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Preparation method of molecular imprinting electrochemical sensor for detecting serotonins

A technology of molecular imprinting and serotonin, which is applied in the field of analysis and detection, can solve the problems affecting the sensitivity of sensors, achieve the effect of improving detection sensitivity, sensitive and real-time detection, and increasing conductivity

Inactive Publication Date: 2014-06-25
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the molecularly imprinted layers electropolymerized onto the electrode are non-conductive or weakly conductive substances, which greatly affect the sensitivity of the sensor.

Method used

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  • Preparation method of molecular imprinting electrochemical sensor for detecting serotonins
  • Preparation method of molecular imprinting electrochemical sensor for detecting serotonins
  • Preparation method of molecular imprinting electrochemical sensor for detecting serotonins

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method for preparing a molecularly imprinted electrochemical sensor for detecting serotonin, comprising the following specific steps:

[0036] Step 1, electrode pretreatment: pass the glassy carbon electrode through 0.05μm A1 2 o 3 After the suspension is polished, rinse with double distilled water, then ultrasonically clean with absolute ethanol and deionized water for 1 min, and finally rinse with double distilled water and blow dry with nitrogen;

[0037] Step 2, preparation of rGO / PANI composite membrane modified electrode: prepare rGO sulfuric acid solution (0.3mol / L) with a concentration of 2μg / mL and ultrasonically treat it for 30min, then add aniline to prepare a concentration of 0.02mol / L and pass nitrogen gas to remove oxygen Sonicate for another 30 minutes. Immerse the glassy carbon electrode pretreated in step 1 into a sulfuric acid solution containing rGO and aniline, perform electropolymerization by cyclic voltammetry (-0.5-0.7V) and blow dry to obtain...

Embodiment 2

[0042] A method for preparing a molecularly imprinted electrochemical sensor for detecting serotonin, comprising the following specific steps:

[0043] Step 1, electrode pretreatment: pass the glassy carbon electrode through 0.05μm A1 2 o 3 After the suspension is polished, rinse with double distilled water, then ultrasonically clean with absolute ethanol and deionized water for 1 min, and finally rinse with double distilled water and blow dry with nitrogen;

[0044] Step 2, preparation of rGO / PANI composite membrane modified electrode: prepare rGO sulfuric acid solution (0.4mol / L) with a concentration of 6μg / mL and ultrasonically treat it for 50min, then add aniline to prepare a concentration of 0.04mol / L and pass nitrogen to remove oxygen Sonicate for another 50 minutes. The glassy carbon electrode pretreated in step 1 was immersed in a sulfuric acid solution containing rGO and aniline, electropolymerized by cyclic voltammetry (-0.3-0.9V) and dried to obtain a rGO / PANI com...

Embodiment 3

[0049] A method for preparing a molecularly imprinted electrochemical sensor for detecting serotonin, comprising the following specific steps:

[0050] Step 1, electrode pretreatment: pass the glassy carbon electrode through 0.05μm A1 2 o 3 After the suspension is polished, rinse with double distilled water, then ultrasonically clean with absolute ethanol and deionized water for 1 min, and finally rinse with double distilled water and blow dry with nitrogen;

[0051] Step 2, preparation of rGO / PANI composite membrane modified electrode: prepare rGO sulfuric acid solution (0.5mol / L) with a concentration of 10μg / mL and ultrasonically treat it for 60min, then add aniline to prepare a concentration of 0.08mol / L and pass nitrogen to remove oxygen Sonicate for another 60 minutes. The glassy carbon electrode pretreated in step 1 was immersed in a sulfuric acid solution containing rGO and aniline, electropolymerized by cyclic voltammetry (-0.2-0.8V) and dried to obtain a rGO / PANI co...

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Abstract

The invention discloses a preparation method of a molecular imprinting electrochemical sensor for detecting serotonins. The preparation method comprises the following steps: carrying out polishing and washing pre-treatment on a glassy carbon electrode; preparing a sulfuric acid solution containing rGO; adding aniline and mixing sufficiently to prepare an electric polymerization solution; immersing the glassy carbon electrode into the electric polymerization solution and carrying out electric polymerization reaction; reacting and drying to obtain an rGO / PANI composite film modified electrode; preparing a PBS (Phosphate Buffer Solution) containing functionalized gold nano grains, amino thiophenol and the serotonins and standing the solution to obtain a molecular imprinting self-assembling solution; inserting the rGO / PANI composite film modified electrode into the molecular imprinting self-assembling solution; carrying out the electric polymerization reaction by using a chronoamperometry; and immersing the electrode into the sulfuric acid solution to be treated to obtain the molecular imprinting electrochemical sensor for detecting the serotonins. According to the preparation method, the electrical conductivity of the surface of the electrode is improved by the sensor, the detection sensitivity and the selectivity to the serotonins are improved, and the sensitivity of the molecular imprinting sensor is enhanced.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a preparation method of a molecularly imprinted electrochemical sensor for detecting serotonin. Background technique [0002] Serotonin (Serotonin, 5-HT) is a monoamine neurotransmitter widely distributed in the central nervous system, which has important effects on human emotions, sleep and appetite. Therefore, rapid and accurate detection of serotonin Analytical assays aid in the diagnosis of clinical disease. Some analytical methods, such as fluorescence assay, enzyme-linked immunoassay, radioimmunoassay, chemiluminescence, and mass spectrometry, have been used for the analysis and determination of serotonin, but these methods are time-consuming, costly and require pretreatment. [0003] Electrochemical sensor is an important electronic device, because of its simple design, high sensitivity and low price, it is widely used in human body detection, c...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 周学敏姜慧君薛诚王溪韩青朱婉莹
Owner NANJING MEDICAL UNIV
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