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Chinese sturgeon boule gene sequence and application thereof

A Chinese sturgeon, gene technology, applied in genetic engineering, plant genetic improvement, application and other directions

Inactive Publication Date: 2014-04-09
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Although studies have reported that the boule gene is expressed in the ovaries of nematodes, medaka and rainbow trout, it is mainly expressed in the testis

Method used

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  • Chinese sturgeon boule gene sequence and application thereof
  • Chinese sturgeon boule gene sequence and application thereof
  • Chinese sturgeon boule gene sequence and application thereof

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Embodiment Construction

[0018] Attached below Figures 1 to 4 The specific embodiments of the present invention are further described.

[0019] 1. Extraction of total RNA

[0020] The artificially cultured 4-year-old Chinese sturgeon was taken, anesthetized and exsanguinated, and the gonads were taken out, quick-frozen in liquid nitrogen, and then stored in a -80°C refrigerator. Take a spare sample and extract total RNA according to the instructions of the SV Total RNA Isolation System (Promega, USA) kit.

[0021] 2. Synthesis of SMART cDNA

[0022] Synthesize the first strand of Chinese sturgeon gonad SMART cDNA according to the SMARTer® RACE cDNA Amplification Kit instruction manual: (1) Take 2 μL of total RNA and mix with 1 μL of 3'-CDS Primer A / 5'-CDS Primer A, 72°C Incubate for 3 min at 42°C for 2 min. (2) Add 1 μL SMARTⅡA Oligo to the 5’RACE cDNA synthesis reaction tube, then mix and centrifuge. (3) Add 5×Buffer, DTT, RNase Inhibitor, dNTP, SMARTScribe™ Reverse Transcriptase to the reac...

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Abstract

The invention provides a Chinese sturgeon boule gene sequence and application thereof. A Chinese sturgeon boule gene has a cDNA (complementary Desoxvribose Nucleic Acid) sequence which is 2585bp in total length, has an open reading frame which is1065bp, codes 354 amino acids, and has a nucleotide sequence shown as SEQ ID No.1 and an amino acid sequence shown in SEQ ID No.2. The Chinese sturgeon boule gene sequence can be applied to identification, tracing and separation of germ cells of Chinese sturgeon, and lays a foundation for application and research on aspects of germ cellular transplantation, germplasm resource storage and the like of the Chinese sturgeon.

Description

technical field [0001] The invention relates to the field of biological engineering, in particular to a Chinese sturgeon boule gene sequence and use thereof. Background technique [0002] Chinese sturgeon ( Acipenser sinensis ) is an ancient large-scale anadromous migratory fish with the habit of breeding in fresh water and growing and developing in seawater. Due to the impact of human activities such as dam building, shipping, pollution and overfishing, the resources of Chinese sturgeon have declined sharply, and in 1988, it was listed as a national first-class protected animal. The Changjiang Institute has been engaged in artificial reproduction of Chinese sturgeon since the 1980s, and has realized large-scale artificial reproduction. However, this method requires long-term breeding of its parents, and requires a lot of human, material and financial resources, and there is also the death of broodstock. Therefore, it is necessary to seek other ways to protect this species...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N5/075C12N5/076C12Q1/68C07K14/46
Inventor 李创举叶欢危起伟岳华梅
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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