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Extraction process for novel anti-diabetes traditional Chinese medicine allium macrostemon or effective fraction

An extraction process and compound technology, applied in the fields of biomedicine and traditional Chinese medicine research, can solve the problems of no obvious hypoglycemic effect, achieve novel chemical components and action targets, strong international competitiveness, and solve the effect of unclear action mechanism

Inactive Publication Date: 2014-07-09
NANJING DINGYE BIOPHARM IND GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pure Chinese medicinal preparations that still have no obvious hypoglycemic effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take fresh bulbs of garlic, steam thoroughly, dry, and simply pulverize to obtain dry bulbs of garlic; soak with 8 times the amount of 60% ethanol, heat and reflux for extraction twice to obtain a reflux extract; the reflux extract is 1 Concentrate under reduced pressure for an hour and 30 minutes to obtain a thick extract; knead and dissolve the thick extract obtained by concentration, suspend it in water, and adsorb with D101, AB-8, and HPD-100 adsorption resin columns respectively, and then use 60% The column was washed with ethanol, and each eluted fraction was evaporated to dryness under reduced pressure at 55°C to obtain the extract of each eluted fraction; each eluted extract was separated by HPLC separation method. The results show that: 1. D101, AB-8, HPD-100 adsorption resin columns have larger adsorption capacity; 2. In the desorption (elution) test, the desorption capacity of D101 resin is the highest, and the elution rates are respectively It is 96.8, 94.3 ...

Embodiment 2

[0021] Take fresh bulbs of garlic, steam thoroughly, dry, and simply pulverize to obtain dry bulbs of garlic; soak with 8 times the amount of 60% ethanol, heat and reflux for extraction twice to obtain a reflux extract; the reflux extract is 1 Concentrate under reduced pressure for an hour and 30 minutes to obtain a thick extract; knead and dissolve the thick extract obtained by concentration, suspend it in water, use D101 adsorption resin column for adsorption, and then use 5%, 10%, 30%, 60% respectively and 95% ethanol to wash the column, and each eluted fraction was evaporated to dryness under reduced pressure at 55° C. to obtain each eluted fraction extract; each eluted extract was separated by HPLC. The results show that: 1. The elution ability of 60% ethanol is the strongest, and the saponins can be basically eluted completely; 2. Therefore, 60% ethanol is the best elution, and the total saponin content reaches 45%.

Embodiment 3

[0023] Take fresh bulbs of garlic, steam thoroughly, dry, and simply pulverize to obtain dry bulbs of garlic; soak with 8 times the amount of 60% ethanol, heat and reflux for extraction twice to obtain a reflux extract; the reflux extract is 2 times each time. Concentrate under reduced pressure for 1 hour to obtain a thick extract; knead and dissolve the thick extract obtained by concentration, suspend it in water, adsorb it with a D101 macroporous adsorption resin column, and then wash the column with 60% ethanol. Evaporate to dryness under reduced pressure at 55°C to obtain extracts of each eluted fraction; each eluted extract is separated by means of HPLC separation. The results show that the total saponin content of the reflux extract can be increased by 5% to 55% by concentrating under reduced pressure for 2 hours each time than in 1.5 hours.

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Abstract

A purpose of the present invention is to provide a purification process for a traditional Chinese medicine allium macrostemon or an effective fraction, wherein the purification process has characteristics of stable quality and simple route, and is suitable for large-scale production. According to the present invention, the natural medicinal chemistry and molecular pharmacology combined method is adopted to study the diabetes treatment effect of the traditional Chinese medicine allium macrostemon and the effective substance basis in the field of cardiovascular protection, and determine the structure, the characteristic and the activity of the macrostemonoside compound and the like, and the new active components can be produced through compatibility of different proportions of monomers; and the extraction process has characteristics of simpleness, easily available raw materials, low cost and high extraction rate, and is suitable for large-scale industrial production, and advantages of easy drug quality control, low oral dose, high patient compliance and the like are provided.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine research. The invention relates to an extraction process of a new type of Chinese traditional Chinese medicine Xiebai compound or effective parts for diabetes, so as to study its curative effect in the aspect of anti-diabetic and cardiovascular protection. Mainly take the traditional Chinese medicine Xiebai as the research object, which belongs to the field of biomedicine. Background technique [0002] According to the World Health Organization, there will be 300 million people with diabetes in the world by 2025, of which 230 million will be in developing countries. Diabetes is mainly divided into type I and type II diabetes, of which more than 97% belong to type II diabetes. The current incidence of diabetes in China is about 5%, and the number of diabetic patients ranks second in the world after India. my country is in a period of high incidence of diabetes, especially in recent ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A61P3/10A61P9/00C07J71/00A61K125/00
Inventor 孙林
Owner NANJING DINGYE BIOPHARM IND GRP
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