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A kind of Ganoderma ginkgo fruit wine and its preparation method

A technology of Ganoderma lucidum and ginkgo, applied in the field of bioengineering, can solve the problems of easy pollution, low conversion of ginkgo, high fermentation cost, etc.

Inactive Publication Date: 2015-12-09
JIANGSU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wine prepared by these two methods, because the liquid fermentation is used in the biotransformation stage, the fermentation cost is high, easy to pollute, and the amount of transformed ginkgo is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of strains in liquid shake flasks: 20 grams of sucrose, 50 grams of soybean cake powder, 50 grams of corn steep liquor, 60 grams of bran, 0.6 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 40mL bottle, sterilized at 100°C for 60 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultured at 22°C for 10 days at a rotating speed of 60 rpm, to obtain strains of Ganoderma lucidum liquid shake flasks;

[0018] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.6 grams of potassium dihydrogen phosphate, and 0.5 grams of magnesium sulfate according to the volume of the seed tank , 7 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferred to the see...

Embodiment 2

[0022] Preparation of strains in liquid shake flasks: 60 grams of sucrose, 30 grams of soybean cake powder, 30 grams of corn steep liquor, 30 grams of bran, 0.3 grams of potassium dihydrogen phosphate, 0.3 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 80mL bottle, sterilized at 115°C for 40 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultured at 120 rpm at a temperature of 28°C for 7 days to obtain a strain of Ganoderma lucidum liquid shake flask;

[0023] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 60 grams of sucrose, 25 grams of soybean cake powder, 31 grams of corn steep liquor, 30 grams of bran, 0.25 grams of potassium dihydrogen phosphate, and 0.25 grams of magnesium sulfate according to the volume of the seed tank , 4.1 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferred to the se...

Embodiment 3

[0027] Preparation of strains in liquid shake flasks: 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.06 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 140mL bottle, sterilized at 128°C for 55 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultivated at 180 rpm at a temperature of 33°C for 5 days to obtain the ganoderma lucidum liquid shake flask strain;

[0028] Preparation of liquid expanded culture strain: according to the volume of the seed tank, take by weighing 20 grams of sucrose, 45 grams of soybean cake powder, 45 grams of corn steep liquor, 60 grams of bran, 0.06 grams of potassium dihydrogen phosphate, and 0.05 grams of magnesium sulfate according to the volume of the seed tank , 2 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transfer...

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PUM

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Abstract

The invention discloses a ganoderma lucidum-ginkgo fruit wine and a preparation method thereof, and the process relates to the field of biological engineering technology. The ganoderma lucidum-ginkgo fruit wine is prepared by taking ginkgo as main raw material and by adopting a solid fermentation technology and a wine-brewing process. The preparation method comprises the steps: cutting ginkgo into small lumps with the size of 3-5 mm, introducing a 5-30% ganoderma lucidum seed liquid after sterilization, carrying out sealed fermentation with a preservative film, adding cooked rice cooked from rice having the weight of 30-70% of the ganoderma lucidum fermentation product weight, distiller yeasts with the weight of 1-5% of the rice weight and water with the amount being 2-5 times the rice weight, and after fermenting at the temperature of 10-30 DEG C for 10-50 days, filtering and blending the fermentation liquid to obtain the ganoderma lucidum-ginkgo fruit wine. The alcoholic strength of the wine is 8-30%, the total flavone content is 1-15 mg / L, the ginkgolides content is 10-150 mg / L, the ganoderma triterpenes content is 50-500 mg / L, the protein content is 60-500 mg / L, the soluble sugar content is less than 2.0%, the polysaccharide content is 100-800 mg / L, and the saponin content is 20-500 mg / L. The wine has anti-oxidation and liver-protecting efficacies.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method and application for producing ginkgo ginkgo fruit wine by using ganoderma lucidum to transform ginkgo ginkgo fruit by solid fermentation technology, and undergoing two fermentations, filtering and blending. Background technique [0002] The main components of ginkgo biloba are protein, starch and fat, and it also contains flavonoids, terpenoids, ginkgolic acid, hydrogenated ginkgo acid, hydrogenated ginkgo acid, ginkgo alcohol, ginkgool and polysaccharides. Ancient books of traditional Chinese medicine in my country have always listed ginkgo as an important medicinal material. Ginkgo acid inhibits a variety of bacilli and skin fungi, and has different degrees of resistance to staphylococcus, streptococcus, diphtheria, anthrax bacillus, Bacillus subtilis, Escherichia coli, typhoid bacteria, etc. inhibitory effect. Ginkgo phenolic acid extracted from fresh ginkgo fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12G3/02
Inventor 张志才郑惠华任晓峰张才九陈惠谢德中
Owner JIANGSU UNIV
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