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Heat shock transcription factor and application thereof

A technology of heat shock transcription factor and transcription factor, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as dwarfing and plant growth delay, and achieve the effect of improving heat-resistant traits and plant heat-resistant traits

Inactive Publication Date: 2014-07-16
BEIJING INST OF LANDSCAPE ARCHITECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overexpression of HsfA genes, such as AtHsfA2 and OsHsfA2e, can improve the tolerance of transgenic plants under stress. However, with the improvement of heat and salt tolerance, the plants show growth delay and dwarfing.

Method used

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  • Heat shock transcription factor and application thereof
  • Heat shock transcription factor and application thereof
  • Heat shock transcription factor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Experimental materials The seedlings differentiated from the small bulbs of Lilium longiflorum 'White Heaven' (Lilium longiflorum Thunb.var.scabrum Masamune, 'White heaven') were grown on MS medium for 30 days, and placed at 37°C for 1 hour , taking its leaves for RNA extraction.

[0037] gene cloning

[0038] Total RNA was extracted using Invitrogen Trizol reagent (Cat. No.: 15596-026), and the specific operations were as follows:

[0039] Specific steps

[0040] 1) Take 0.1g (grams) of fresh leaves and put them into a 1.5ml (milliliter) centrifuge tube, add a small amount of liquid nitrogen.

[0041] 2) When the liquid nitrogen is about to evaporate, quickly grind the material into powder with a pestle, add 1ml Trizol, and mix well.

[0042] 3) Stand at room temperature for 3 minutes (minutes), add 200 μl of chloroform, shake vigorously for 30 seconds (seconds), and stand at room temperature for 5 minutes.

[0043] 4) Centrifuge at 12,000 g for 15 min at 4°C, and...

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Abstract

The invention provides a heat shock transcription factor, a nucleotide sequence encoding the heat shock transcription factor and an application thereof in improving the heat resistance of the plant. Overexpression of the heat shock transcription factor can remarkably improve the heat resistance of a transgenic plant without bringing harmful effects such as growth delay and stuntedness to the plant, so that the heat shock transcription factor has a unique advantage in improving the heat resistance of the plant.

Description

technical field [0001] The invention relates to the field of biology, in particular to plant heat shock transcription factors and applications thereof. Background technique [0002] Through high temperature pretreatment, plants can acquire certain heat tolerance. As an important molecular chaperone, the accumulation of heat shock proteins (Hsps) plays a key role in this process. The expression of genes encoding heat shock proteins is regulated at the transcriptional level by heat shock transcription factors (Hsfs), which bind to specific heat shock elements (heat shock elements, HSE) in the promoter region of heat shock protein genes. , to further activate its expression (Vierling et al., 1991; Sun et al., 2002). [0003] The Hsf gene was first cloned from yeast, and then successively cloned in Drosophila and mammals (Wu1995; Morimoto1998; et al., 1998; Nover et al., 2001). Heat shock transcription factors mainly include the following important structural domains: DNA b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/10C12N15/84C12N1/21A01H5/00
CPCC07K14/415C12N15/8271
Inventor 辛海波张华张华丽连青龙钟雄辉王涛宋利娜赵正楠董爱香义鸣放丛日晨
Owner BEIJING INST OF LANDSCAPE ARCHITECTURE
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