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Method for improving stability of carbon dioxide detection reagent

A technology for detecting reagents and carbon dioxide, applied in the field of carbon dioxide detection reagent protective film composition, to achieve the effect of improving stability and excellent stability

Active Publication Date: 2014-04-02
上海执诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent does not address the use of CO 2 Study on Stability of Detection Reagents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Accurately weigh 5g of glycerin and 0.8g of ethylene glycol, and then dissolve them in 100ML of carbon dioxide detection reagent according to the above weighing sequence, then use 1mol / l hydrochloric acid or sodium hydroxide to adjust the pH to 8.0, and then add 10g of simethicone, A film composition composed of 9 g of light paraffin oil and Tween-205 g is added to the surface of the liquid, so that it spreads on the surface of the liquid to form a connected protective film. For the carbon dioxide detection kit used in the carbon dioxide protective film composition prepared above, the performance of the reagent is still good after the reagent is opened and placed in the reagent compartment for more than 30 days.

Embodiment 2

[0018] Accurately weigh 0.5g of polyoxyethylene lauryl ether and 3.5g of glycerin, and then dissolve it in 100ML of carbon dioxide detection reagent according to the above weighing sequence, then use 1mol / l hydrochloric acid or sodium hydroxide to adjust its pH to 8.0, and then add dimethyl A film composition composed of 10g of silicone oil, 5g of mineral oil and 205g of Tween is added to the surface of the liquid so that it spreads on the surface of the liquid to form a connected protective film. For the carbon dioxide detection kit used in the carbon dioxide protective film composition prepared above, the performance of the reagent is still good after the reagent is opened and placed in the reagent compartment for more than 30 days.

Embodiment 3

[0020] Accurately weigh 0.1g of glycerin and 20g of ethylene glycol, and then dissolve them in 100ML of carbon dioxide detection reagent according to the above weighing sequence, then use 1mol / l hydrochloric acid or sodium hydroxide to adjust the pH to 6.5, and then add 90g of simethicone, A film composition consisting of 0.1 g of light paraffin oil and 200.1 g of Tween is added to the surface of the liquid so that it spreads on the surface of the liquid to form a connected protective film. Place the treated carbon dioxide detection reagent in the reagent compartment, and the performance is still good after more than 30 days.

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PUM

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Abstract

The invention provides a method for improving the stability of a carbon dioxide detection reagent; after the method is adopted, the stability and the biological activity of the carbon dioxide detection reagent can be maintained for a long time and the biological activity of the carbon dioxide detection reagent can be maintained after the carbon dioxide detection reagent is decapped. According to the method, an organosilicon polymer, mineral oil or light paraffin oil, a surface active agent and a pH value regulator are used for reducing the contact between carbon dioxide in outside air and the reagent, so that the stability of the carbon dioxide biochemical reagent can be remarkably improved. After the method for improving the stability of the carbon dioxide detection reagent is adopted, a carbon dioxide detection kit has the relatively excellent stability, so that the demands of scientific research and medical examination can be relatively well met.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for enhancing the stability of a carbon dioxide detection reagent and a protective film composition for the carbon dioxide detection reagent. Background technique [0002] Carbon dioxide in the blood (CO 2 ) mainly in the form of bicarbonate (HCO 3 - ) form, accounting for about 95%. CO in the blood 2 The content plays an important role in maintaining the acid-base balance of the body, and provides an important basis for the diagnosis and treatment of acid-base balance disorders. [0003] blood CO 2 It is a routine item of clinical biochemical testing, which can better understand the acid-base balance in the body. When metabolic alkalosis such as pyloric obstruction, adrenal hyperfunction, taking too much alkaline drugs, etc.; respiratory acidosis such as emphysema, pulmonary fibrosis, airway obstruction, etc. will lead to CO in the blood 2 content increase. Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 王辉王会王军峰
Owner 上海执诚生物科技有限公司
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