Oenococcus oeni and application thereof
A technology of Oenococcus oeni and bacteria, applied in the field of bioengineering, can solve the problems of plant juice production, mixed fermentation without MLF bacteria and Zymomonas mobilis, etc.
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[0063] The technical solution of the present invention will be further described in detail through specific embodiments below.
[0064] The present invention is collected from more than 40 sources such as various kinds of fruit and vegetable juices, wineries and pickles in Zhejiang, through the combined treatment of enrichment culture and double antibiotic-anaerobic culture method, adopts deep static culture, then thin layer chromatography technology and gram Through combined screening with the staining and microscopy, more than 100 species of Oenococcus wine were obtained. The screening results of TLC to detect the malic acid-lactic acid fermentation capacity are as follows figure 1 As shown, figure 1 Middle lane 1, 8g / L lactic acid, lane 2, 8g / L DL-malic acid, lanes 3-9, re-screened Odonococcus, fermentation broth added 8g / L DL-malic acid, 25℃ Fermented for 4 days. From figure 1 It can be seen that the corresponding O. enococcus in lanes 3-9 has the ability to degrade malic ...
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