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A preparation method of ultra-high deacetylation degree chitosan using Antarctic krill as raw material

An Antarctic krill and deacetylation technology, which is applied in the field of polymer polysaccharide preparation, can solve the problems of difficulty in meeting the demand for high-quality chitosan, industrial application and human health impact, and poor repeatability of chitosan products. The effect of low cost, less impurities and simple preparation method

Active Publication Date: 2016-01-20
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) The waters where offshore crustaceans live are polluted by chemical plants, pesticides and domestic sewage. Shrimp and crab shells contain a lot of harmful elements, and the prepared chitosan contains more impurities, which will cause serious harm to industrial applications and human health. (2) There are many kinds of shrimps and crabs in the sea, and the chitin content in the shells of shrimps and crabs varies greatly. The reproducibility of the chitosan products is poor, the quality varies greatly, it is difficult to meet the demand for high-quality chitosan; (3) the degree of deacetylation of the prepared chitosan is 55% to 85%, and the degree of deacetylation is not high. It greatly limits the application of chitosan in the fields of medicine and biology; (4) Chitin needs to be decolorized during the preparation process, which is cumbersome

Method used

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  • A preparation method of ultra-high deacetylation degree chitosan using Antarctic krill as raw material
  • A preparation method of ultra-high deacetylation degree chitosan using Antarctic krill as raw material
  • A preparation method of ultra-high deacetylation degree chitosan using Antarctic krill as raw material

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preparation example Construction

[0034] The invention provides a preparation method of chitosan with ultra-high degree of deacetylation using Antarctic krill as raw material, comprising the following steps:

[0035] (1) Demineralization: take dry Antarctic krill shells, soak in acid solution, filter, collect shrimp shells, wash with water until neutral, and obtain demineralized shrimp shells;

[0036] (2) Removal of protein and lipids: The demineralized shrimp shells obtained in step (1) are placed in lye and heated, and then filtered to collect the shrimp shells, washed with water until neutral, to obtain the demineralized shrimp shells obtained in step (1). shrimp shell;

[0037] (3) Demineralization: soaking the shrimp shells obtained in step (2) after removing proteins and lipids in an acid solution, filtering, collecting the shrimp shells, washing with water until neutral, to obtain demineralized shrimp shells;

[0038] (4) Removal of protein and lipids: the demineralized shrimp shells obtained in step ...

Embodiment 1

[0054] A preparation method of ultra-high degree of deacetylation chitosan with Antarctic krill as raw material, comprising the following steps:

[0055] (1) Demineralization: Take 10 g of dry Antarctic krill shells, soak them in 100 mL of 2mol / L dilute hydrochloric acid for 1 hour at room temperature, filter, collect shrimp shells, and wash with water until neutral to obtain demineralized shrimp shells;

[0056] (2) Removal of protein and lipids: Take the demineralized shrimp shells obtained in step (1), place them in 150 mL of 1 mol / L sodium hydroxide solution, heat and boil for 1 hour, then filter, collect the shrimp shells, wash with water until neutral, and obtain Shrimp shell after protein and lipid removal;

[0057] (3) Demineralization: soak the shrimp shells obtained in step (2) after removing proteins and lipids in 100 mL of 2 mol / L dilute hydrochloric acid for 1 hour at room temperature, then filter, collect the shrimp shells, and wash with water until neutral to obta...

Embodiment 1 comparative example 1 comparative example 2

[0065] Viscosity / cps505±8.66325±7.2254±0.96

[0066] The viscosity of the chitosan prepared in this example is about 505cps, and the viscosity of Comparative Example 1 and Comparative Example 2 are about 325cps and 54cps respectively, indicating that the chitosan prepared by the present invention has a high relative molecular mass.

[0067] The chitosan prepared in this example and the chitosan of Comparative Example 1 were subjected to Fourier transform infrared test, and the test results refer to figure 1 ,from figure 1 It can be seen that Example 1 is at 3400cm -1 The stretching vibration peak of H-O appears at 2960cm -1 ~2840cm -1 C-H stretching vibration peaks of methyl and methine appear at 1655cm -1 C=O stretching vibration peak appears at 1604cm -1 N-H bending vibration peak appears at 1379cm -1 C-H bends and CH 3 Symmetrical deformation vibration peak at 1323cm -1 C-N stretching vibration peak appears at 1260cm -1 ~1000cm -1 The C-O stretching vibration peak...

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Abstract

The invention provides a method for preparing chitosan with ultrahigh deacetylation degree from euphausia superba as a raw material. The method sequentially comprises the following steps: removing mineral substances, removing proteins and lipids, removing mineral substances, removing proteins and lipids, deacetylating and purifying. According to the method, the euphausia superba is taken as the raw material, and the prepared chitosan has few impurities and a high deacetylation degree, and can be applied to the fields of medicines and biology.

Description

technical field [0001] The invention relates to a preparation method of high molecular polysaccharide, in particular to a preparation method of chitosan. Background technique [0002] Chitosan is obtained by deacetylation of chitin. It is the only alkaline polysaccharide among natural polysaccharides. This polymer material has the advantages of good biocompatibility and microbial degradability. Widely concerned by all walks of life. [0003] At present, the production of chitosan mainly uses offshore shrimp and crab shells as raw materials, and uses dilute acid and dilute alkali treatment to extract chitosan, but this method has the following problems: [0004] (1) The waters where the offshore crustaceans live are polluted by chemical plants, pesticides and domestic sewage. Sea shrimp and crab shells contain a lot of harmful elements, and the prepared chitosan contains many impurities, which are harmful to industrial applications and human health. (2) There are many kinds...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 赵晓丽黄卓瑶吴帅潘浩波
Owner SHENZHEN INST OF ADVANCED TECH
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