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Denitrifying bacterium capable of efficiently denitriding under condition of low carbon-nitrogen ratio and application thereof

A technology of denitrification and nitrification bacteria, applied in the direction of bacteria, chemical instruments and methods, water pollutants, etc., to achieve the effect of wide application, high efficiency and stable treatment effect

Active Publication Date: 2014-06-18
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the nitrogen and other nutrients contained in the secondary effluent of urban sewage, it is a key factor leading to the risk of eutrophication and algal blooms in the water body

Method used

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  • Denitrifying bacterium capable of efficiently denitriding under condition of low carbon-nitrogen ratio and application thereof
  • Denitrifying bacterium capable of efficiently denitriding under condition of low carbon-nitrogen ratio and application thereof
  • Denitrifying bacterium capable of efficiently denitriding under condition of low carbon-nitrogen ratio and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Isolation and identification of Bacillus sp. DN-LWX4

[0043] 1. Isolation of Bacillus sp. DN-LWX4

[0044] Put 10 filter materials at the outlet of the denitrification filter (see Table 1 for the water quality on the day of sampling) into 40ml of enrichment medium (1 liter of liquid prepared with 5g of beef extract, 10g of peptone, 1g of potassium nitrate and water, pH Cultivate overnight at 30°C in a 100ml Erlenmeyer flask (7.0-7.2), let the microorganisms on the filter material disperse, enrich and grow, draw 1ml of the bacterial suspension into a centrifuge tube filled with 9ml of sterile water, and obtain a dilution of 10 -1 According to this method, the bacterial suspension was diluted with sterile water to 10 -15 , to obtain a series of dilution gradient bacterial suspension. Draw 0.1ml dilution 10 respectively -8 --10 -15 Bacteria suspension to denitrifying bacteria isolation medium (1 liter of liquid prepared with 5g of beef extract, 10g of pepto...

Embodiment 2

[0067] Embodiment 2, the preparation of denitrifying bacteria agent

[0068]Put the slant medium (each liter of slant medium is prepared with 5g of beef extract, 10g of peptone, 1g of potassium nitrate, 18g of agar and water to make 1 liter of liquid; pH is 7.0-7.2; sterilized at 115°C for 20 minutes) The preserved Bacillus sp. (Bacillus sp.) DN-LWX4CGMCC No.8290 was picked with a sterilized inoculation loop and inoculated into 40ml of enrichment medium (5g of beef extract, 10g of peptone, Potassium nitrate 1g, potassium dihydrogen phosphate 0.4g and water are prepared into 1 liter of liquid, the pH is 7.0-7.2; sterilized at 115°C for 20 minutes) in a 100ml Erlenmeyer flask, shaken at 30°C for 12 hours to obtain The denitrifying bacteria solution is the denitrifying bacteria agent, and the content of Bacillus sp. DN-LWX4CGMCC No.8290 in the denitrifying bacteria agent is 1×10 15 cfu / ml.

Embodiment 3

[0069] Example 3. Effect of carbon source type on denitrification of Bacillus sp. DN-LWX4CGMCC No.8290

[0070] Prepare NO 3 - -N concentration of 110.13mg / L, and COD concentration of 1.87±0.10g / L four kinds of carbon source denitrification medium (carbon nitrogen ratio is 17:1), respectively, glucose denitrification medium, acetic acid Sodium denitrification medium, acetic acid denitrification medium and ethanol denitrification medium. Each liter of the four kinds of carbon source denitrification medium is made of carbon source, KH 2 PO 4 0.5g, MgSO 4 .7H 2 O0.2g, KNO 3 0.8g, trace elements 1mL (the solvent is high-purity water, the solute and its concentration are as follows: H 3 BO 3 2.86g / L; ZnSO 4 .7H 2 O0.22g / L; CuSO 4 .5H 2 O0.08g / L; MnSO 4 .4H 2 O2.03g / L; Na 2 MoO 4 .2H 2 O1.26g / L; pH7.0) and water to prepare 1 liter of liquid; pH is 7.0-7.2. The carbon source in the glucose denitrification medium is glucose, the carbon source in the sodium acetate de...

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Abstract

The invention discloses a denitrifying bacterium capable of efficiently denitriding under the condition of low carbon-nitrogen ratio and an application thereof. The denitrifying bacterium disclosed by the invention is Bacillus sp. DN-LWX4 which can be used to remove nitrate nitrogen, the perseveration number of the Bacillus sp. DN-LWX4 in China General Microbiological Culture Collection Center is No. 8290. The Bacillus sp. DN-LWX4 disclosed by the invention can be completely denitrified by glucose, ethanol, acetic acid, sodium acetate and other carbon sources, the removal rate of NO3-N can reach 95% when the initial concentration of NO3-N is 110.13mg / L; the carbon-nitrogen ratio of 3:1 is sufficient to satisfy the conditions of the bacterial demand for a carbon source, and the nitrogen removal rate of nitrate is 99.52% when the initial NO3-N concentration is 110.13mg / L.

Description

technical field [0001] The invention relates to a denitrifying bacterium which can efficiently denitrify under the condition of low carbon-to-nitrogen ratio and its application. Background technique [0002] Urban sewage recycling is an effective way to alleviate the shortage of water resources, prevent and control water environmental pollution, and improve the quality of water environment. The regenerated secondary effluent of urban sewage plants can be used for urban miscellaneous purposes, water source replenishment, landscape entertainment, industrial water, and water for agriculture, forestry, animal husbandry and fishery. However, nitrogen and other nutrients contained in the secondary effluent of urban sewage are the key factors leading to the risk of water eutrophication and algal blooms. Therefore, controlling the nitrogen concentration in reclaimed water is crucial for urban wastewater reuse to recharge surface water bodies such as rivers and lakes. [0003] Biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16
Inventor 孙迎雪廉巍雪周晓黎田媛
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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