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Method for detecting unknown poison by establishing liquid chromatography-mass spectrometry database

A liquid chromatography and database technology, applied in the field of detection of unknown poisons, can solve problems such as large differences in mass spectra, difficulty in unifying mass spectral libraries, and no mass spectra, and achieve the effects of improving detection efficiency, simple operation, and shortening detection and processing time.

Active Publication Date: 2014-05-28
BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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Problems solved by technology

However, since LC-MS generally adopts the method of soft ionization, and the mass spectrometry design principles of liquid chromatography-mass spectrometers of different companies are different, the analysis parameters are not the same, and there is no uniform comparable or matching mass spectrum. The differences can be large, which makes it difficult to unify mass spectral libraries using LC / MS

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  • Method for detecting unknown poison by establishing liquid chromatography-mass spectrometry database
  • Method for detecting unknown poison by establishing liquid chromatography-mass spectrometry database
  • Method for detecting unknown poison by establishing liquid chromatography-mass spectrometry database

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Embodiment 12010

[0053] Improper setting of identification method parameters and library retrieval parameters will lead to inaccurate search results. Therefore, when performing data screening, the relevant parameters of the two methods must be optimized first. The optimization of the identification method: the retention time deviation window is determined according to the retention time deviation of the substance in the actual sample and the retention time in the database; the mass number precision is a characteristic of high-resolution mass spectrometry, and the mass number deviation window is an important parameter to ensure mass accuracy. Generally, the difference between the two mass numbers is less than or equal to 3mDa. Optimization of the spectral library search method: Set appropriate matching factors, including the upper limit and lower limit settings, which are set according to the interference of the matrix in the actual sample. When the interference is large, the two parameters are r...

Embodiment 22008

[0056] Example 2 Screening of a poisoned sample in Wenshang, Shandong in February 2008

[0057] On February 4, 2008, after a family gathering of a family of villagers in Huzhuang Village, Kangyi Town, Wenshang County, Shandong Province, 18 people developed dizziness, pain, numbness and movement disorders (significantly in both lower limbs), accompanied by nausea and vomiting. No abdominal pain, diarrhea, fever and other symptoms, varying in severity. Five food samples (fried chicken, fried fish, etc.) used for gatherings were tested. About 2g of food samples were weighed into a 50mL centrifuge tube, 10mL of methanol was added to vortex and mixed, and ultrasonic extraction was performed for 10 minutes. After centrifugation, the supernatant was transferred to a 2ml sample bottle to collect its liquid chromatography mass spectrometry information, which was searched and compared with the established liquid chromatography-mass spectrometry database of common poisons and drugs. Salina ...

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Abstract

The invention relates to a method for detecting unknown poison by establishing a liquid chromatography-mass spectrometry database, in particular to a method used for detecting unknown poison during food poisoning. According to the method, firstly, an ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry method is used for establishing a liquid chromatography-mass spectrometry database of common poison; then, a sample is subjected to supersonic extraction with methyl alcohol or acetonitrile, liquid chromatography-mass spectrometry data of an extracting solution are measured similarly and searched and compared in the liquid chromatogram-mass spectrometry database of common poison according to the retention time of the sample and mass spectrometry fragments, and the variety of the unknown poison in the sample is judged; and the unknown poisoning sample is simply extracted and directly measured and compared, and a screening result can be acquired in one hour, so that the detecting and treating time of the sample is greatly shortened, the detecting efficiency is improved, and technical support is provided for related events such as food poisoning and the like caused by unknown reasons.

Description

Technical field [0001] The invention relates to a method for detecting unknown poisons, in particular to a method for constructing a liquid chromatography-mass spectrometry database to detect unknown poisons, especially for the detection of unknown poisons in food poisoning. Background technique [0002] Toxicant refers to a substance that acts on the body in a small dose under certain conditions, no matter how it is used, to produce physical or chemical effects with the body, causing the destruction of the body's normal physiological functions, causing a series of pathological changes, and even life-threatening and death. . Food poisoning refers to non-infectious acute and subacute diseases that occur after ingesting food containing biological or chemical toxic and harmful substances or taking toxic and harmful substances as food. According to statistics, there are hundreds to thousands of food poisoning incidents in my country each year, involving tens of thousands of people. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 邵兵张晶郭巧珍杨奕尹杰
Owner BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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