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Biological bactericide and preparation method thereof

A technology of biological fungicides and strains is applied in the field of microorganisms to achieve the effects of reducing foam, simple process flow and increasing yield

Pending Publication Date: 2014-06-04
河北三农农用化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report on N-o-chlorophenylacetyl-N-phenazine-1-carboxyl hydrazine as biological fungicide and preparation method thereof

Method used

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  • Biological bactericide and preparation method thereof
  • Biological bactericide and preparation method thereof
  • Biological bactericide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Use 10 1000mL Erlenmeyer flasks, each filled with 300mL medium A (2.0% glucose, 3.0% peptone, 0.5% beef extract, 0.35% magnesium sulfate and 0.3% potassium dihydrogen phosphate), sterilize at 120°C for 30 minutes, after cooling The activated Pseudomonas chloropinus strain SN36 bacteria solution was inoculated, placed at a temperature of 30° C., and shaken for 18 hours. Inoculate the cultured strain solution into 50L sterilized medium B, 2% viscous agent (dextrin, or xanthan gum, or sodium alginate, or carboxymethyl cellulose) according to the inoculation amount of 5%-10%. Sodium (CMC)) in a 100L fermenter, (medium B: starch 2.0%, lactose 1.0%, sucrose 1.0%, glycerin 0.5%, glucose 1.0%, peptone 1.0%, peanut powder 5.0%, soybean powder hydrolyzate 2.0% %, biological nitrogen 3.0%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.5% and iron sulfate 0.5%). At a temperature of 28°C 2-30°C, after 8-15 hours of aeration and stirring fermentation, the medium C (medium C...

Embodiment 2

[0031] Use 12 1000mL Erlenmeyer flasks, each containing 300mL medium A (glucose 2.0%, peptone 3.0%, beef extract 0.5%, magnesium sulfate 0.35%, and potassium dihydrogen phosphate 0.3%), sterilize at 120°C for 30 minutes, cool Afterwards, inoculate the activated Pseudomonas chloropinus strain SN36 bacterium solution, place it at a temperature of 30° C., and culture it in a shaker flask for 18 hours. Inoculate the cultured strain solution into 50L sterilized medium B, 5% viscous agent (agar, or soluble starch, or sodium alginate, or sodium carboxymethylcellulose ( CMC)) in a 100L fermenter, (medium B: starch 3.0%, lactose 2.0%, glycerin 0.6%, waste molasses 3.0%, glucose 1.0%, peptone 0.1%, peanut powder hydrolyzate 5.0%, soybean powder hydrolyzate 2.0%, biological nitrogen 2.0%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.5% and iron sulfate 0.5%), at a temperature of 28°C-30°C, after aeration and stirring for 10-15 hours, with 0.5-2.0 The speed of L / h is continuous...

Embodiment 3

[0036] Use 15 1000mL Erlenmeyer flasks, each filled with 300mL medium A (3.0% glucose, 3.0% peptone, 0.8% beef extract, 0.35% magnesium sulfate, 0.3% potassium dihydrogen phosphate), sterilize at 120 for 30 minutes, and inoculate after cooling The activated bacterial liquid of Pseudomonas chloropinus strain SN36 was placed at a temperature of 30° C. and shaken for 18 hours. Inoculate the cultured strain solution into a 100L fermenter with 50L sterile medium B and 8% viscous agent (soluble starch, or dextrin, or sodium alginate) at an inoculum size of 5%-20%, ( Medium B: starch 2.0%, lactose 2.5%, glycerin 1.5%, waste molasses 3.0%, glucose 1.0%, peptone 0.5%, peanut powder 5.0%, soybean powder 2.0%, biological nitrogen 4.0%, potassium dihydrogen phosphate 0.1 %, sodium chloride 0.5% and iron sulfate 0.5%), at a temperature of 28°C-30°C, after 10-15 hours of aeration and stirring fermentation, medium C (medium C: sucrose, molasses 2.0% , Glycerin 1.0%, Glucose 2.0%, Lactose 1....

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Abstract

The invention relates to a biological bactericide and a preparation method thereof. The biological bactericide is N-o-chlorophenylacetyl-N-phenazine-1-formylhydrazine which is obtained by virtue of two-step fermentation culture of a genetically improved strain SN36 of pseudomonas choloeaphtis in a liquid culture medium; the collection number of the obtained SN36 in the China General Microbiological Culture Collection Center is CGMCC No.7846. Compared with the prior art, the biological fermentation product has remarkable effects on prevention and treatment of fungal diseases of a plurality of crops, and mass production of the biological bactericide can be realized by virtue of introduction.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a biological bactericide and a preparation method thereof, in particular to a biological bactericide (N-o-chlorophenylacetyl-N-phenazine-1-carboxylhydrazide) and a preparation method thereof. Background technique [0002] Biological fungicide (N-o-chlorophenylacetyl-N-phenazine-1-carboxhydrazide) is mainly derived from the secondary metabolites of Pseudomonas chloropinus strains, and has the advantages of broad-spectrum disease resistance and resistance to drug resistance , is considered to be a natural antibacterial substance with high efficiency in inhibiting plant pathogenic bacteria. Has strong antifungal properties and also inhibits the activity of some bacteria. However, there is no report on N-o-chlorophenylacetyl-N-phenazine-1-carboxylhydrazine as a biological fungicide and its preparation method. Contents of the invention [0003] The purpose of the present inve...

Claims

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Application Information

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IPC IPC(8): C12P17/12C07D241/46A01P3/00A01P1/00C12R1/38
Inventor 刘书延张红艳
Owner 河北三农农用化工有限公司
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