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A scaled quantitative real-time diagnosis microfluidic chip and its preparation method

A microfluidic chip and scale technology, applied in chemical instruments and methods, instruments, laboratory utensils, etc., can solve the problems of time-consuming, complex processing technology, and the use of dangerous chemical reagents, and achieve easy manufacturing, operation, and structure. simple effect

Active Publication Date: 2016-06-08
SUZHOU DIYINAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the chemical corrosion processing technology adopted by the V-chip, the processing technology is complex, time-consuming, and dangerous chemical reagents are used, which is not conducive to the chip processing and practical promotion and application; in addition, due to the linkage design of the V-chip, it can detect Multiple targets in the same sample, but unable to detect multiple different samples at the same time

Method used

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  • A scaled quantitative real-time diagnosis microfluidic chip and its preparation method
  • A scaled quantitative real-time diagnosis microfluidic chip and its preparation method
  • A scaled quantitative real-time diagnosis microfluidic chip and its preparation method

Examples

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preparation example Construction

[0041] The preparation method of the scale-type quantitative real-time diagnostic microfluidic chip includes the following steps:

[0042] 1) Use laser engraving or hot embossing to prepare a hollow area on the middle surface layer 11, then laser engrave the sampling hole 8 and the sampling hole 9 on the surface chip plate 10, and print the scale line 3 and The scale value corresponding to the scale line is 4; then the surface layer chip plate and the middle surface layer 11 are bonded to form a chip plate after aligning the corners;

[0043] 2) After step 1), the indicator liquid is dropped into the hollow area, and the dropping position corresponds to the position of the microchannel, and the dropped indicator liquid divides the hollow area into two sections;

[0044] 3) After step 2), the base layer 12 and the middle surface layer on the chip board are aligned through the corners and bonded tightly.

[0045] Before the base layer 12 is bonded to the chip board, the base la...

Embodiment

[0047] A scaled quantitative real-time diagnostic microfluidic chip for the measurement of catalase activity in plant tissues. The base plate (basal layer) is an ordinary microscope slide with a size of 76.20mm×25.40mm; the chip plate is made of a layer of plexiglass The board is composed of a layer of 3M transparent optical double-sided adhesive with a hollow area, and the size is the same as the base board. Scale lines, scale values, sample injection holes and sample output holes are set on the plexiglass plate; the hollow area on the 3M transparent optical double-sided adhesive includes the corresponding shapes of microchannels, buffer pools and microreaction chambers, and the base plate and the chip plate are glued together. After the combination, a microchannel, a buffer pool and a microreaction chamber connected in sequence are formed; the buffer pool is a rounded rectangle, the outlet (air outlet) of the microchannel (linear) communicates with the external environment, a...

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Abstract

The invention provides a scale type micro-fluidic chip for quantitative immediate diagnosis and a preparation method of the scale type micro-fluidic chip. The scale type micro-fluidic chip comprises micro-reaction chambers and micro-channels, wherein the micro-reaction chambers have sample feeding holes and sample discharging holes, openings at one ends of the micro-channels are communicated to external environments, and indicating liquid is locally encapsulated in the micro-channels; scale marks and corresponding numerical values are arranged outside the micro-channels to facilitate the quantitative reading of a detection result, the other ends of the micro-channels are communicated with the micro-reaction chambers through buffer tanks to form complete independent working units, and meanwhile, multiple working units can be arrayed on a chip to realize multichannel detection. The scale type micro-fluidic chip has the advantages of no complex instruments, convenience in carrying, low cost, simplicity and convenience in operation, easiness in reading, multi-target detection, little use amount of sample, sensitivity and the like and can be widely applied to biochemical analysis fields including medical diagnosis, food security, environmental pollution and the like.

Description

technical field [0001] The invention relates to the field of biomedical real-time diagnosis, in particular to a scaled quantitative real-time diagnosis microfluidic chip and a preparation method thereof. Background technique [0002] The microfluidic chip provides an efficient and fast new reaction platform, which uses advanced micro-nano processing technology to integrate the sample pretreatment, reaction, separation and detection required in traditional biochemical analysis methods into one chip (so Also known as lab-on-a-chip), it can automatically complete the entire analysis process, has the advantages of less sample consumption, time-saving, high-throughput, etc., and has been widely used in biochemical analysis, medical diagnosis and other fields. [0003] The traditional quantitative detection microfluidic chip is based on glass, silicon wafer, organic silicon polymer, etc., and is processed by chemical corrosion or soft lithography technology. The preparation proces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N35/00
Inventor 徐峰崔兴业胡杰卢天健
Owner SUZHOU DIYINAN BIOTECHNOLOGY CO LTD
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