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Brevibacterium frigoritolerans as well as microbial inoculant and application of brevibacterium frigoritolerans and microbial inoculant

A cold-resistant Brevibacterium and microbial agent technology, applied in the field of agricultural biology, can solve problems such as difficult to establish to control nematode population density, unstable control effect, low germination rate, etc.

Active Publication Date: 2014-07-30
YANTAI DIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method of preventing and treating nematodes includes chemical drug method and biological control method, and chemical control method is such as carrying out soil fumigation with bromide, vibomu, bisulfide, lime nitrogen, etc., but this type of soil fumigant has biocidal properties and can Kill pests, weeds, rats and beneficial microorganisms in the soil; biological control methods, such as using nematode bio-control fungi to kill nematodes, nematode bio-control fungi usually have a low germination rate and it is difficult to establish the population density required to control nematode growth. As a result, they have the defects of low control efficiency and unstable control effect in nematode control

Method used

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  • Brevibacterium frigoritolerans as well as microbial inoculant and application of brevibacterium frigoritolerans and microbial inoculant
  • Brevibacterium frigoritolerans as well as microbial inoculant and application of brevibacterium frigoritolerans and microbial inoculant
  • Brevibacterium frigoritolerans as well as microbial inoculant and application of brevibacterium frigoritolerans and microbial inoculant

Examples

Experimental program
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preparation example Construction

[0017] Wherein, the preparation method of the microbial agent may include: inoculating the Brevibacterium cold-tolerant of CGMCC No.8837 in a fermentation medium for culturing. Preferably, in each gram of the microbial agent obtained by culturing, the number of live bacteria of Brevibacterium cold-resistant with a preservation number of CGMCC NO.8837 can be (1-500)×10 7 CFU, more preferably (5-150)×10 7 CFU. Wherein, the cultivation temperature may be 28-30°C, and the cultivation time may be 16-25 hours.

[0018] The present invention also provides the application of the above-mentioned Brevibacterium cold-tolerant and the above-mentioned microbial inoculum in preventing and controlling crop diseases.

[0019] Particularly preferably, the crop diseases include nematode diseases. More preferably, the nematodes include root-knot nematodes and / or cyst nematodes.

Embodiment 1

[0022] This example is used to illustrate the cultivation of Brevibacterium cold-tolerant and the preparation of microbial inoculum of the present invention.

[0023] Brevibacterium frigoritolerans with preservation number CGMCC NO.8837 was inoculated into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 30°C and Cultivate to bacterial density OD under shaking at 120 rpm 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0024] Add 100mL of the above-mentioned seed bacteria liquid into 100L of fermentation medium (fermentation medium contains 80g / L of soybean powder, 80g / L of starch, 3g / L of sodium chloride, 3g / L of calcium carbonate, 5g / L of phosphoric acid Potassium dihydrogen and ferrous sulfate of 5g / L), cultivated at 30°C, sampled during the cultivation process and observed by direct microscope counting until the number of viable bacteria of the cold-resistant Brevibacterium per gram of culture solution was 108C...

Embodiment 2

[0026] According to the method of Example 1, the microbial inoculant was prepared, the difference being that the fermentation medium used was to contain 60g / L of soybean powder, 100g / L of starch, 2g / L of sodium chloride, 4g / L of calcium carbonate, 3g / L potassium dihydrogen phosphate and 3g / L ferrous sulfate medium.

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Abstract

The invention provides brevibacterium frigoritolerans of which the collection number is CGMCC NO.8837. The invention also provides a microbial inoculant containing thalli and a culture medium, wherein the thalli comprises brevibacterium frigoritolerans of which the collection number is CGMCC NO.8837. The invention also provides applications of brevibacterium frigoritolerans and the microbial inoculant in preventing and treating crop diseases. By adopting the technical scheme, brevibacterium frigoritolerans and the microbial inoculant disclosed by the invention can be used for preventing and treating nematode diseases of crops more effectively.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a cold-resistant Brevibacterium, a microbial bacterial agent and applications thereof. Background technique [0002] The diseases caused by nematodes are one of the crop diseases that have become more and more serious in recent years. Generally, the affected crops can reduce the yield by 20-30%, and the severe yield reduction can reach 60%, or even extinction. The degree of damage is quite serious. There are more than 3,000 types of nematodes that can cause harm, and the main types of nematodes include southern root nematodes and Javan nematodes. Nematodes live through the winter as adults, eggs on diseased plant residues or as larvae in the soil, and can survive for 3 years without a host. In relatively benign conditions, the eggs develop into first instar larvae within a few hours. The second instar larvae are very active and invade from the young root tip of the hos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/13
Inventor 吕昭辉阮志勇赵秉强王慧敏赵君才赵文勇刘慧宋昭王彦伟刘小飞
Owner YANTAI DIYUAN BIOTECH
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