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Preparation method of injectable natural polysaccharide self-healing hydrogel

A technology of natural polysaccharides and hydrogels, applied in medical preparations of non-active ingredients, pharmaceutical formulations, pharmaceutical sciences, etc., to achieve the effects of mild reaction conditions, good biocompatibility, and simple synthesis process

Active Publication Date: 2014-07-09
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Injectable hydrogels used in biomedicine need to meet several key requirements: (1) The injectable hydrogel itself and its precursor solution should have good biocompatibility; (2) The injectable hydrogel should have good biocompatibility; The precursor solution needs to have the ability of rapid prototyping and solidification at the target site; (3) After the precursor solution gels and solidifies at the target site, it needs to quickly reach a certain mechanical strength to prevent the surrounding tissue from being squeezed or deformed. However, it is difficult for traditional injectable hydrogels to meet the above three requirements at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] This embodiment includes the following steps:

[0015] Step 1: Dissolve 1.0g of chitosan with a deacetylation degree of 86% and a weight-average molecular weight of 100kDa in 50mL of deionized water to make a 2% chitosan solution, then add 1.46mL of acrylic acid to it, and stir at 50°C After reacting for 3 days, adjust the pH of the solution to 10-12 with 1mol / L sodium hydroxide solution, then dialyze the solution in deionized water with a dialysis bag with a molecular weight cut-off of 8000, change the water three times a day, freeze-dry after 5 days of dialysis, Obtaining the N-carboxyethyl chitosan whose degree of substitution is about 46%;

[0016] Step 2: Dissolve 1.0g of sodium alginate with a viscosity of 350mpa.s into 100mL of deionized water to make a 1% sodium alginate solution, then add 0.22g of sodium periodate to it, and stir the reaction at 25°C in the dark 5 hours. After the reaction was completed, 2 mL of ethylene glycol was added to terminate the reac...

Embodiment 2

[0020] This embodiment includes the following steps:

[0021] Step 1: Dissolve 1.0g of chitosan with a deacetylation degree of 86% and a weight-average molecular weight of 200kDa in 50mL deionized water to make a 2% chitosan solution, then add 1.10mL acrylic acid to it, and stir at 50°C Response 3 days. Finally, adjust the pH of the solution to 10-12 with 1mol / L sodium hydroxide solution, then dialyze the solution in deionized water with a dialysis bag with a molecular weight cut-off of 8000, change the water three times a day, freeze-dry after dialysis for 5 days, and obtain a degree of substitution of About 25% N-carboxyethyl chitosan;

[0022] Step 2: Dissolve 1.0g of sodium alginate with a viscosity of 350mpa.s into 100mL of deionized water to make a 1% sodium alginate solution, then add 0.54g of sodium periodate to it, and stir the reaction at 25°C in the dark 5 hours. After the reaction was completed, 2 mL of ethylene glycol was added to terminate the reaction for 1 h...

Embodiment 3

[0026] This embodiment includes the following steps:

[0027] Step 1: Dissolve 1.0g of chitosan with a deacetylation degree of 86% and a weight-average molecular weight of 500kDa in 50mL of deionized water to make a 2% chitosan solution, then add 1.83mL of acrylic acid to it, and stir at 50°C After reacting for 3 days, adjust the pH of the solution to 10-12 with 1mol / L sodium hydroxide solution, then dialyze the solution in deionized water with a dialysis bag with a molecular weight cut-off of 8000, change the water three times a day, freeze-dry after 5 days of dialysis, Obtaining the N-carboxyethyl chitosan whose degree of substitution is about 38%;

[0028]Step 2: Dissolve 1.0g of sodium alginate with a viscosity of 350mpa.s into 100mL of deionized water to make a 1% sodium alginate solution, then add 1.08g of sodium periodate to it, and stir the reaction at 25°C in the dark 5 hours. After the reaction was completed, 2 mL of ethylene glycol was added to terminate the react...

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Abstract

The invention relates to a preparation method of injectable natural polysaccharide self-healing hydrogel. The preparation method of the injectable natural polysaccharide self-healing hydrogel comprises the following steps: firstly synthesizing water soluble N-carboxyethyl chitosan by carrying out a Michael addition reaction on acrylic acid and chitosan, carrying out an oxidization reaction on sodium periodate and sodium alginate to obtain oxidized sodium alginate, then carrying out a dehydration condensation reaction on N-carboxyethyl chitosan and oxidized sodium alginate and on adipic dihydrazide and oxidized sodium alginate in a phosphate buffer liquid at the temperature of 37 DEG C, so as to respectively generate invertible imine bond and acylhydrazone bond, and finally preparing injectable natural polysaccharide macromolecular hydrogel with self-healing property. The preparation method of the injectable natural polysaccharide self-healing hydrogel has the advantages that a synthetic process of the injectable self-healing hydrogel is simple and green, reaction conditions are mild, the injectable self-healing hydrogel can form gel in a physiological environment and has self-healing capability, and the injectable self-healing hydrogel has a good application prospect in the fields of drug sustained release and the like.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method of an injectable natural polysaccharide self-healing hydrogel, specifically using N-carboxyethyl chitosan and adipate dihydrazide respectively combined with oxidized alginic acid Sodium generates imine bonds and acylhydrazone bonds to form injectable gels, and these reversible chemical bonds endow the hydrogels with good self-healing properties. Background technique [0002] Polymer hydrogel (hydrogel) is a kind of viscoelastic "soft and wet" material that swells a large amount of water in a three-dimensional polymer network. It has a wide range of applications in biomedical fields such as tissue engineering, biosensors, and slow-release carriers (used to embed and release drugs, therapeutic proteins, or cells). Injectable hydrogel is a type of smart hydrogel that can inject a precursor solution (an aqueous solution composed of hydrogel-forming com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L5/08C08L5/04C08K5/25C08B37/08C08B37/04A61L27/20A61L27/52A61L31/04A61L31/14A61K47/36
Inventor 陈咏梅魏钊杨建海
Owner XI AN JIAOTONG UNIV
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