Wheat yellow dwarf disease nature resisting phenotype identification method

Abstract

A wheat yellow dwarf disease nature resisting phenotype identification method is capable of identifying the resistance of wheat materials on all epidemic strains of local yellow dwarf viruses at a time, high in pertinence, high in detection efficiency and good in accuracy. The method comprises the steps that wheat seedlings are planted outdoors, wheat seedlings are planted indoors, wheat leaves with attached schizaphis graminum are collected in an early sown wheat field and are gently put on the wheat seedlings planted in a nutritive bowl, and the schizaphis graminum can be atomically transferred to the fresh wheat seedlings; when the outdoor low temperature is stabilized be higher than 0 DEG C in the next year, the schizaphis graminum reproduced indoors is transferred to the wheat seedlings in an arch shed; when the outdoor low temperature is maintained to be higher than 10 DEG C, the wheat leaves in the arch shed are sheared into small sections of 1.5 cm to 2.0 cm, the schizaphis graminum on the wheat seedlings in the arch shed is shaken off and put on the sheared wheat leaf sections, stirring is performed gently to enable the schizaphis graminum on the wheat leaf sections to be distributed evenly, and each wheat leaf section has 5 to 8 pieces of the schizaphis graminum. The prepared wheat leaf sections with the schizaphis graminum are clamped up through forceps and are gently put on the basal parts of the stems of the wheat plants to be identified, inoculation is carried out in a single plant mode, and each plant is inoculated with 5 to 8 pieces of the schizaphis graminum.

Description

technical field [0001] The invention belongs to the technical field of wheat disease resistance identification, and specifically relates to screening of disease-resistant germplasm resources, identification of variety resistance and tolerance, and generation selection of wheat yellow dwarf resistance breeding. Background technique [0002] Wheat yellow dwarf disease is a viral disease caused by barley yellow dwarf virus (BYDV) mediated by aphids. It is mainly distributed in North China, Northwest, Northeast and other wheat areas. For "Wheat Cancer". Wheat infection causes physiological dysfunction, reduced effective tillers, yellowing of leaves, dwarfing of plants, reduction of seed setting rate and thousand-grain weight, and quality decline. In general years, the yield is reduced by 5-10%, and in popular years, the yield is reduced by 20-30%. In severe cases, it can reach 50% % above, posing a great threat to wheat production. Different germplasm or varieties have differe...

AI Technical Summary

Problems solved by technology

The first is to use the stack test method to divide the virus strains for field inoculation identification. The prevalence of wheat yellow dwarf strains in different ecological regions is different, and several strains often occur in the same ecological region. This method is used to identify anti-GAV The resistance of the wheat materials of the same strain to other strains (such as GPV) is unknown, and it is necessary to increase the workload if the resistance to all the strains prevailing in the area is to be identified; in addition, this method is limited to It is limited to stable wheat lines and cannot be applied to the generation separation and selection of disease resistance breeding research, which is the most critical link in cultivating yellow dwarf resistance wheat varieties

The second is the identification of molecular markers based on PCR. At present, there are many yellow dwarf resistance genes introduced into common wheat. The application of molecular markers can accurately identify whether wheat materials contain yellow dwarf resistance genes. However, due to the interaction between genes and gene Due to the difference in the expression of the disease itself, the expression of the disease resistance gene contained in it is sometimes suppressed or disturbed, so the material containing the yellow dwarf disease resistance gene identified by this method may show disease-susceptible characteristics in production and application