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Pinellia ternata in vitro tuber planting method

A planting method and an in vitro technology, which are applied in the field of agricultural biology, can solve the problem of no in vitro tuber technology method of Pinellia ternata, and achieve the effects of large production and application value, energy saving, and shortening of the breeding cycle.

Inactive Publication Date: 2014-05-14
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no mature technical method for planting pinellia tubers in vitro

Method used

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  • Pinellia ternata in vitro tuber planting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Select isolated pinellia tubers with a diameter of 0.6-0.8cm from plant tissue culture, moderate length, high survival rate, easy operation, rinse with water for 3 times, sieve and wash with 8 meshes, and remove impurities on the surface of isolated pinellia tubers. Prevent bacterial infection, obtain fresh and directly plantable isolated pinellia tuber fresh products, and plant directly in the right season without storage; in the mixed solution of 25mg / L commercial rooting powder and 100mg / L agricultural streptomycin Soak in medium for 3 hours, control the soaking temperature at 24±3°C, and soak at the appropriate concentration and temperature of the mixed solution to avoid infection of Pinellia isolated tubers with various diseases and insect pests; choose river sand as the substrate in the germination and rooting medium, and the investment cost is low , easy to operate and easy to manage. Under the conditions of 121°C and 0.1Mpa, high temperature and high pressure dam...

Embodiment 2

[0030] The difference from Example 1 is that the concentration of commercial rooting powder is 100mg / L, and the concentration of agricultural streptomycin is 150mg / L. After 15 days of cultivation, the emergence rate is 99.98%.

Embodiment 3

[0032] The difference from Example 1 is that the culture conditions of the germination and rooting medium are: light intensity 2500 lux, light 18 h / d, temperature 25±1°C; after 15 days of culture, the emergence rate is 99.96%.

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Abstract

The invention discloses a pinellia ternata in vitro tuber planting method and belongs to the agricultural biotechnological field. The method comprises the steps of (1) acquisition of in vitro tubers, (2) cleaning and processing, (3) soaking, (4) selection and processing of a matrix in a germination accelerating and rooting culture medium, (5) planting, (6) germination accelerating and rooting culture, and (7) field transplanting. The method has the advantages that the breeding cycle is shortened, operability is high, energy is saved, pest and disease damage can be effectively prevented, investment cost is low, management is easy, season limitation is avoided, and cultivation of the in vitro tubers can be conducted at any seasons suitable for growth based on the fact that the pinellia ternata in vitro tubers can be grown all year around. Germination accelerating and seedling culture of the in vitro tubers are key techniques for connecting tissue culture with field planting, large-scale artificial cultivation of pinellia ternate is guaranteed in the true sense, and the production application value is high. With pinellia ternate serving as a model plant, the promotion of the technique will lay a foundation for genetic resource conservation and artificial cultivation of other precious plants.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a rapid germination planting method of isolated tubers obtained through tissue culture of the Chinese medicinal material pinellia. Background technique [0002] Pinellia belongs to the herbaceous plant of Araceae, and its tubers are used as medicine. It is one of the large traditional Chinese medicinal materials in my country and has a long history of medicinal use. According to reports, pinellia has various pharmacological effects such as reducing adverse reactions and eliminating dryness, relieving cough and reducing phlegm, anti-tumor, and anti-early pregnancy. In recent years, the market demand for pinellia has been increasing, and the predatory mining of wild resources has led to the depletion of pinellia resources, thus hindering the development of the entire Chinese medicine industry. In order to meet the needs of the Chinese herbal medicine market, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00
Inventor 陈集双张文涛许文飞金磊磊张本厚
Owner NANJING UNIV OF TECH
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