A kind of ganoderma fruit wine and preparation method thereof
A technology of Ganoderma fruit wine and Ganoderma lucidum, applied in the field of bioengineering, can solve problems such as pollution, high cost of Ganoderma lucidum wine, and long production cycle
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Embodiment 1
[0020] Preparation of strains in liquid shake flasks: 20 grams of sucrose, 50 grams of soybean cake powder, 50 grams of corn steep liquor, 60 grams of bran, 0.6 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 40mL bottle, sterilized at 100°C for 60 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultured at 22°C for 10 days at a rotating speed of 60 rpm, to obtain strains of Ganoderma lucidum liquid shake flasks;
[0021] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.6 grams of potassium dihydrogen phosphate, and 0.5 grams of magnesium sulfate according to the volume of the seed tank , 7 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferred to the se...
Embodiment 2
[0025] Preparation of strains in liquid shake flasks: 60 grams of sucrose, 30 grams of soybean cake powder, 30 grams of corn steep liquor, 30 grams of bran, 0.3 grams of potassium dihydrogen phosphate, 0.3 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 80mL bottle, sterilized at 115°C for 40 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultured at 120 rpm at a temperature of 28°C for 7 days to obtain a strain of Ganoderma lucidum liquid shake flask;
[0026] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 60 grams of sucrose, 25 grams of soybean cake powder, 31 grams of corn steep liquor, 30 grams of bran, 0.25 grams of potassium dihydrogen phosphate, and 0.25 grams of magnesium sulfate according to the volume of the seed tank , 4.1 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferred to the see...
Embodiment 3
[0031] Preparation of strains in liquid shake flasks: 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.06 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 140mL bottle, sterilized at 128°C for 55 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganodermal lucidum ), cultivated at 180 rpm at a temperature of 33°C for 5 days to obtain the ganoderma lucidum liquid shake flask strain;
[0032] Preparation of liquid expanded culture strain: according to the volume of the seed tank, take by weighing 20 grams of sucrose, 45 grams of soybean cake powder, 45 grams of corn steep liquor, 60 grams of bran, 0.06 grams of potassium dihydrogen phosphate, and 0.05 grams of magnesium sulfate according to the volume of the seed tank , 2 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferr...
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