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Preparation method and application method of gl and tcm199 as bovine semen diluent and freezing liquid

A technology of TCM199 and preparation method, which is applied in the field of preparation of GL and TCM199 as bovine semen diluent and freezing liquid, can solve the problems of high viscosity, complex operation technology, large particles, etc., achieve simple method, improve conception rate, reduce particle effect

Inactive Publication Date: 2017-01-18
GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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Problems solved by technology

[0007] The purpose of the embodiments of the present invention is to provide a method for preparing and using GL and TCM199 as bovine semen diluent and freezing liquid, aiming at solving the problems of the existing bovine semen diluent with large particles, high viscosity and complex operation techniques

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  • Preparation method and application method of gl and tcm199 as bovine semen diluent and freezing liquid
  • Preparation method and application method of gl and tcm199 as bovine semen diluent and freezing liquid
  • Preparation method and application method of gl and tcm199 as bovine semen diluent and freezing liquid

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0029] Such as figure 1 Shown, the preparation method of the bovine semen dilution liquid of the embodiment of the present invention comprises the following steps:

[0030] S101: Accurately weigh 2 grams of TCM199, 40 grams of Tris, 20 grams of citric acid, 0.1 grams of sodium pyruvate, 5 grams of fructose, and 1 gram of 800,000 units of penicillin sodium with an electronic balance;

[0031] S102: Put the above components into a 1000 ml volumetric flask, add 50...

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Abstract

The invention discloses a preparation method and a using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid. The preparation method comprises preparation of the bovine semen diluent and preparation of the bovine semen refrigerating fluid. The using method comprises the steps of preparing the bovine semen diluent and preparing the bovine semen refrigerating fluid; collecting semen; performing preliminary dilution; performing secondary dilution; and performing refrigeration storage. According to the preparation method and the using method, the defreezing activity of frozen bovine semen is improved, the bovine artificial insemination conception rate is increased, the viscosity of the bovine semen diluent is reduced, and the granular size of the bovine semen diluent is lowered; the operation is simplified; the preparation method and the using method can be used under various large-scale production conditions; the standardization and practicability level of refrigeration of the bovine semen in various scales and the defreezing activity of bovine capillary refrigerated semen are improved; the aberration rate of the defrozen semen is lowered, and the bovine artificial insemination conception rate is increased.

Description

technical field [0001] The invention belongs to the technical field of bovine artificial insemination, and in particular relates to a method for preparing and using GL and TCM199 as bovine semen diluent and freezing liquid. Background technique [0002] Cattle breeding methods include self-crossing and artificial insemination. Artificial insemination is divided into fresh mating and frozen mating. Remote and centralized artificial insemination using thin tube frozen semen is the most important method of cattle breeding. Therefore, the quality of the frozen sperm is the key link to ensure the conception rate of artificial insemination. The quality of straw frozen semen is affected by the health status of the donor bull, feeding level, semen quality, production process of straw frozen semen and storage conditions. In these links, except for the production of straw frozen semen, manual control can be carried out, while the straw The production process of frozen essence is grea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 桑国俊邱忠玉郭海龙王珂容维中保国俊
Owner GANSU ANIMAL HUSBANDRY & VETERINARY MEDICINE INST
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