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Anatase type TiO2 nano particle based pH sensor and pH value detection method

A nanoparticle and anatase-type technology, which is applied in the field of pH sensors and pH value detection based on anatase-type TiO2 nanoparticles, can solve the problems of complex detection operations and achieve high detection accuracy, good reproducibility, The effect of signal stabilization

Active Publication Date: 2014-03-26
THE SECOND HOSPITAL OF NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some deficiencies in the glass electrode method. For example, the temperature and two standard pH buffer solutions need to be calibrated first during the measurement process, and then the pH value of the liquid to be tested is measured. The detection operation for a small amount of samples is more complicated.

Method used

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  • Anatase type TiO2 nano particle based pH sensor and pH value detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The present invention only uses ITO conductive glass as the embodiment of the conductive carrier, and uses the urease catalyzed reaction system as the embodiment of biosensing, but is not limited to the specific content of the embodiment.

[0042] The first step: commercial anatase TiO 2 The nanoparticles were uniformly dispersed in the water phase by ultrasonication for 30 minutes.

[0043] Step 2: Cut the commercialized ITO conductive glass into appropriate sizes, soak in acetone and ethanol for 2 hours each, rinse thoroughly with double-distilled water, and soak in double-distilled water for later use.

[0044] The third step: ultrasonically dispersed anatase TiO 2 The nanoparticles are drip-coated on the bottom of the ITO glass to form a 0.5cm×1cm film, which is dried at room temperature to form a flat and uniform film, which is constructed as a sensitive element of the pH sensor and stored at room temperature in the dark.

[0045] The fourth step: the constructed...

Embodiment 2

[0048] Following the experimental steps of Example 1, continue to build a biosensor capable of measuring both the pH value and the concentration of the active substance urea in the biological system.

[0049] Step 6: Modify the 0.1%wt chitosan aqueous solution on the pH sensor TiO 2 Above the nanoparticle modification area, form a 0.5cm×1cm film, dry it in a refrigerator at 4°C to form a uniform film; spread 0.1M pH7.4 phosphate buffer solution with urease and 2%wt glutaraldehyde on On the chitosan film, urease was further modified on the ITO conductive glass by amino-amino cross-linking reaction. Apply the three-electrode system mentioned in the third step to obtain a pH biosensor ( figure 2 ).

[0050] Step 7: Select a suitable supporting electrolyte. When different concentrations of substrate urea are added to the solution, the enzyme-catalyzed reaction can cause changes in the pH value of the detection environment, and the electrochemiluminescence peak potential of the ...

Embodiment 3

[0052] The present invention only uses ITO conductive glass as the embodiment of the conductive carrier, and uses the acetylcholinesterase catalytic reaction system as the embodiment of biosensing, but is not limited to the specific content of the embodiment. Following the experimental steps of Example 1, continue to construct a biosensor capable of measuring both the pH value and the concentration of the active substance acetylcholine in the biological system.

[0053] Step 6: Modify the 0.1%wt chitosan aqueous solution on the pH sensor TiO 2 Form a 0.5cm×1cm film on the surface of the nanoparticle modification area, and dry it in a refrigerator at 4°C to form a uniform film; put 0.1M pH7.4 phosphate buffer dissolved in acetylcholinesterase and 2%wt glutaraldehyde Spread on the chitosan film, and further modify the urease on the ITO conductive glass through amino-amino cross-linking reaction. Apply the three-electrode system mentioned in the third step to obtain a pH biosens...

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Abstract

The invention discloses an anatase type TiO2 nano particle based pH sensor and a pH value detection method. TiO2 nano particles are ultrasonically dispersed in a water phase, dispensed on the surface of ITO (indium tin oxide) conductive glass and dried at the room temperature, and a formed uniform film forms a sensitive element of the pH sensor. On the basis of an electrochemiluminescence technology, the ITO conductive glass modified by the TiO2 nano particles is taken as a working electrode, a three-electrode system is adopted, and an integral pH sensor is obtained. An electrochemiluminescence peak potential of the working electrode shifts negatively with the rising of the pH value, so that the pH value of a solution is detected according to the principle. The pH sensor displays stable and repeatable detection signals, and the application range can cover eight pH units. Further, urease is modified above the TiO2 modification area on the ITO glass, a biological sensing application model of the pH sensor is obtained, and the pH sensor can be applied to concentration monitoring of active substances in a biology system.

Description

technical field [0001] The invention relates to an anatase-based TiO 2 Nanoparticle pH sensor and pH detection method. Using TiO 2 The characteristics of the nanoparticles, after a simple drop-coating and drying operation, a pH sensor was obtained. Electrochemiluminescence technology was used to record the behavior of its luminescence potential changing with pH, ​​and a pH value detection method was obtained. The principle of pH value detection can further construct a pH biosensor to detect the concentration of the enzyme substrate. Background technique [0002] TiO 2 Nanomaterials have the advantages of large specific surface area, active surface adsorption characteristics, and high photocatalytic efficiency. They have been widely used in the production of fuel cells and sewage treatment. Its surface properties are active and easy to undergo secondary modification by adsorption deposition for higher photocharge harvesting efficiency. In addition, TiO 2 The optoelectr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 刘璇姜晖赵伟王念跃
Owner THE SECOND HOSPITAL OF NANJING
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