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A method for preparing colloidal gold-labeled nanoliposomes loaded with PD drugs

A nanoliposome and colloidal gold technology, applied in the field of biological sciences, can solve the problems of difficulty in crossing the BBB, poor brain targeting, macrophage phagocytosis, etc., achieving small particle size, high encapsulation efficiency, and good stability. Effect

Active Publication Date: 2016-01-20
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the particle size of the currently prepared medicinal liposomes is generally larger than 100nm, which is easily phagocytized by macrophages, difficult to cross the BBB, and has poor brain targeting

Method used

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  • A method for preparing colloidal gold-labeled nanoliposomes loaded with PD drugs
  • A method for preparing colloidal gold-labeled nanoliposomes loaded with PD drugs
  • A method for preparing colloidal gold-labeled nanoliposomes loaded with PD drugs

Examples

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Embodiment 1

[0023] Example 1 A method for preparing colloidal gold-labeled L-dopa nanoliposomes (the encapsulation rate of L-dopa is 62.52%, and the encapsulation rate of colloidal gold is 72%)

[0024] according to m 大豆卵磷脂 :m 胆固醇 =8:1, weigh soybean lecithin and cholesterol, dissolve in absolute ethanol; according to m L-dopa :m 胶体金 :m 大豆卵磷脂 =20:1:2000, absorb the corresponding L-dopa mother solution and colloidal gold mother solution, add a certain amount of PBS (pH=7.4) solution to dissolve. Add the PBS solution of colloidal gold L-dopa into a 20mL beaker, place it in a water bath at 45~55°C, add lecithin and cholesterol absolute ethanol solution dropwise while stirring, and continue stirring at constant temperature until the ethanol evaporate completely. Sonicate the liposome suspension in a water bath for 10 minutes to obtain a pale pink liposome suspension, slowly inject the liposome suspension into a centrifuge tube containing a sucrose density gradient solution, centrifuge at...

Embodiment 2

[0034] Example 2 A method for preparing colloidal gold-labeled amantadine hydrochloride nanoliposomes (encapsulation rate of amantadine hydrochloride is 56.54%, colloidal gold encapsulation rate is 76.78%)

[0035] according to m 大豆卵磷脂 :m 胆固醇 =8:1, weigh soybean lecithin and cholesterol, dissolve in absolute ethanol; according to m 盐酸金刚烷胺 :m 胶体金 :m 大豆卵磷脂 =20:1:1200, absorb the corresponding amantadine hydrochloride mother solution and colloidal gold mother solution, add a certain amount of PBS (pH=7.4) solution to dissolve. Add the PBS solution of colloidal gold amantadine hydrochloride into a 20mL beaker, place it in a water bath at 45~55°C, add the absolute ethanol solution of lecithin and cholesterol dropwise while stirring, and continue stirring at constant temperature until Ethanol evaporates completely. Sonicate the liposome suspension in a water bath for 10 minutes to obtain a pale pink liposome suspension, slowly inject the liposome suspension into a centrifuge tu...

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Abstract

The invention discloses a method for preparing nano-liposome marked by colloidal gold and loaded with a PD (pharmacodynamics) medicine. The method comprises the following steps: dispersing lecithin and a cholesterol and ethanol solution in a PBS (Phosphate Buffer Solution) containing colloidal gold and levodopa or amantadine PD therapeutic medicine under specific conditions, then adding the PBS solution into a water bath to stir, completely volatilizing the ethanol and then performing appropriate ultrasonic treatment to prepare the nano-liposome. The prepared nano-liposome marked by colloidal gold and loaded with the PD medicine is small in particle diameter and excellent in stability; meanwhile, the nano-liposome is located and marked by the gold, thereby improving the stability of the PD medicine, improving the bioavailability, reducing the usage amount of the medicine and reducing the toxicity. The preparation method is simple to operate; the reaction is easy to control; the product, namely the nano-liposome can be used for researching the medicine administration effect of the nano-levodopa or amantadine liposome, improving the toxic and side effects of the levodopa or the amantadine and enhancing the brain targetability. The product also can be used as a probe which is used for researching a cerebral metabolism pathway of the nano-liposome.

Description

technical field [0001] The invention relates to a method for preparing nano-liposomes, in particular to a method for preparing colloidal gold-labeled nano-liposomes loaded with PD drugs (levodopa or amantadine), and belongs to the technical field of biological sciences. Background technique [0002] Parkinson's disease (Parkinson's disease, PD) is a common neurodegenerative disease, more common in the elderly, with an average age of onset of around 60 years old, and young Parkinson's disease with onset under the age of 40 is rare. The survey in 2001 showed that among people over 50 years old (regardless of gender), the prevalence rate has reached 1~2%. In my country, the prevalence of PD among people over 65 years old is about 1.7%. The main pathological change of Parkinson's disease is the degeneration and death of DA (dopamine, dopamine) neurons in the substantia nigra of the midbrain, which leads to a significant decrease in the content of DA in the striatum and causes t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K49/00A61K47/02A61K31/198A61K31/13A61P25/28
Inventor 耿丽娜常彦忠李亚永胡跃
Owner HEBEI NORMAL UNIV
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