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Method for extracting protein from soybean meal by adopting ultrasonic-assisted reverse micelle extraction process

An ultrasound-assisted, reversed micellar technology, applied in the field of plant protein extraction and processing, can solve the problem that the extraction rate of reversed micelles is not particularly ideal, and achieve the effect of low degree of denaturation, good functionality and high purity

Inactive Publication Date: 2014-08-20
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, liquid-liquid extraction is mainly used to extract proteins. Because there is a micro-water pool in the reverse micelles, it can dissolve amino acids, peptides and proteins and other biologically active molecules without inactivating them, but the extraction rate of reverse micelles is not special. ideal

Method used

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  • Method for extracting protein from soybean meal by adopting ultrasonic-assisted reverse micelle extraction process

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Experimental program
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Effect test

Embodiment 1

[0018] The defatted soybean meal was crushed and sieved, mixed with water to form a mixed solution, and 2% cellulase was added to the mixed solution at an enzymolysis temperature of 50°C and an enzymolysis pH of 5.5 for enzymolysis for 1.5 hours, and then at the enzymolysis temperature Add 3% alkaline protease to the mixture at 60°C and pH 9 for enzymolysis for 2 hours. After enzymolysis, centrifuge to obtain the enzymolysis solution and precipitate. Surfactant AOT with a concentration of 0.06g / mL was mixed with isooctane to form an AOT / isooctane reverse micelle system, and the AOT / isooctane reverse micelle system was added to the enzymolysis solution, and the ultrasonic power was 250W, extraction Ultrasonic-assisted reverse micellar extraction was carried out at a temperature of 50°C and an extraction pH of 7 for 1 hour to obtain an extract. At a stripping temperature of 40°C and a stripping pH of 9, an equal volume of 1 mol / L KCl was added to the extract The solution was bac...

Embodiment 2

[0020] The defatted soybean meal was crushed and sieved, mixed with water to form a mixed solution, and 2% cellulase was added to the mixed solution at an enzymolysis temperature of 50°C and an enzymolysis pH of 5.5 for enzymolysis for 1.5 hours, and then at the enzymolysis temperature Add 3% alkaline protease to the mixture at 60°C and pH 9 for enzymolysis for 2 hours. After enzymolysis, centrifuge to obtain the enzymolysis solution and precipitate. Surfactant AOT with a concentration of 0.08g / mL was mixed with isooctane to form an AOT / isooctane reverse micelle system, and the AOT / isooctane reverse micelle system was added to the enzymatic hydrolysis solution, and the ultrasonic power was 300W, extraction The temperature is 60°C, the extraction pH is 8, and the ultrasonic-assisted reverse micellar extraction is carried out for 1.5h to obtain the extract. The KCl solution was back-extracted for 40 minutes. After the back-extraction, the organic phase and the aqueous phase were...

Embodiment 3

[0022] The defatted soybean meal was crushed and sieved, mixed with water to form a mixed solution, and 2% cellulase was added to the mixed solution at an enzymolysis temperature of 50°C and an enzymolysis pH of 5.5 for enzymolysis for 1.5 hours, and then at the enzymolysis temperature Add 3% alkaline protease to the mixture at 60°C and pH 9 for enzymolysis for 2 hours. After enzymolysis, centrifuge to obtain the enzymolysis solution and precipitate. Surfactant AOT with a concentration of 0.06g / mL was mixed with isooctane to form an AOT / isooctane reverse micelle system, and the AOT / isooctane reverse micelle system was added to the enzymolysis solution, and the ultrasonic power was 300W, extraction Ultrasonic-assisted reverse micellar extraction was carried out at a temperature of 55°C and an extraction pH of 9 for 1 hour to obtain an extract. At a stripping temperature of 35°C and a stripping pH of 8, an equal volume of 1 mol / L KCl was added to the extract The solution was bac...

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Abstract

The invention discloses a method for extracting protein from soybean meal by adopting an ultrasonic-assisted reverse micelle extraction process, and belongs to plant protein extraction and processing technologies. The method comprises the following steps: (1) grinding defatted soybean meal, screening, mixing with water to form a mixed solution, adding cellulase and protease into the mixed solution in sequence, performing step-by-step enzymatic hydrolysis, and after the enzymatic hydrolysis, performing centrifugal separation to obtain enzymatic hydrolysis liquid and deposits; (2) adding a reverse micelle system into the enzymatic hydrolysis liquid for performing ultrasonic-assisted reverse micelle extraction to obtain extracting liquid, performing back extraction on the extracting liquid, and performing the centrifugal separation to obtain an organic phase and a water phase; (3) performing alkali-solution and acid-isolation on the water phase to obtain deposits, adding the water into the deposits for redissolving, ultra-filtering, concentrating in vacuum and freeze-drying to obtain the soybean protein. According to the method, required process equipment is simple and safe to operate; an ultrasonic technology and a reverse micelle extraction technology are combined for extracting the protein from the soybean meal, so that the protein extraction rate is increased and the extraction cost is reduced; the prepared soybean protein is high in purity, low in modification degree and good in functionality.

Description

technical field [0001] The invention belongs to the extraction and processing technology of plant protein, and mainly relates to a method for extracting protein in soybean meal assisted by ultrasonic waves. Background technique [0002] Soybean meal generally contains protein, polysaccharide, fiber and other nutrients, especially rich in protein, which is the most valuable ingredient. Soybean meal protein contains a large number of essential amino acids required by the human body, so it is also an extremely important source of food protein. However, most soybean meal is used as feed, which causes a great waste of soybean protein resources. [0003] The research on protein extraction from reverse micelles began in the 1970s and has been developed for more than 30 years. It is generally believed that the mechanism of protein extraction by reverse micelles is that water-soluble proteins dissolve in the water pool of the reverse micelles system. The reverse micellar system is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14
Inventor 江连洲李杨隋晓楠齐宝坤王中江冯红霞王欢马文君丁简王瑞赵城彬
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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