Scale type micro-fluidic chip for quantitative immediate diagnosis and preparation method of scale type micro-fluidic chip
A microfluidic chip, scale technology, applied in chemical instruments and methods, instruments, laboratory utensils, etc., can solve the problems of complex processing technology, time-consuming, use of dangerous chemical reagents, etc., to achieve the realization of operation, easy manufacturing, structure simple effect
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[0041] The preparation method of the scale-type quantitative real-time diagnostic microfluidic chip includes the following steps:
[0042] 1) Use laser engraving or hot embossing to prepare a hollow area on the middle surface layer 11, then laser engrave the sampling hole 8 and the sampling hole 9 on the surface chip plate 10, and print the scale line 3 and The scale value corresponding to the scale line is 4; then the surface layer chip plate and the middle surface layer 11 are bonded to form a chip plate after aligning the corners;
[0043] 2) After step 1), the indicator liquid is dropped into the hollow area, and the dropping position corresponds to the position of the microchannel, and the dropped indicator liquid divides the hollow area into two sections;
[0044] 3) After step 2), the base layer 12 and the middle surface layer on the chip board are aligned through the corners and bonded tightly.
[0045] Before the base layer 12 is bonded to the chip board, the base la...
Embodiment
[0047] A scaled quantitative real-time diagnostic microfluidic chip for the measurement of catalase activity in plant tissues. The base plate (basal layer) is an ordinary microscope slide with a size of 76.20mm×25.40mm; the chip plate is made of a layer of plexiglass The board is composed of a layer of 3M transparent optical double-sided adhesive with a hollow area, and the size is the same as the base board. Scale lines, scale values, sample injection holes and sample output holes are set on the plexiglass plate; the hollow area on the 3M transparent optical double-sided adhesive includes the corresponding shapes of microchannels, buffer pools and microreaction chambers, and the base plate and the chip plate are glued together. After the combination, a microchannel, a buffer pool and a microreaction chamber connected in sequence are formed; the buffer pool is a rounded rectangle, the outlet (air outlet) of the microchannel (linear) communicates with the external environment, a...
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