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Preparation method and application of tobacco polysaccharide sulfate

A technology of polysaccharide sulfate and tobacco, which is applied in the direction of chemical instruments and methods, and compositions for inhibiting chemical changes, etc., which can solve the problems of unstable functions and poor structural stability, etc.

Active Publication Date: 2014-05-21
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although Tianre polysaccharide has many excellent properties, but due to its poor structural stability and unstable function, chemical modification methods are often used to further improve the polysaccharide function

Method used

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  • Preparation method and application of tobacco polysaccharide sulfate
  • Preparation method and application of tobacco polysaccharide sulfate
  • Preparation method and application of tobacco polysaccharide sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In order to check the feasibility of the antioxidant prepared by utilizing tobacco polysaccharide provided by the present invention, the inventor has done further experimental verification, and relevant experimental conditions are explained as follows:

[0034] Test material : First-cured tobacco leaves, grade C2F, the variety of tobacco leaves is Yuyan No. 10, and the place of origin is Xiangxian County, Henan Province.

[0035] Some experimental reagents and instruments: The manufacturer of phenanthroline is Tianjin Kemiou Chemical Reagent Co., Ltd., the manufacturer of absolute ethanol is Tianjin Fuyu Fine Chemical Co., Ltd., and the manufacturer of 30% (mass fraction) hydrogen peroxide is Yantai Shuangshuang Chemical Co., Ltd. , DMF is N,N-dimethylformamide, DPPH is 1,1-diphenyl-2-trinitrophenylhydrazine, other reagents are chlorosulfonic acid, pyridine, trichloroacetic acid, potassium sulfate, gelatin, FeSO 4 All reagents were purchased from the market, and it ...

Embodiment 2

[0079] In order to further detect and verify the tobacco polysaccharide sulfate prepared by the present invention, the inventors have done further detection and verification experiments. The inventor carried out infrared spectroscopic analysis on the tobacco polysaccharide and tobacco polysaccharide sulfate prepared in Example 1. The specific experimental process is as follows:

[0080] Take respectively 1 mg of tobacco polysaccharide and tobacco polysaccharide sulfate prepared in Example 1, press into tablets with 1 mg of potassium bromide (KBr), and scan the IR absorption with a Fourier transform infrared spectrometer in the interval of 4000 to 400 cm. Experimental results such as figure 1 shown.

[0081] From figure 1 Visible, 3299.0 cm -1 is the O-H stretching vibration, 2930.8 cm -1 is-CH 3 The stretching vibration of the base, which is the characteristic absorption peak of polysaccharides; 1603.0 cm -1 is N-H bending vibration; 1413.6 cm -1 is the variable-an...

Embodiment 3

[0083] The inventor utilizes the DPPH method to further detect and measure the antioxidant activity of the tobacco polysaccharide prepared in Example 1 and the tobacco polysaccharide sulfate ester, and the specific inspection process is as follows:

[0084] 1. Preparation of tobacco polysaccharide and tobacco polysaccharide sulfate test solution

[0085] Accurately weigh 0.010 g each of the tobacco polysaccharide and tobacco polysaccharide sulfate prepared in Example 1, respectively dissolve them in 10 ml of distilled water and mix thoroughly to prepare a 1 mg / mL mother liquor of tobacco polysaccharide and tobacco polysaccharide sulfate.

[0086] 2. Dilute the tobacco polysaccharide and tobacco polysaccharide sulfate mother liquor with distilled water to 5 concentrations: 0.2, 0.4, 0.6, 0.8, 1.0 mg / mL, 0.5 mL each.

[0087] 3. The test is divided into sample group, control group and blank group: take 3.5 ml of DPPH organic solution, add it to a 10 ml stoppered test tube, then ...

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Abstract

The invention belongs to the technical field of tobacco extracts, and particularly relates to a preparation method and application of tobacco polysaccharide sulfate. The preparation method mainly comprises the following steps: extracting tobacco polysaccharide and performing esterified modifying on the tobacco polysaccharide by sulfuric acid. The optimal sulfuric acid modification method is determined according to the monosaccharide constitution type of the tobacco polysaccharide, an optimal reaction condition and an optimal process for the sulfuric acid modification of the tobacco polysaccharide are determined by virtue of a series of experimental verification, the tobacco polysaccharide modified by the sulfuric acid is determined to be suitable for being used as a novel antioxidant by further determining the antioxidant activity of the tobacco polysaccharide modified by the sulfuric acid, so that a novel way is provided for the diversified utilization of tobacco.

Description

technical field [0001] The invention belongs to the technical field of tobacco extracts, and in particular relates to a preparation method and application of tobacco polysaccharide sulfate. Background technique [0002] Tobacco is an annual or perennial plant of the Solanaceae Nicotiana family, native to South America, with alternate single leaves and often sticky pilose. [0003] Tobacco is a controversial crop with huge economic benefits, and it is also the most widely planted non-food crop in the world. Tobacco cultivation and product processing play an important role in the development of the national economy. Tobacco was introduced very early in my country, and the output is quite large. So far, my country's tobacco planting area and output rank first in the world. At present, the research on tobacco leaves in our country is limited to the processing technology of cigarettes, and there are few research reports on other industries. However, because tobacco leaves cont...

Claims

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Application Information

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IPC IPC(8): C08B37/00C09K15/12
Inventor 许春平毛多斌马宇平苏东赢司俊玲荆晓燕杨琛琛孙斯文
Owner CHINA TOBACCO HENAN IND
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