Preparation method of glucan embolism microspheres with CT (computed tomography) visualization function

Abstract

The invention relates to a preparation method of glucan embolism microspheres with a CT (computed tomography) visualization function. The preparation method is characterized by comprising the steps of dissolving polymethyl vinyl ether maleic anhydride grafted with beta-CD and modified glucan in water; uniformly stirring and then dropwise adding the solution into dimethyl silicone oil, wherein the volume ratio of a water phase to an oil phase is 1:5; under the condition of magnetic stirring, heating to 60-120 DEG C to react for 16-48 hours; after the reaction, standing and then removing supernatant fluid; cleaning microspheres deposited at the bottom of a container by using n-hexane; removing the remaining dimethyl silicone oil; and performing vacuum drying to remove the n-hexane on the surface to obtain gel microspheres with the particle size of 30+ / -5 microns and good dimensional homogeneity. The gel microspheres are added into a developer iodine solution or iodized oil, and the microspheres are discovered to have an obvious gray level through micro-CT observation.

Description

technical field [0001] The present invention relates to a method for preparing dextran embolization microspheres, in particular to a method for preparing dextran microspheres by reverse suspension polymerization. The obtained microspheres have the function of CT imaging and can be used for clinical arterial embolization treatment . Background technique [0002] Dextran is a natural non-animal oligosaccharide with glucose as the monosaccharide. It has a wide range of sources, is cheap, can be degraded by enzymes, can be used as a material for cell culture, and has good biocompatibility. β-cyclodextrin has a bowl-like structure, its large bowl is lipophilic, and the small bowl is hydrophilic, so it can be used as a carrier of iodine oil or iodine solution, and then realize CT imaging (Release Characteristics of Iodine Encapsulated in Cyclodextrins, Department of Biotechnology, Tottori University, 4-101 Koyamacho-minami, 680-8552, Tottori, Japan). Polymethyl vinyl ether malei...

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Problems solved by technology

[0003] There are currently four types of embolization consumables for transcatheter vascular embolization on the market: 1. Sponge embolization consumables, such as: gelatin sponge and polyvinyl alcohol. Among them, gelatin sponge belongs to short-term embolization consumables and is easily absorbed by the body; Long-term embolization consumables mainly rely on imports and are expensive; 2. Liquid embolization consumables, such as: absolute ethanol, sodium morrhuate, super-liquefied lipiodol, and Pingyangmycin lipiodol emulsion. After the human body, it reacts with tissues and causes serious damage to human tissues; cod liver oil sodium is a long-term embolization consumable, but it can only be applied to thicker blood vessels; super liquefied lipiodol is a short-term embolization consumable, which is easy to be absorbed by the body; Pingyangmycin lipiodol Emulsions are medium-term embolization consumables, containing radioactive substances, have the effect of chemotherapy, are harmful to human body, and are expensive; Imported, expensive; 4. Large-scale embolization consumables, the main products are: steel rings, which are long-term embolization consumables, but only suitable for thicker blood vessels

[0004] However, the preparation process of microsphere embolic agents on the market is relatively complicated, and it is difficult to mass-produce them. They are also mixed with toxic chemical substances, which may cause antigenicity, local tissue and systemic toxic reactions, and will be destroyed if they do not exist long enough in the body. degradation

Some microspheres have poor mechanical properties and cannot withstand the impact of blood flow, so it is difficult to achieve the effect of blocking blood vessels. In addition, the particle size cannot meet the diameter of the artery.

Clinically, lipiodol must be added for imaging, which is cumbersome and inconvenient

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