Method for luring Chrysopa sinica Tjeder to restrict Toxoptera aurantii Boyer by pheromones
A Chinese lacewing and pheromone technology, applied in the direction of chemicals for biological control, botanical equipment and methods, applications, etc., to reduce pollution, reduce usage, and protect biodiversity
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Embodiment 1
[0016] 1. Using the headspace dynamic adsorption method to absorb the volatiles of tea shoots caused by tea aphids, rinse them with organic solvents, and identify more than 20 components by gas-mass spectrometry with standard samples, and also found α-farnesene , benzaldehyde and (trans)-2-pentenal are abundant.
[0017] 2. These more than 20 kinds of compounds and aphid sex pheromone components nepetalactone and nepetalin were respectively formulated into 10 -6 g / ml~10 -1 g / ml of the taste source, the antennae electrophysiology test was carried out with the Chinese lacewing as the test insect, and it was found that nepetalinol, nepetalactone, α-farnesene, benzaldehyde and (E)-2-pentenal caused Chinese lacewing EAG responded strongly.
[0018] 3. Mix nepetalactone and nepetalactone into a series of compounds in a certain ratio, and mix benzaldehyde, α-farnesene and nepetalin into a flavor source in a ratio of 1-2:2-3:5-7, Respectively (cis)-3-hexen-1-ol, (trans)-2-pentenal,...
Embodiment 2
[0021] The first step is the separation and identification of volatiles from tea shoots caused by tea aphids.
[0022] 1. Materials and methods:
[0023] The tea garden for testing is located in Meijiawu Village, Hangzhou City, Zhejiang Province. The tea tree variety is Longjing Changye Camellia sinensis cv. Longjingchangye, which is 20 years old.
[0024] Aphid damage to tea shoots: Before the experiment, the artificially raised wingless tea aphids were starved for 1 hour, and then 1000 tea aphids were placed on 6 healthy tea shoots of a tea tree in Longjing Changye in the experimental tea garden, and the tea leaves were covered with gauze. Cover it (to prevent the tea aphid from escaping), and after 24 hours, use a small brush to gently brush off the tea aphid and its honeydew on the tea tip, cut out these 6 tea tips with one bud and six leaf aphid, and weigh them, with a total mass of 15.5 g, Moisturize the base of the tea tip with absorbent cotton soaked in distilled wate...
Embodiment 3
[0051] Example 3 Experiment of trapping Central China lacewings in autumn and restricting tea aphids in spring of the following year in southern Anhui tea area
[0052] 1. Materials and methods
[0053] On October 31, 2012, Jingting Mountain Tea Plantation, Anhui Province, 0.5hm 2 In the pollution-free tea garden, place lures of Chinese lacewing traps at a distance of 8m×8m, and hang the lures on the tea shoots. Choose another piece of 0.5hm 2 The pollution-free tea garden is used as CK, and no lures are placed. In each tea garden, 10 sample plots were selected by parallel jumping method, and each sample plot was a 10-meter tea row, and the number of aphid tea shoots was recorded. On March 10, 2013, the same method was used to investigate the number of tea shoots with tea aphids.
[0054] 2. Results and analysis
[0055] At the end of October, the number of Chinese lacewings is large, and they can be lured around the aphids in the tea garden to prey on the tea aphids dire...
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