, respectively. Stop heating, reduce the rotation speed to 200rpm, use 4°C ice water on the outer wall of the oil phase reactor to quickly cool down to below 23°C, transfer the dextran medium to a microporous glass funnel, filter with a vacuum pump, and ...

Embodiment 3

[0043] Weigh 100g of dextran and add it to 200g of water, heat to 50°C, stir and dissolve at 400rpm for 20h, add 250g of 50% NaOH solution, and stir at 500rpm for 1h; weigh 70g of cellulose acetate and add it to 1000g of dichloroethane , heated to 50°C, stirred and dissolved at 500rpm for 20h, quickly added the dextran solution into dichloroethane, weighed 100g of epichlorohydrin solution and slowly added dropwise to dichloroethane for 3h, heated to 50°C, Stir vigorously at 900rpm for 3 hours, take samples and put them into ice water at 4°C to solidify and form, and measure the particle size, appearance roundness and pore size of the dextran medium under a particle size analyzer and a digital microscope, respectively. Stop heating, reduce the rotation speed to 200rpm, use 4°C ice water on the outer wall of the oil phase reactor to quickly cool down to below 23°C, transfer the dextran medium to a microporous glass funnel, filter with a vacuum pump, and clean 10-20 liters respect...

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Abstract

The invention relates to a preparation method for a glucan bioseparation and purification medium. The preparation method comprises the following steps: adding glucan into water, carrying out heating, stirring and dissolving and then adding an aqueous sodium hydroxide solution so as to obtain an alkaline glucan solution; adding a cross-linking agent into an oil phase and carrying out heating, stirring and dissolving; and rapidly adding the alkaline glucan solution into the oil phase, carrying out heating and violent stirring so as to obtain emulsion droplets with a uniform particle size of 20 to 120 mu m, slowly adding chloropropylene oxide into the emulsion for a reaction, carrying out rapid cooling and then carrying out cleaning with toluene and methanol so as to obtain the glucan bioseparation and purification medium. The method only needs one step of reaction, substantially reduces influence factors, has easily controllable reaction process, improves porosity of the medium and uniformity of granularity, enhances static adsorption capacity and accelerates a mass transfer rate, thereby improving purification and separation efficiency; moreover, the preparation method for the medium is simple and stable and suitable for large scale production. Raw materials used for preparation of the medium are fewer and cheaper, so production cost is greatly reduced.

Description

technical field [0001] The invention relates to a preparation method of a dextran biological separation and purification medium, belonging to the field of biological separation and purification. Background technique [0002] At present, in the field of bio-separation and purification, bio-separation and purification media rely heavily on foreign imports, but my country's bio-industry is facing the embarrassing situation of being controlled by foreign manufacturers, which means that the lifeline of my country's strategic bio-industry is in the hands of other countries. Therefore, it is necessary and urgent to develop industrialized bioseparation and purification technology and products. In the separation and purification technology, the medium is the fundamental factor affecting the purification and separation results. The optimal design of media usually needs to take into account static adsorption capacity, mass transfer rate and flow characteristics. However, in many cases...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/28B01J20/30
Inventor 易国军
Owner WUHAN HUIYAN BIOTECH
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