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Method for extracting moringa flavonoids from moringa leaves

A technology of moringa flavonoids and moringa leaves, applied in directions such as organic chemistry, can solve problems such as no extraction of biologically active substances, and achieve the effects of reducing the amount of impurities introduced, the extraction rate is high, and the extraction time is short

Active Publication Date: 2016-01-27
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it does not involve the extraction of bioactive substances such as flavonoids in Moringa

Method used

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  • Method for extracting moringa flavonoids from moringa leaves
  • Method for extracting moringa flavonoids from moringa leaves
  • Method for extracting moringa flavonoids from moringa leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take 1 kg of Moringa leaves, crush them through a 700-mesh sieve, put them into a filter frame, put them into a CBE-5L type subcritical extraction kettle, seal the extraction kettle, adjust the vacuum in the extraction kettle to 0.1Mpa, add methanol extractant, and adjust the solid-liquid The ratio is 1:20 (g:ml), the extraction temperature is adjusted to 200°C, the extraction pressure is 4.048MPa, and the extraction is carried out 3 times, each extraction is 4h, the extract is put into a solvent recovery kettle, the pressure is reduced to a vacuum of 0.1Mpa to recover the solvent, and vacuum drying , to obtain black-green extract. Add 5 times the amount (by mass) of n-butanol saturated with water for extraction in the extract; use a centrifuge to centrifuge and collect the extract phase; use a rotary evaporator to decompress the extract phase when the vacuum degree is 0.01 to 0.1Mpa. Concentrate to a brownish-yellow extract. Take the extract on a D101 macroporous resi...

Embodiment 2

[0038]Get Moringa leaf 0.9kg, pulverize through 100 mesh sieves, put into filter frame, drop into subcritical extraction kettle, airtight extraction kettle, adjust the vacuum in the extraction kettle to 0.06Mpa, add ethanol extractant, adjust solid-liquid ratio to be 1 :5 (g:ml), adjust the extraction temperature to 80°C, the extraction pressure to 0.108MPa, extract 2 times, extract 0.5h each time, put the extract into the solvent recovery kettle, reduce the pressure to a vacuum of 0.01Mpa to recover the solvent, and dry it in vacuum to obtain Black-green extract. Add 5 times the amount (by mass) of n-butanol saturated with water for extraction in the extract; use a centrifuge to centrifuge and collect the extract phase; use a rotary evaporator to decompress the extract phase when the vacuum degree is 0.01 to 0.1Mpa. Concentrate to a brownish-yellow extract. Take the extract and put it on the AB-8 macroporous resin column, use the ethanol-water and absolute ethanol eluent wit...

Embodiment 3

[0040] Get Moringa leaf 1.3kg, crush through a 500-mesh sieve, put into a filter frame, put into a subcritical extraction kettle, seal the extraction kettle, adjust the vacuum in the extraction kettle to 0.08Mpa, add ethanol extractant, and adjust the solid-liquid ratio to 1 :10 (g:ml), adjust the extraction temperature to 100°C, the extraction pressure to 0.223MPa, extract 3 times, extract 2h each time, put the extract into the solvent recovery kettle, reduce the pressure to a vacuum of 0.1Mpa, recover the solvent, and dry in vacuum to obtain Black-green extract. Add 5 times the amount (by mass) of n-butanol saturated with water for extraction in the extract; use a centrifuge to centrifuge and collect the extract phase; use a rotary evaporator to decompress the extract phase when the vacuum degree is 0.01 to 0.1Mpa. Concentrate to a brownish-yellow extract. Take the extract on AB-8 macroporous resin column, use methanol-water and anhydrous methanol eluent with a volume ratio...

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Abstract

The invention relates to a method for extracting moringa oleifera lam flavone from moringa oleifera lam leaves. The method comprises the steps: 1) drying the moringa oleifera lam leaves, loading into a filter basket, putting into a sub-critical extraction kettle, and closing airtightly the extraction kettle; then adding an organic extraction agent, heating up until an extracted liquid in the sub-critical extraction kettle is in a sub-critical state; and extracting to obtain the extracted liquid, putting into a solvent recycling kettle, carrying out reduced-pressure recycling of the solvent, and thus obtaining a black green extract; 2) adding n-butanol saturated by water into the extract, and extracting; centrifuging by using a centrifuge, and collecting the extracted phase; and carrying out reduced-pressure concentration of the extracted phase by a rotary evaporator to obtain a brown-yellow extract; and 3) loading the brown-yellow extract on a macroporous resin column, then carrying out gradient elution with elution liquids according to an order from small elution liquid polarity to large elution liquid polarity, detecting, taking a fraction having the flavone content of more than 97%, concentrating to a dried powder, recrystallizing with methanol or ethanol, and thus obtaining a flavone product with the purity of more than 98%. The product has less impurity content and high biological activity, and the sub-critical fluid extraction technology has the advantages of high extraction efficiency, short operating period, energy saving, time saving, and high recovery rate of the extraction agent.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products, relates to an extraction method of flavonoids of biologically active substances in Moringa oleifera, relates to a subcritical extraction technology, and is a method for finely separating biologically active substances in Moringa oleifera. Background technique [0002] Moringa oleifera (Moringa oleifera Lam), belonging to the Moringa family of the cauliflower order, is also known as the drumstick tree and the immortal tree. It is native to the sub-Himalayan region of North India and Africa, and is widely distributed in India, Pakistan and other countries. Moringa is rich in nutrients. The calcium contained in Moringa is four times that of milk, potassium is three times that of bananas, iron is three times that of spinach, vitamin C is seven times that of oranges, and vitamin A is four times that of carrots. Moringa leaves and fruit pods contain a variety of minerals, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 丁辉刘骋
Owner TIANJIN UNIV
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