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Process method for extracting general flavone of golden camellia leaves by CO2 supercritical method

The technology of a golden flower tea and a process method is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, food science, etc., to achieve the effects of good safety, improved economic and social benefits, and high yields

Inactive Publication Date: 2014-04-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far no CO has been found in the literature search 2 A report on the process of extracting total flavonoids from Jinhua tea leaves by supercritical method

Method used

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  • Process method for extracting general flavone of golden camellia leaves by CO2 supercritical method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take fresh Jinhua tea leaves, dry them in the shade, crush them, and sieve them. Take 150 g of the powder and add it to the extraction tank of a supercritical extractor with 75% ethanol as the carrier agent, the extraction temperature is 45 °C, the pressure of the extraction tank is 35 MPa, CO 2 The flow rate is 15 L / h; the temperature of the primary separation tank is 25 ℃ and the pressure is 8 MPa; the temperature of the secondary separation tank is 25 ℃ and the pressure is 6 MPa; the extraction time is 3 h, and the dark brown total flavonoids of golden camellia tea are obtained ethanol solution. Ethanol was recovered by distillation under reduced pressure and freeze-dried to obtain a dark brown total flavonoid extract of Camellia japonica. The yield of flavonoids was 3.81%.

[0020] The analysis and detection method of total flavonoids in Camellia japonica is as follows: Accurately weigh 25.4 mg of rutin reference substance, put it in a 100 mL measuring bottle, add...

Embodiment 2

[0024] Take fresh Jinhua tea leaves, dry them in the shade, crush them, and sieve them. Take 150 g of the powder and put it into the extraction tank of a supercritical extractor, using 65% ethanol as the carrier agent, the extraction temperature is 35 °C, the pressure of the extraction tank is 25 MPa, CO 2 The flow rate is 15 L / h; the temperature of the primary separation tank is 25 ℃ and the pressure is 8 MPa; the temperature of the secondary separation tank is 25 ℃ and the pressure is 6 MPa; the extraction time is 2 h, and the total flavonoids of dark brown golden camellia tea are obtained ethanol solution. Ethanol was recovered by distillation under reduced pressure and freeze-dried to obtain a dark brown extract of total flavonoids from Camellia japonica. The yield of total flavonoids was 0.781%.

Embodiment 3

[0026] Take fresh Jinhua tea leaves, dry them in the shade, crush them, and sieve them. Take 150 g of the powder and add it to the extraction tank of a supercritical extractor, use 65% ethanol as the carrier agent, the extraction temperature is 45 °C, the pressure of the extraction tank is 35 MPa, CO 2 The flow rate is 15 L / h; the temperature of the primary separation tank is 25 ℃ and the pressure is 8 MPa; the temperature of the secondary separation tank is 25 ℃ and the pressure is 6 MPa; the extraction time is 3 h, and the total flavonoids of dark brown golden camellia tea are obtained ethanol solution. Ethanol was recovered by distillation under reduced pressure and freeze-dried to obtain a dark brown total flavonoid extract from Camellia japonica. The yield of total flavonoids was 3.694% after detection.

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Abstract

The invention discloses a process method for extracting general flavone of golden camellia leaves by a CO2 supercritical method. The process method comprises the following steps: 1) crushing golden camellia powder to 20-100 meshes and placing the powder in an extraction tank, wherein 55-85% ethanol is taken as a carrying agent and supercritical CO2 is taken as a solvent, thereby obtaining ethanol liquor of general flavone of golden camellia leaves under a condition that the temperature of the extraction tank is 35-55 DEG C, the pressure is 25-35MPa, the CO2 flow is 10-20L / h, the temperature of a first level separation tank is 25 DEG G, the pressure is 6-8MPa, the temperature of a second level separation tank is 25 DEG G, the pressure is 6MPa, and the extracting time is 2-4 hours; and 4) decompressing and distilling to recover ethanol, and cooling and drying to obtain a dark brown extract of general flavone of golden camellia leaves. The general flavone extracted by the method is free from residual organic solvent, and the method is simple, convenient and feasible and high in yield, and the yield can reach 3.81%. The method can be widely applied to the fields such as foods and medicines, and the economical and social benefits of golden camellia can be remarkably improved.

Description

technical field [0001] The invention relates to a method for extracting biologically active substances of Chinese herbal medicines, in particular to CO 2 The invention discloses a process method for extracting total flavonoids of golden camellia tea leaves by a supercritical method, belonging to the technical field of traditional Chinese medicines. Background technique [0002] Camellia [ Camellia chrysantha (Hu) Tuyama] is a plant belonging to the family Theaceae, Camellia, and Camellia. It was first discovered in Guangxi in 1960. Because of its golden waxy flowers, golden petals and jade stamens, and fresh and pretty, Dr. Hu Xiansu, a famous Chinese botanist, named it Golden Camellia in 1965. As a rare plant resource in the world (distributed only in the border area between Guangxi and Vietnam), golden camellia has been used as a traditional folk Chinese herbal medicine in the local area for the treatment of pharyngitis, dysentery, high blood pressure, prevention of tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A23L1/30A23L33/105
Inventor 周远明
Owner QINGDAO AGRI UNIV
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