Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Oenococcus oeni and application thereof

A technology of Oenococcus oeni and bacteria, applied in the field of bioengineering, can solve the problems of plant juice production, mixed fermentation without MLF bacteria and Zymomonas mobilis, etc.

Inactive Publication Date: 2014-06-25
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current utilization of MLF-type bacteria is mainly to carry out malo-lactic fermentation after alcoholic fermentation of grape juice. Most of the other fermentations with Saccharomyces cerevisiae belong to exploratory reports, and there is no single use of MLF bacteria to produce pure plant yogurt drinks According to the report, there are no articles, patents and product reports on mixing MLF bacteria with Zymomonas mobilis to ferment plant juice to produce new fruit wine, or using MLF bacteria to debitter and enhance the flavor of non-alcoholic juice drinks.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oenococcus oeni and application thereof
  • Oenococcus oeni and application thereof
  • Oenococcus oeni and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] The technical solution of the present invention will be further described in detail through specific embodiments below.

[0064] The present invention is collected from more than 40 sources such as various kinds of fruit and vegetable juices, wineries and pickles in Zhejiang, through the combined treatment of enrichment culture and double antibiotic-anaerobic culture method, adopts deep static culture, then thin layer chromatography technology and gram Through combined screening with the staining and microscopy, more than 100 species of Oenococcus wine were obtained. The screening results of TLC to detect the malic acid-lactic acid fermentation capacity are as follows figure 1 As shown, figure 1 Middle lane 1, 8g / L lactic acid, lane 2, 8g / L DL-malic acid, lanes 3-9, re-screened Odonococcus, fermentation broth added 8g / L DL-malic acid, 25℃ Fermented for 4 days. From figure 1 It can be seen that the corresponding O. enococcus in lanes 3-9 has the ability to degrade malic ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an oenococcus oeni strain and an application thereof, and belongs to the technical field of bioengineering. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation name is (Oenococcus oeni)JQ88, the preservation date is 28th, June, 2013, the preservation number is CGMCC NO.7800, and the address is Beijing, China. The oenococcus oeni thallus is obtained by high-density fermenting, and then the oenococcus oeni thallus is uniformly mixed with a protective agent to obtain the thallus serum, the thallus serum is frozen and dried to obtain the freeze-dried powder; and the strain and the freeze-dried powder thereof can be applied to the secondary fermentation of fruit wine or grape wine, and can be applied to producing yogurt in the fermenting method of plant juice or animal milk. The oenococcus oeni strain malic acid-lactic acid is strong in fermentation capacity, the related beta-D-glycosidase is high in activity, the strain or the freeze-dried powder is applied to the secondary fermentation of the fruit wine and grape wine, and capable of endowing rich fruity and flowery for the fruit wine and grape wine product, and improving the taste thereof.

Description

Technical field [0001] The invention relates to a microbial strain and its application, in particular to an Odonococcus strain with strong malic acid-lactic acid fermentation capacity and its application in fruit wine brewing, belonging to the technical field of bioengineering. Background technique [0002] The brewing of wine and various fruit wines is divided into two parts: firstly, the first fermentation, which converts various sugars into alcohol by yeasts, etc.; secondly, the second fermentation, where a large amount of dibasic acid, especially malic acid, in the wine is converted by lactic acid bacteria It is transformed into mild monobasic acid such as lactic acid. This kind of fermentation is called malolactic fermentation (MLF). MLF reduces the acidity of wine, which not only has obvious effects, but also increases the flavor complexity and microbial stability of wine, giving the wine a delicate, soft and harmonious The taste, rich floral and fruity aroma, etc., have g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12G1/022C12G3/02A23C9/12C12R1/01
Inventor 袁辉
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products