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Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method

A technology of insulin secretion and induction medium, applied in the field of biomedicine, can solve the problems of complicated induction process, long induction time, and small number of cells, and achieve the effect of broad clinical application prospects, no ethical problems, and high differentiation efficiency

Inactive Publication Date: 2014-04-30
青岛瑞思科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The induction and differentiation conditions of each laboratory are different, the induction and differentiation mechanism is not yet clear, the induction and differentiation efficiency is low, the islet secretion capacity is only about 1% of normal islets, and the induction process is complicated, the induction time is long, the number of cells obtained is small, and the function is low

Method used

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  • Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method
  • Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method
  • Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The inducer for inducing human adipose-derived mesenchymal stem cells into insulin-secreting cells in this example is composed of the following components in mass concentration ratio: Dazhu Rhodiola injection 20 g / L, nicotinamide 1.2g / L, taurine 0.50 g / L, GLP-1 30.5 mg / L.

[0029] The composition ratio of the induction medium containing the above-mentioned inducer is as follows: in each 1000ml of the induction medium, 20 g of Dazhu Rhodiola injection, 1.2 g of nicotinamide, 0.5 g of taurine, 30.5 mg of GLP-1, and the remaining The amount is human mesenchymal stem cell serum-free medium, and the above-mentioned components are mixed and then filtered and sterilized to prepare the inducer medium of the present invention.

[0030] The components of the inducer and induction medium of the present invention are all commercially available products: human mesenchymal stem cell serum-free medium, LONZA, 00190632; Dazhu Rhodiola injection, Tonghua Yusheng Pharmaceutica...

Embodiment 2

[0031] Example 2 This example is a method for inducing human adipose-derived mesenchymal stem cells into insulin-secreting cells, including the following steps:

[0032] 1. Preparation of adipose-derived mesenchymal stem cells:

[0033] 1. To receive adipose tissue, wipe the outer wall of the container containing adipose tissue with 75% alcohol.

[0034] 2. Dispense adipose tissue, each T175 culture bottle is divided into 50ml adipose tissue. With a 10ml pipette, remove the tip, first absorb the lower layer of red liquid in the fat collection bottle and discard it, and mix the remaining upper layer of fat before subpackaging.

[0035] 3. Wash fat tissue and remove blood cells. Add 100ml of sodium chloride injection to the T175 culture flask, tighten the cap, shake vigorously for 3 minutes to fully wash the adipose tissue, then stand still for 3-5 minutes to separate the different phases, and suck off the lower aqueous phase; repeat the above operation three times until The ...

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Abstract

The invention belongs to the field of biomedicine, and relates to an inducer for inducing human adipose derived stromal cells into insulin-secretion cells, an induction medium and a method. The inducer for inducing human adipose derived stromal cells into insulin-secretion cells consists of the following components: a rhodiola rosea injection, nicotinamide, taurine and GLP-1. Every 1,000ml of the induction medium containing the inducer comprises 20-40g of the rhodiola rosea injection, 0.97-1.47g of the nicotinamide, 0.25-0.50g of the taurine, 26.84-40.27mg of the GLP-1 and the balance being a human adipose derived stromal cell serum-free medium, which are mixed uniformly, filtered and degermed. According to the inducer for inducing human adipose derived stromal cells into insulin-secretion cells, the induction medium and the method, differentiation of the human adipose derived stromal cells into the insulin-secretion cells is induced by using the injection of a traditional Chinese medicine, namely, rhodiola rosea, and each component is safe and nontoxic. The method has few steps and is short in time and high in the inducing efficiency.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to an inducer, an induction medium and a method for inducing stem cells to differentiate into insulin-secreting cells. Background technique [0002] Diabetes mellitus (DM) is an endocrine and metabolic disease characterized by glucose and lipid metabolism disorders. According to the forecast released by the World Health Organization and the International Diabetes Federation, by 2030, there will be 370 million people with diabetes worldwide. At present, the number of DM patients in my country has exceeded 45 million, ranking second only to India. Although their etiologies are different, they all manifest as defects in the number and function of pancreatic islet cells, and ultimately require the use of insulin for treatment. Although due to the application of insulin, especially in recent years, some progress has been made in insulin dosage forms and administration routes, but practice has ...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/0775
Inventor 张炳强王秀芹孙全伟宋大鹏孙玉萍
Owner 青岛瑞思科生物科技有限公司
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