A domestic garbage and sludge decomposing bacterial agent and its application and prepared decomposing substrate
A domestic waste and substrate technology, applied in the direction of application, fungicide, biocide, etc., can solve the problems of low combustion value of domestic waste, threats to people and the environment, and low energy conversion rate, so as to improve crop quality and reduce chemical fertilizers Usage amount, effect of promoting soil remediation
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Embodiment 1
[0039] Preparation of media and strains
[0040] 1. Preparation of culture medium
[0041] 1. Potato and dextrose agar medium (PDA)
[0042] Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.
[0043] 2. Nutrient gravy agar
[0044] It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).
[0045] 3. Gao's Synthetic Medium No. 1
[0046] KNO 3 1g, K 2 HPO 4 0.5g, NaCl 0.5g, FeSO 4 7H 2 O 0.01g, MgSO 4 7H 2 O 0.5g soluble starch 20g, pH7.2~7.4, distilled water 1000ml.
[0047] 4. Potassium bacteria medium
[0048] Yeast Extract 0.4g, Mannitol 10.0g, K 2 HPO 4 0.5g, MgSO 4 ...
Embodiment 2
[0066] Composition of compound bacterial agent
[0067] A rural domestic garbage and sludge decomposing bacteria agent is prepared as follows (by weight):
[0068] Table 1 The composition of rural domestic waste and sludge decomposing bacteria agent
[0069] name
[0070] The number of effective viable bacteria is 5×10 8 piece / g;
Embodiment 3
[0072] Mutual antagonism experiment between strains
[0073] The activated Bacillus subtilis, Streptomyces flavinus, Trichoderma longifolia, Aspergillus niger and Bacillus peptone-like were subjected to the mutual antagonism experiment between the strains, and they were combined in pairs, divided into 20 combinations in total, and each combination had three repeat.
[0074] The steel ring method was used for the antibacterial test, and the strain was cultured in the corresponding medium for 3-7 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibacter...
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