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Method for identifying glossy privet fruit medicinal material or derivative

A kind of technology of Ligustrum lucidum and derivatives, applied in the identification field of natural medicinal plants

Active Publication Date: 2014-06-18
河南省科高植物天然产物开发工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the identification of privet medicinal materials or derivatives using IGD carbon NMR coupled fingerprint technology

Method used

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  • Method for identifying glossy privet fruit medicinal material or derivative
  • Method for identifying glossy privet fruit medicinal material or derivative
  • Method for identifying glossy privet fruit medicinal material or derivative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0225] Example 1: Ligustrum lucidum iridoid glycoside special extract IGD C-NMR coupled fingerprint

[0226] (1) Preparation of characteristic extracts

[0227] Weigh 50g of crushed Ligustrum lucidum medicinal material A (passed through a 24 mesh sieve), add 6 / 6 / 10 times the volume (300 / 300 / 500mL), 95% (volume ratio) of ethanol, and reflux extraction at 95°C 3 times, extract for 1 hour each time, filter and combine the filtrate, concentrate under reduced pressure to the extract; add 20 times the weight of the medicinal material in water (1000g) to the extract, disperse, and centrifuge; then use 1 times the weight of the medicinal material AB-8 Macroporous adsorption resin (50g) was packed into a column, washed with water, 60% ethanol and 90% ethanol successively, and the 60% ethanol eluate was collected, evaporated to dryness under reduced pressure, and the characteristic of iridoid glycoside A of Ligustrum lucidum medicinal material was obtained extract (CET).

[0228] (2) ...

Embodiment 2

[0240] Example 2: Ligustrum lucidum medicinal material B special extract IGD C-NMR coupled fingerprint

[0241] (1) Preparation of characteristic extracts

[0242] Weigh 50g of crushed Ligustrum lucidum medicinal material B (passed through a 50-mesh sieve), add 6 / 8 times the volume (300 / 400mL), 90% ethanol, and reflux at 90°C for 2 extractions, each extraction for 2 hours , after filtering, the filtrate was combined, concentrated under reduced pressure to extract; add 20 times the weight of medicinal materials in water (1000g) to disperse, and centrifuge; then pack HP-20 macroporous adsorption resin (75g) with 1.5 times the weight of medicinal materials , washed the column with water, 70% ethanol and 95% ethanol successively, collected the 70% ethanol eluate, and evaporated to dryness under reduced pressure to obtain the characteristic extract (CET) of Ligustrum lucidum medicinal material B iridoid glycosides.

[0243] (2) IGD carbon NMR fingerprint detection of the character...

Embodiment 3

[0254] Example 3: Ligustrum lucidum iridoid glycoside special extract IGD C-NMR coupled fingerprint of Ligustrum lucidum medicinal material C

[0255] Weigh Ligustrum lucidum medicinal material C (100g) and pulverize (through a 30-mesh sieve), ultrasonically extract twice, each extraction takes 1.5 hours, and the temperature of each extraction is 55°C; after filtering, combine the filtrates, and concentrate under reduced pressure to obtain an extract; Add water (2000g) 20 times the weight of the medicinal material to the extract to disperse, centrifuge; then use HP-20 macroporous adsorption resin (150g) with 1.5 weight of medicinal material to pack, wash with water, 50% ethanol and 90% ethanol successively column, collect 50% ethanol eluate, and evaporate to dryness under reduced pressure to obtain the characteristic extract of privet lucidum iridoid glycosides (CET).

[0256] (2) IGD carbon NMR fingerprint detection of the characteristic extract of C iridoid glycosides from L...

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Abstract

The invention relates to a method for identifying a glossy privet fruit medicinal material or a derivative. The method comprises the following steps: extracting the glossy privet fruit medicinal material or the derivative to obtain a glossy privet fruit iridoid glycoside characteristic extract containing an active ingredient group; performing IGD (Inverted Gated Decoupling) nuclear magnetic resonance carbon spectrum fingerprint detection on the glossy privet fruit iridoid glycoside characteristic extract, and obtaining characteristic peak intensities of a plurality of active ingredients in the glossy privet fruit iridoid glycoside characteristic extract according to the fingerprint; measuring the characteristic peak intensity of a standard reference object corresponding to each active ingredient in the same manner; measuring the absolute content of the standard reference object through a quantitative analysis method; calculating the content of each active ingredient and the content of the active ingredient group in the glossy privet fruit medicinal material or a derivative by using the ratio of the characteristic peak intensities and the absolute content of the standard reference object. According to the method, the glossy privet fruit iridoid glycoside compounds contained in the glossy privet fruit and the ratio of the compounds can be reflected so as to achieve the aims of variety identification and quality determination of the glossy privet fruit medicinal material.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying medicinal materials or derivatives of Ligustrum lucidum. Background technique [0002] Ligustrum lucidum Ait. is the dry and mature fruit of Ligustrum lucidum Ait., a famous Chinese herbal medicine in my country. Ligustrum lucidum returns to the liver and kidney meridian, cool in nature, sweet and bitter in taste, has the effect of nourishing liver and kidney, improving eyesight and black hair [National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China 2010 Edition Part One. China Medical Science and Technology Press, 43] . Its active ingredients are mainly iridoid glycosides, which have the functions of lowering blood sugar, resisting hemorrhagic shock, inhibiting platelet aggregation, regulating immunity, antibacterial and anti-inflammatory [Liu Hong, et al. Journal of Nanjing University of Tr...

Claims

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Application Information

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IPC IPC(8): G01N24/08G01N30/02
Inventor 张海艳赵天增王伟董建军范毅常霞李飞飞郭唯陈飞魏悦陈玲李自红
Owner 河南省科高植物天然产物开发工程技术有限公司
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