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Method for preparing ToRCH-ELISA (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent

A technology of diagnostic reagents and adsorbents, applied in the field of preparation of ToRCH-ELISA diagnostic reagents, can solve the problems of rheumatoid factor interference, long diagnosis time, poor repeatability, etc., and achieve good repeatability and strong specificity

Inactive Publication Date: 2014-05-21
严银芳
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Problems solved by technology

[0007] The purpose of the embodiment of the present invention is to provide a kind of preparation method of ToRCH-ELISA diagnostic reagent, aims at solving existing ToRCH-ELISA diagnostic kit and existing diagnosis time is long, sensitivity is poor, stability is poor, repeatability is poor and rheumatoid factor (RP ) to the problem of interference with the experiment

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  • Method for preparing ToRCH-ELISA (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent

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Embodiment Construction

[0020] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0022] Such as figure 1 Shown, the preparation method of the ToRCH-ELISA diagnostic reagent of the embodiment of the present invention comprises the following steps:

[0023] S101: Preparing pathogen antigens, Toxoplasma antigens are proliferated by intraperitoneal passage in mice. Rubella virus was propagated in Vero cells; herpes simplex virus types I and II were propagated in HeLa cells; cytomegalovirus was propagated in human embryonic lung diploid cells; t...

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Abstract

The invention discloses a method for preparing a ToRCH-ELISA (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent. The method for preparing the ToRCH-ELISA diagnosis reagent comprises the following steps: preparing a rubella virus, herpes simplex viruses I and II, a cytomegalovirus and toxoplasm antigen, and establishing an ELISA. The (Enzyme Linked Immunosorbent Assay Kit) diagnosis reagent is prepared by preparing the rubella virus, the herpes simplex viruses I and II, the cytomegalovirus and the toxoplasm antigen and establishing the ELISA. The method has the technical effects of rapidness, sensitivity, high specificity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of preparation of ToRCH-ELISA diagnostic reagents, in particular to a preparation method of ToRCH-ELISA diagnostic reagents. Background technique [0002] TORCH is a combination of four widely spread English prefixes of pathogens. To represents toxoplasma; R represents rubella virus; c represents cytomegalovirus; H represents herpes simplex virus type I and type II. Pregnant women are prone to primary infection due to changes in endocrine and immune status. And can be transmitted to the fetus or newborn through the placenta, birth canal or breast milk, causing miscarriage, stillbirth and deformity. Infected newborns can develop multi-system and multi-organ damage a few years later, with very serious consequences. Therefore, screening pregnant women is of great significance. We used the EILSA indirect method to qualitatively detect the specific anti-TORCH-IgM antibody in human serum. During the developme...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
CPCG01N33/569G01N33/531
Inventor 严银芳陈晓刘军
Owner 严银芳
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