Method for measuring lignin content of herbaceous plants
A herbal plant and a measuring method technology, which is applied in the field of measuring the lignin content of herbaceous plants, can solve the problems of large error of lignin content and the like, and achieve the effects of simple method, easy operation and correct analysis result.
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[0032] The method for measuring the lignin content of herbaceous plants comprises the following steps:
[0033] (1) Dry the collected plant samples at 80°C for 48-72 hours, use a miniature plant grinder to crush the plant samples, pass through a 30-mesh sieve after crushing, and collect the plant sample powder.
[0034] (2) Weigh 0.05~0.10g of plant sample powder, and record the specific value as n; then put the plant sample powder into a 50mL centrifuge tube, add 10mL of acetic acid solution with a mass concentration of 0.1%, stir it evenly with a glass rod, and place it for 20 After ~30min, fully softened plant sample powder is obtained.
[0035] (3) Cover the centrifuge tube with the fully softened plant sample powder inside, put it into the centrifuge, and centrifuge at 3500rpm for 5min to obtain the supernatant A and sediment A respectively; pour off the supernatant A, and use the mass concentration Wash precipitate A with 5 mL of 0.1% acetic acid solution.
[0036] (4)...
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